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Status
Public on Jun 09, 2010
Title
Rosetta human miRNA qPCR array
Technology type
RT-PCR
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
Rosetta Inpharmatics
Manufacture protocol
LNA-containing primers were designed as in: Raymond CK, Roberts BS, Garrett-Engele P, Lim LP, Johnson JM. (2005) Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs. RNA 11:1737-44. Oligonucleotides were purchased from Proligo LLC.
Submission date
May 22, 2010
Last update date
Jun 09, 2010
Contact name
Julja Burchard
Organization name
Merck & Co., Inc.
Department
Computational & Systems Biology
Street address
33 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02176
Country
USA
Samples (192)
GSM548041 , GSM548042 , GSM548043 , GSM548044 , GSM548045 , GSM548046
GSM548047 ,
GSM548048 ,
GSM548049 ,
GSM548050 ,
GSM548051 ,
GSM548052 ,
GSM548053 ,
GSM548054 ,
GSM548055 ,
GSM548056 ,
GSM548057 ,
GSM548058 ,
GSM548059 ,
GSM548060 ,
GSM548061 ,
GSM548062 ,
GSM548063 ,
GSM548064 ,
GSM548065 ,
GSM548066 ,
GSM548067 ,
GSM548068 ,
GSM548069 ,
GSM548070 ,
GSM548071 ,
GSM548072 ,
GSM548073 ,
GSM548074 ,
GSM548077 ,
GSM548078 ,
GSM548079 ,
GSM548080 ,
GSM548081 ,
GSM548082 ,
GSM548083 ,
GSM548084 ,
GSM548085 ,
GSM548086 ,
GSM548087 ,
GSM548088 ,
GSM548089 ,
GSM548090 ,
GSM548091 ,
GSM548092 ,
GSM548093 ,
GSM548094 ,
GSM548095 ,
GSM548096 ,
GSM548097 ,
GSM548098 ,
GSM548099 ,
GSM548100 ,
GSM548101 ,
GSM548102 ,
GSM548103 ,
GSM548104 ,
GSM548105 ,
GSM548106 ,
GSM548107 ,
GSM548108 ,
GSM548109 ,
GSM548110 ,
GSM548111 ,
GSM548112 ,
GSM548113 ,
GSM548114 ,
GSM548115 ,
GSM548116 ,
GSM548117 ,
GSM548118 ,
GSM548119 ,
GSM548120 ,
GSM548121 ,
GSM548122 ,
GSM548123 ,
GSM548124 ,
GSM548125 ,
GSM548126 ,
GSM548127 ,
GSM548128 ,
GSM548129 ,
GSM548130 ,
GSM548131 ,
GSM548132 ,
GSM548133 ,
GSM548134 ,
GSM548135 ,
GSM548136 ,
GSM548137 ,
GSM548138 ,
GSM548139 ,
GSM548140 ,
GSM548141 ,
GSM548142 ,
GSM548143 ,
GSM548144 ,
GSM548145 ,
GSM548146 ,
GSM548147 ,
GSM548148 ,
GSM548149 ,
GSM548150 ,
GSM548151 ,
GSM548152 ,
GSM548153 ,
GSM548154 ,
GSM548155 ,
GSM548156 ,
GSM548157 ,
GSM548158 ,
GSM548159 ,
GSM548160 ,
GSM548161 ,
GSM548162 ,
GSM548163 ,
GSM548164 ,
GSM548165 ,
GSM548166 ,
GSM548167 ,
GSM548168 ,
GSM548169 ,
GSM548170 ,
GSM548171 ,
GSM548172 ,
GSM548173 ,
GSM548174 ,
GSM548175 ,
GSM548176 ,
GSM548177 ,
GSM548178 ,
GSM548179 ,
GSM548180 ,
GSM548181 ,
GSM548182 ,
GSM548183 ,
GSM548184 ,
GSM548185 ,
GSM548186 ,
GSM548187 ,
GSM548188 ,
GSM548189 ,
GSM548190 ,
GSM548191 ,
GSM548192 ,
GSM548193 ,
GSM548194 ,
GSM548195 ,
GSM548196 ,
GSM548197 ,
GSM548198 ,
GSM548199 ,
GSM548200 ,
GSM548201 ,
GSM548202 ,
GSM548203 ,
GSM548204 ,
GSM548205 ,
GSM548206 ,
GSM548207 ,
GSM548208 ,
GSM548209 ,
GSM548210 ,
GSM548211 ,
GSM548212 ,
GSM548213 ,
GSM548214 ,
GSM548215 ,
GSM548216 ,
GSM548217 ,
GSM548218 ,
GSM548219 ,
GSM548220 ,
GSM548221 ,
GSM548222 ,
GSM548223 ,
GSM548224 ,
GSM548225 ,
GSM548226 ,
GSM548227 ,
GSM548228 ,
GSM548229 ,
GSM548230 ,
GSM548231 ,
GSM548232 ,
GSM548338 ,
GSM548339 Series (1)
GSE22058 miR-122 as a Regulator of Mitochondrial Metabolic Gene Network in Hepatocellular Carcinoma
Data table header descriptions ID Unique numeric identifier for each miRNA qPCR assay.
miRNA_ID microRNA identifier detected by the particular qPCR assay
SEQUENCE active strand sequence of the microRNA detected by the qPCR assay
PRIMER1_OLIGO sequence of the first qPCR primer
PRIMER2_OLIGO sequence of the second qPCR primer; note that LNA-based residues are marked by '+' adjacent to the nucleotide-base code.
Data table ID miRNA_ID SEQUENCE PRIMER1_OLIGO PRIMER2_OLIGO 10007626745
hsa-let-7a UGAGGUAGUAGGUUGUAUAGUU
CATGATCAGCTGGGCCAAGAAACTATAC
T+GA+GGTAGTAGGTTG
10007626746
hsa-let-7b UGAGGUAGUAGGUUGUGUGGUU
CATGATCAGCTGGGCCAAGAAACCACAC
T+GA+GGTAGTAGGTTG
10007626747
hsa-let-7c UGAGGUAGUAGGUUGUAUGGUU
CATGATCAGCTGGGCCAAGAAACCATAC
T+GA+GGTAGTAGGTTG
10007626748
hsa-let-7d AGAGGUAGUAGGUUGCAUAGU
CATGATCAGCTGGGCCAAGAACTATGCA
A+GA+GGTAGTAGGTTG
10007626749
hsa-let-7e UGAGGUAGGAGGUUGUAUAGU
CATGATCAGCTGGGCCAAGAACTATACA
T+GA+GGTAGGAGGTTG
10007626750
hsa-let-7f UGAGGUAGUAGAUUGUAUAGUU
CATGATCAGCTGGGCCAAGAAACTATAC
T+GA+GGTAGTAGATTG
10007626751
hsa-let-7g UGAGGUAGUAGUUUGUACAGU
CATGATCAGCTGGGCCAAGAACTGTACA
T+GA+GGTAGTAGTTTG
10007626752
hsa-let-7i UGAGGUAGUAGUUUGUGCUGU
CATGATCAGCTGGGCCAAGAACAGCACA
T+GA+GGTAGTAGTTTG
10007626753
hsa-mir-1 UGGAAUGUAAAGAAGUAUGUA
CATGATCAGCTGGGCCAAGATACATACTTC
T+G+GAA+TG+TAAAGAAGT
10007626754
hsa-mir-100 AACCCGUAGAUCCGAACUUGUG
CATGATCAGCTGGGCCAAGACACAAGTTCG
A+AC+CCGTAGATCCG
10007626755
hsa-mir-101 UACAGUACUGUGAUAACUGAAG
CATGATCAGCTGGGCCAAGACTTCAGTTAT
TA+CAG+TACTGTGATAACT
10007626756
hsa-mir-103 AGCAGCAUUGUACAGGGCUAUGA
CATGATCAGCTGGGCCAAGATCATAGCCCT
A+GC+AGCATTGTACA
10007626757
hsa-mir-105 UCAAAUGCUCAGACUCCUGU
CATGATCAGCTGGGCCAAGAACAGGAGTCT
T+CAAA+TGCTCAGACT
10007626758
hsa-mir-106a AAAAGUGCUUACAGUGCAGGUAGC
CATGATCAGCTGGGCCAAGAGCTACCTGCA
AAA+AG+TGCTTACAGTG
10007626759
hsa-mir-106b UAAAGUGCUGACAGUGCAGAU
CATGATCAGCTGGGCCAAGAATCTGCACTG
T+AAAG+TGCTGACAGT
10007626760
hsa-mir-107 AGCAGCAUUGUACAGGGCUAUCA
CATGATCAGCTGGGCCAAGATGATAGCC
A+GC+AGCATTGTACAG
10007626761
hsa-mir-10a UACCCUGUAGAUCCGAAUUUGUG
CATGATCAGCTGGGCCAAGACACAAATTCG
T+AC+CCTGTAGATCCG
10007626762
hsa-mir-10b UACCCUGUAGAACCGAAUUUGU
CATGATCAGCTGGGCCAAGAACAAATTCGGT
TA+CCC+TGT+AGAACCGA
10007626763
hsa-mir-122a UGGAGUGUGACAAUGGUGUUUGU
CATGATCAGCTGGGCCAAGAACAAACACCA
T+GG+AGTGTGACAAT
10007626764
hsa-mir-124a UUAAGGCACGCGGUGAAUGCCA
CATGATCAGCTGGGCCAAGATGGCATTCAC
T+TA+AGGCACGCGGT
220 20 Kbytes .Supplementary data files not provided
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