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Series GSE69761 Query DataSets for GSE69761
Status Public on Jun 11, 2015
Title Fluidigm C1 + Illumina HiSeq quantitative whole transcriptome analysis of unsorted population of E16.5 lung cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We used microfluidic single cell RNA-seq on mixed e16.5 mouse lung cells in order to determine the potential cell types present based on differential transcriptional profiles of the entire population using minimal cell selection bias.
Overall design whole lung mouse e16.5 cells were pooled and loaded onto the Fluidigm C1 device. microwells that contained intact single cells were recorded, the labchip was processed for the generation of cDNA from each cell, and cDNA generated from each accepted well was used to generate amplified and barcoded DNA library that was loaded into an Iluumina HiSeq machine for HTS analysis.
Contributor(s) Dexheimer P, Aronow B, Whitsett J, Potter S, Brunskill E, Shannon J, Xu Y, Guo M, Du Y
Citation(s) 26130332, 26600239
Submission date Jun 10, 2015
Last update date Oct 09, 2019
Contact name Yan Xu
Organization name Cicinnati children's Hospital Medical Center
Department Pulmonary Biology
Street address 3333 Burnet Avenue
City Cincinnati
State/province Ohio
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (148)
GSM1708343 Cell_1
GSM1708344 Cell_10
GSM1708345 Cell_11
BioProject PRJNA286698
SRA SRP059344

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69761_36188g-148c-fpkm.txt.gz 4.3 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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