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Platform GPL10020 Query DataSets for GPL10020
Status Public on Jul 24, 2010
Title Roseburia inulinivorans 5K array
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Roseburia inulinivorans
Manufacturer Rowett Institute of Nutrition and Health
Manufacture protocol This array has been made from random genomic DNA fragments from R. inulinivorans A2-194. A total of 5500 individual colonies were picked from the library using a robotic colony picker and these were amplified by PCR using primers M13 forward and reverse. Approximately 5000 clones containing single inserts of 500 to 2500bp were identified and used for the array. Clones have been saved as glycerol stocks.
Support glass
Coating aminosilane
 
Description The array has been printed in 48 blocks (4 columns and 12 rows), each block contains 324 features; 18 columns with 6 rows in triplicate at 0.20mm spacing. Mixed Arabidopsis thaliana cDNAs have been printed as landmarks in the top left corners of blocks 1,1; 1,4; 4,1; 4,4; 5,1; 5,4; 8,1; 8,4; 9,1; 9,4; 12,1 and 12,4.
 
Submission date Feb 08, 2010
Last update date Jul 24, 2010
Contact name Gill Campbell
E-mail(s) G.Campbell@abdn.ac.uk
Phone +44 (0) 1224 716627
Fax +44 (0) 1224 716647
URL http://www.rowett.ac.uk
Organization name University of Aberdeen
Department Rowett Institute of Nutrition and Health
Street address Greenburn Road
City Aberdeen
ZIP/Postal code AB21 9SB
Country United Kingdom
 
Samples (4) GSM553706, GSM553707, GSM553708, GSM553709
Series (1)
GSE22245 Differential gene expression in Roseburia inulinivorans grown on inulin and starch

Data table header descriptions
ID
MetaColumn Column number of the block within the array
MetaRow Row number of the block within the array
Column Spot column within the block
Row1 Spot row within the block for first replicate
Row2 Spot row within the block for second replicate
Row3 Spot row within the block for third replicate
BioSequence type Type of DNA sequence
Template Clone ID (culture plate and well position)
Reporter Identifier Clone ID (spot plate and well position)
Reporter usage Type of reporter
predicted function
GB_ACC
SPOT_ID

Data table
ID MetaColumn MetaRow Column Row1 Row2 Row3 BioSequence type Template Reporter Identifier Reporter usage predicted function GB_ACC SPOT_ID
13I20 1 1 1 1 7 13 PCR_amplicon relocated1 13I20 Landmark mixed_A.thaliana_PCR_spikes CONTROL
13A4 1 1 1 2 8 14 PCR_amplicon 13A4 13A4 Experimental unknown unknown
12I8 1 1 1 3 9 15 PCR_amplicon 9I8 12I8 Experimental unknown unknown
11A16 1 1 1 4 10 16 PCR_amplicon 11A16 11A16 Experimental unknown unknown
10I20 1 1 1 5 11 17 PCR_amplicon 10I20 10I20 Experimental unknown unknown
10A4 1 1 1 6 12 18 PCR_amplicon 10A4 10A4 Experimental unknown unknown
13M20 1 1 2 1 7 13 PCR_amplicon 13M20 13M20 Experimental unknown unknown
13E04 1 1 2 2 8 14 PCR_amplicon 13E04 13E04 Experimental unknown unknown
12M8 1 1 2 3 9 15 PCR_amplicon 9M8 12M8 Experimental unknown unknown
11E16 1 1 2 4 10 16 PCR_amplicon 11E16 11E16 Experimental unknown unknown
10M20 1 1 2 5 11 17 PCR_amplicon 10M20 10M20 Experimental unknown unknown
10E4 1 1 2 6 12 18 PCR_amplicon 10E4 10E4 Experimental unknown unknown
13A24 1 1 3 1 7 13 PCR_amplicon 13A24 13A24 Experimental unknown unknown
13I4 1 1 3 2 8 14 PCR_amplicon 13I4 13I4 Experimental unknown unknown
12A12 1 1 3 3 9 15 PCR_amplicon 9A12 12A12 Experimental unknown unknown
11I16 1 1 3 4 10 16 PCR_amplicon 11I16 11I16 Experimental unknown unknown
10A24 1 1 3 5 11 17 PCR_amplicon 10A24 10A24 Experimental unknown unknown
10I4 1 1 3 6 12 18 PCR_amplicon 10I4 10I4 Experimental unknown unknown
13E24 1 1 4 1 7 13 PCR_amplicon 3D22 13E24 Experimental unknown unknown
13M4 1 1 4 2 8 14 PCR_amplicon 13M4 13M4 Experimental unknown unknown

Total number of rows: 4992

Table truncated, full table size 352 Kbytes.




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