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Platform GPL3234 Query DataSets for GPL3234
Status Public on Feb 08, 2006
Title Invitrogen Yeast Protoarray
Technology type spotted peptide or protein
Distribution commercial
Organism Saccharomyces cerevisiae
Manufacturer Invitrogen
Manufacture protocol The yeast proteome collection was derived from the yeast clone collection of 5800 yeast ORFs. The identity of each clone was verified using 5' end sequencing. Expression of glutathione-S-transferase (GST)-tagged protein by each clone was tested using Western blotting and detection with an anti-GST antibody. The 4088 clones that passed both quality control measures were purified using high-throughput affinity chromatography as previously described. A contact-type printer (Omnigrid, Genomic Solutions) equipped with 48 matched quill-type pins was used to deposit each of 4,088 purified yeast proteins along with a set of control elements in duplicate spots on 1" x 3" glass slides. The printing of these arrays was carried out in a cold room under dust-free conditions in order to preserve the integrity of both samples and printed microarrays. Before releasing these protein microarrays for use, each lot of slides is subjected to a rigorous quality control procedure, including a gross visual inspection of all the printed slides for imperfections. The second step consists of a more detailed characterization of each spot on the array. Since each of the proteins is tagged with GST, this QC procedure is accomplished by using an antibody detection protocol specific for GST. This procedure measures the variability in spot morphology, the number of missing spots, the presence of control spots, and the amount of protein deposited in each spot.
Catalog number PA0121011
Support glass
Coating nitrocellulose
 
Description The Yeast ProtoArray™ high-density functional protein microarray is the first microarray product that enables rapid elucidation of proteinprotein interactions on a proteome scale. The Yeast ProtoArray™ contains 4,088 S. cerevisiae open reading frames (ORFs) expressed as 5'-GST fusions, purified and spotted in duplicate on a 1 inch x 3 inch nitrocellulose-coated slide (Figure 1).With the Yeast ProtoArray™ PPI Kit, you can identify novel protein interactions in a single day, instead of the several weeks required using alternatives such as yeast two-hybrid systems. In addition, protein interactions can be easily assayed under different conditions to better understand interaction dynamics.
 
Web link http://www.invitrogen.com/protoarray
Contributor(s) Hesselberth JR, Miller JP, Michaud GA
Citation(s) 11474067
Submission date Dec 05, 2005
Last update date Feb 08, 2006
Contact name Jay R. Hesselberth
E-mail(s) jay.hesselberth@cuanschutz.edu
Organization name University of Colorado School of Medicine
Department Biochemistry and Molecular Gentetics
Lab Jay Hesselberth
Street address 12801 E 17TH AVE
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
 
Samples (24) GSM86382, GSM86383, GSM86384, GSM86385, GSM86386, GSM86387 
Series (1)
GSE3758 Interactions between Saccharomyces cerevisiae WW domains and proteins discovered using protein microarrays

Data table header descriptions
ID
ORF Saccharomyces cerevisiae systematic gene name
SP_ACC Swissprot accession number
SPOT_ID Name of control printed on protein microarray

Data table
ID ORF SP_ACC SPOT_ID
062004_B01R01C01 --AlexaAntiMouseAb
062004_B01R01C02 --AlexaAntiMouseAb
062004_B01R01C03 --Control15
062004_B01R01C04 --Control15
062004_B01R01C05 --AntiBiotinAb
062004_B01R01C06 --AntiBiotinAb
062004_B01R01C07 --BSA1
062004_B01R01C08 --BSA1
062004_B01R01C09 --BSA2
062004_B01R01C10 --BSA2
062004_B01R01C11 --BSA3
062004_B01R01C12 --BSA3
062004_B01R01C13 --BSA4
062004_B01R01C14 --BSA4
062004_B01R01C15 --BSA5
062004_B01R01C16 --BSA5
062004_B01R02C01 --Control7
062004_B01R02C02 --Control7
062004_B01R02C03 --Control3
062004_B01R02C04 --Control3

Total number of rows: 12288

Table truncated, full table size 375 Kbytes.






Download family Format
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Supplementary file Size Download File type/resource
GPL3234.gal.gz 100.2 Kb (ftp)(http) GAL

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