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Platform GPL10535 Query DataSets for GPL10535
Status Public on Jun 15, 2010
Title Phalanx Escherichia coli 0.155 K Custom Small RNA Array
Technology type spotted oligonucleotide
Distribution custom-commercial
Organism Escherichia coli
Manufacturer Phalanx Biotech Group
Manufacture protocol The sequence of sRNAs and control genes were selected from Ecocyc and Ecogene Database. All of the 50 mer oligonucleotide sequences were design and determined by by ArrayDesign 4.2. Each of the probes was a perfect match to the corresponding E.coli chromosomal sequences. The 155 Probes small RNA array was made by Phalanx Biotech Group. The pre-synthesized DNA oligonucleotides with 5’ amine modification (Biosearch, CA, USA) were spotted and fixed onto the 1’x3’ standard glass by amide formation. To ensure the accuracy, each tri-spot was arranged in a stagger format to form a 9 replication in an 8.7mm*21.1mm printing area. Each glass slide contained 2 such printing areas for any experimental pair.
See http://www.phalanxbiotech.com/ for updated protocols
Support glass
 
Description Phalanx Escherichia coli Custom Small RNA Array is 1 in. X 3 in. (25 mm x 75 mm) standard-format glass slide. Probe is spotted onto the array in a highly consistent manner using an innovative non-contact spotting technology adapted and perfected by Phalanx Biotech for microarray manufacturing.
 
Web link http://www.phalanxbiotech.com/
Contributor(s) Yang C, Ju Y, Huang H, Huang H, Tseng C
Submission date Jun 13, 2010
Last update date Jun 17, 2010
Contact name Chi-Dung Yang
E-mail(s) eden1212.bt92g@nctu.edu.tw
Organization name National Chiao Tung University
Department Department of Biological Science and Technology
Lab Applied Microbiology and Bioengineering Lab
Street address No 75, St. Po-Ai
City Hsin-Chu
ZIP/Postal code 30050
Country Taiwan
 

Data table header descriptions
ID
Gene_name
SEQUENCE probe sequence
ORGANISM
GENE
SPOT_ID

Data table
ID Gene_name SEQUENCE ORGANISM GENE SPOT_ID
H000017 TUB9 AATATCAAGATGCTACAGTCGGTGAAGAGGAGTACGAGGAGGACGAAGAA Arabidopsis thaliana --control
H000018 kinasin TGTTCTCAACCTAATAGATCTTGCTGGAAGCGAGAGGCTATCGAAGAGTG Arabidopsis thaliana --control
H000019 lamine TTCAATGGTCACAGGTCTCGCTCAATTCATGATTCCGGTATCTCTATTCG Arabidopsis thaliana --control
H000020 AT1G TTTGACAATGAAGATGGTGAAGAATCAGACAAGCAAACAGAGAAAGCATA Arabidopsis thaliana --control
H000021 dynesine AAGTTTGTAAATAGGCCCAAGCTAAGAGAGGAAGTTGATTTCAATGATGT Arabidopsis thaliana --control
I000001 hyaE CTTTAGTGTTTACCGGCGGAAACTATCGCGGCGTGCTGAATGGTATTCAC Escherichia coli hyaE
I000002 yebK GCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGA Escherichia coli yebK
I000003 menB CCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCG Escherichia coli menB
I000004 yhbE ATTGGTCATCGTATTTGGGGCGGATTACGTAAGCATACAACGGTGGTATC Escherichia coli yhbE
I000005 polA CGCCAGAGGATTATGTGATTGTCTCAGCGGACTACTCGCAGATTGAACTG Escherichia coli polA
I000006 dnaK CGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAA Escherichia coli dnaK
I000007 gyrA ATGAAATCGCTCCGGAAGTGGACGTTGACGACGAGCCAGAAGAAGAATAA Escherichia coli gyrA
I000008 ftsL CTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAAA Escherichia coli ftsL
I000009 metV CAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATC Escherichia coli metV
I000010 dut TTTGTTCCGGTAGTACAGGCTGAATTTAATCTGGTGGAAGATTTCGACGC Escherichia coli dut
I000011 motB GTATTCCCAACCGTATTAGCCTTTCAGGTCATACCGATGATTTCCCCTAC Escherichia coli motB
I000012 yihA TGAAAGATTTGGATCAGCAGATGATTGAGTGGGCGGTAGACAGCAATATC Escherichia coli yihA
I000013 yjgF TAAAACTACCGTGTTTGTAAAAGATCTGAACGACTTCGCAACCGTAAACG Escherichia coli yjgF
I000014 serU GAAAACCGGAGTAGGGGCAACTCTACCGGGGGTTCAAATCCCCCTCTCTC Escherichia coli serU
I000015 serV TCCCCTGCTAAGGGAGTATGCGGTCAAAAGCTGCATCCGGGGTTCGAATC Escherichia coli serV

Total number of rows: 155

Table truncated, full table size 12 Kbytes.




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