IFR140 - Ouest Genopole Transcriptome plateform, UMR6061, UMR GA, Rennes
Manufacture protocol
Probes were suspended in 3X SSC/betaine and printed using MicroGrid II (Biorobotics) arrayer and arrayer software version 2.2.2.8
Support
glass
Coating
amino-silane
Description
This set includes 195 differential display cDNA products from lean and fat chickens, 128 metabolism specific cDNAs, 2 fluorescent oligonucleotide (TagF and TagR, Eurogentec) control probes and 3 ready-to-spot PCR products from Arabidopsis thaliana (corresponding to RUBISCO activase (RCA), root cap 1 (RCP1) and papain-type cysteine endopeptidase (XCP2), Stratagene) at 3 different concentrations (10, 40 and 80 ng/µl). We spotted all the probes in triplicate, except control probes in duplicate, resulting in 1728 spots.