|
Status |
Public on Nov 29, 2008 |
Title |
Siberian hamster hypothalamic 4K array |
Technology type |
spotted DNA/cDNA |
Distribution |
non-commercial |
Organism |
Phodopus sungorus |
Manufacturer |
Rowett Research Institute |
Manufacture protocol |
This array has been made from a subtracted cDNA library of Siberian hamster hypothalamic tissue. Transcripts expressed in liver and kidney were subtracted from hypothalamic mRNA. A total of 5000 individual colonies were picked from the library using a robotic colony picker and these were amplified by PCR using Rsa1 and Rsa2 primers. Approximately 4000 clones containing single inserts of 250 to 1000bp sizes were identified and used for the array. Clones have been saved as glycerol stocks.
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Support |
glass |
Coating |
aminosilane |
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Description |
The array has been printed in 48 blocks (4 columns and 12 rows), each block contains 255 features; 17 columns with 5 rows in triplicate at 0.25mm spacing. Mixed Arabidopsis thaliana cDNAs have been printed as landmarks in the outer corners of the 4 corner blocks. Individual and mixed A. thaliana cDNAs have also been printed within the array.
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|
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Submission date |
Nov 18, 2007 |
Last update date |
Nov 29, 2008 |
Contact name |
Gill Campbell |
E-mail(s) |
G.Campbell@abdn.ac.uk
|
Phone |
+44 (0) 1224 716627
|
Fax |
+44 (0) 1224 716647
|
URL |
http://www.rowett.ac.uk
|
Organization name |
University of Aberdeen
|
Department |
Rowett Institute of Nutrition and Health
|
Street address |
Greenburn Road
|
City |
Aberdeen |
ZIP/Postal code |
AB21 9SB |
Country |
United Kingdom |
|
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Samples (5) |
GSM259449, GSM259451, GSM259453, GSM259454, GSM259455 |
Series (1) |
GSE10278 |
Photoperiod regulates genes encoding melanocortin 3 and serotonin receptors, and secretogranins expressed in the dmpARC of the Siberian hamster |
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Data table header descriptions |
ID |
|
MetaColumn |
Column number of the block within the array |
MetaRow |
Row number of the block within the array |
Column |
Spot column within the block |
Row1 |
Spot row within the block for first replicate |
Row2 |
Spot row within the block for second replicate |
Row3 |
Spot row within the block for third replicate |
BioSequence type |
Type of DNA sequence |
Reporter Identifier |
Clone ID (spot plate and well position) |
Reporter usage |
Type of reporter |
Gene ID |
Clone identified as part of this gene |
Accession number |
Gene database number |