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Status
Public on Jul 03, 2006
Title
White tiling and microRNA array
Technology type
in situ oligonucleotide
Distribution
custom-commercial
Organism
Drosophila melanogaster Manufacturer
LC Sciences (Houston, TX, USA)
Manufacture protocol
MicroRNA probes were designed by LC Sciences (based on Sanger Institute miRBase repository sequences on November 17, 2005). LNA-modified RNA probes were synthesized in situ using photogenerated acid coupled with conventional DMT chemistry (LNA-modification ensures Tm-normalization). Synthesis was performed on a µParaflo microfluidic chip.http://www.lcsciences.com for additionnal details.
Catalog number
UM_050548
Contributor(s)
Vagin VV , Sigova A , Li C , Seitz H , Gvozdev V , Zamore PD Submission date
May 25, 2006
Last update date
Jul 03, 2006
Contact name
Hervé Seitz
Phone
(508)856 1220
Fax
(508)856 2003
URL
http://www.umassmed.edu/bmp/faculty/zamore.cfm?start=0& Organization name
University of Massachusetts Medical School
Department
Biochemistry and Molecular Pharmacology
Lab
870 P, Lazare Research Building
Street address
364 Plantation Street
City
Worcester
State/province
MA
ZIP/Postal code
01605-2324
Country
USA
Samples (1)
GSM111153 Series (1)
GSE4932 A small RNA mechanism distinct from the RNAi and microRNA pathways silences selfish genetic elements in Drosophila.
Data table header descriptions ID Probe_ID Name of the detected RNA
SEQUENCE Sequence of the detected RNA
Row Position on array (row)
Column Position on array (column)
Position Position on array (single-cell format)
SPOT_ID
Data table ID Probe_ID SEQUENCE Row Column Position SPOT_ID 1
1
1
1-1:1
-- empty
2
dme-miR-1
UGGAAUGUAAAGAAGUAUGGAG
1
2
1-1:2
3
dme-miR-276b
UAGGAACUUAAUACCGUGCUCU
1
3
1-1:3
4
s-dme-bantam
CCAAAUCGAAAACCGGUUUGACU
1
4
1-1:4
5
s-dme-mir-263b
GGUUCUGCGGGUGCCAAAAC
1
5
1-1:5
6
snoRNA:U21
AUUGUGAUUAUUCAAGUUUAUUU
1
6
1-1:6
7
ANTIwhiteExon3.fa 1
AAGCCGUCGACUUCUUUUCCUA
1
7
1-1:7
8
ANTIwhiteExon3.fa 129
GAAGCUGUCGCAGAAGGGCAAG
1
8
1-1:8
9
ANTIwhiteExon3.fa 257
UCCGGCGGAGAAAGGAAGCGUC
1
9
1-1:9
10
1
10
1-1:10
-- empty
11
ANTIwhiteExon3.fa 385
UGGUGCGGAUGCCACGACAUCU
1
11
1-1:11
12
ANTIwhiteExon3.fa 513
CGGGAUGCGACUGCUCAAUGGC
1
12
1-1:12
13
White Exon3 7
AGUUCGCCCGGAUAGGCCACGC
1
13
1-1:13
14
White Exon3 135
CGUCCACAGGUUGGCCAUUGAG
1
14
1-1:14
15
White Exon3 263
CUGCCGAUAGGUCAGAUGUCGU
1
15
1-1:15
16
White Exon3 391
GAGGCGAAUGCCAGACGCUUCC
1
16
1-1:16
17
White Exon3 519
UCAGGAUGACGGUCUUGCCCUU
1
17
1-1:17
18
ANTIwhiteIntron2.fa 13
AAUAAAGGGUCCAAUUACCAAU
1
18
1-1:18
19
White intron3 31
GUUGAGAUGUUAUGUAAUACUA
1
19
1-1:19
20
ANTIwhiteAfter3.fa 75
CUGUGACACAUACUUUCUGGGC
1
20
1-1:20
3968 246 Kbytes .Supplementary data files not provided
