NCBI Danio rerio Annotation Release 105

The RefSeq genome records for Danio rerio were annotated by the NCBI Eukaryotic Genome Annotation Pipeline, an automated pipeline that annotates genes, transcripts and proteins on draft and finished genome assemblies. This report presents statistics on the annotation products, the input data used in the pipeline and intermediate alignment results.

The annotation products are available in the sequence databases and on the FTP site.

This report provides:

For more information on the annotation process, please visit the NCBI Eukaryotic Genome Annotation Pipeline page.


Annotation Release information

This annotation should be referred to as NCBI Danio rerio Annotation Release 105

Annotation release ID: 105
Date of Entrez queries for transcripts and proteins: Jun 20 2016
Date of submission of annotation to the public databases: Jun 24 2016
Software version: 7.1

Assemblies

The following assemblies were included in this annotation run:
Assembly nameAssembly accessionSubmitterAssembly dateReference/AlternateAssembly content
GRCz10GCF_000002035.5Genome Reference Consortium09-02-2014Reference26 assembled chromosomes; unplaced scaffolds

Gene and feature statistics

Counts and length of annotated features are provided below for each assembly.

Feature counts

FeatureGRCz10
Genes and pseudogenes help42,154
  protein-coding26,373
  non-coding15,445
  pseudogenes336
  genes with variants10,860
mRNAs46,440
  fully-supported44,617
  with > 5% ab initio help815
  partial2,005
  with filled gap(s) help583
  known RefSeq (NM_) help15,265
  model RefSeq (XM_)31,175
Other RNAs help18,595
  fully-supported9,498
  with > 5% ab initio help0
  partial12
  with filled gap(s) help5
  known RefSeq (NR_) help439
  model RefSeq (XR_) help9,082
CDSs46,551
  fully-supported44,617
  with > 5% ab initio help940
  partial1,472
  with major correction(s) help729
  known RefSeq (NP_) help15,263
  model RefSeq (XP_) help31,175

Detailed reports

Alignment of the annotated proteins to a set of high-quality proteins

The final set of annotated proteins was searched with BLASTP against the UniProtKB/Swiss-Prot curated proteins, using the annotated proteins as the query and the high-quality proteins as the target. Out of 26272 coding genes, 23792 genes had a protein with an alignment covering 50% or more of the query and 12566 had an alignment covering 95% or more of the query.

Definition of query and target coverage. The query coverage is the percentage of the annotated protein length that is included in the alignment. The target coverage is the percentage of the target length that is included in the alignment.

Below is a cumulative graph displaying the number of genes with alignments above a given query or target coverage threshold. For comparison, corresponding statistics for other organisms annotated by the NCBI eukaryotic annotation pipeline were added to the graph.

Query: annotated proteins
Target: UniProtKB/Swiss-Prot curated proteins

Masking of genomic sequence

Transcript and protein alignments are performed on the repeat-masked genome. Below are the percentages of genomic sequence masked by WindowMasker and RepeatMasker for each assembly. RepeatMasker results are only used for organisms for which a comprehensive repeat library is available.

For this annotation run, transcripts and proteins were aligned to the genome masked with WindowMasker only.
Assembly nameAssembly accession% Masked with RepeatMasker% Masked with WindowMasker
GRCz10GCF_000002035.558.40%49.09%

Transcript and protein alignments

The annotation pipeline relies heavily on alignments of experimental evidence for gene prediction. Below are the sets of transcripts and proteins that were retrieved from Entrez, aligned to the genome by Splign or ProSplign and passed to Gnomon, NCBI's gene prediction software.

Depending on the other evidence available, long 454 reads (with average length above 250 nt) may be aligned as traditional evidence and reported in the Transcript alignments section or aligned with RNA-Seq reads and reported in the RNA-Seq alignments section.

Transcript alignments

RefSeq transcript alignment quality report

The known RefSeq transcripts (NM_ and NR_ accessions) are a set of hiqh-quality transcripts maintained by the RefSeq group at NCBI. Alignment statistics for this group of transcripts, such as percent and number of sequences not aligning at all, percent best alignments split between multiple scaffolds, and percent alignments not covering the full CDS are indicative of the genome quality and are provided below.

GRCz10
Primary Assembly
Number of sequences retrieved from Entrez16,040
Number (%) of sequences not aligning32 (0.20%)
Number (%) of sequences with multiple best alignments (split genes)208 (1.30%)
Number (%) of sequences with CDS coverage < 95% help561 (3.61%)

RNA-Seq alignments

The following RNA-Seq reads from the Sequence Read Archive were also used for gene prediction:

  Hide alignments statistics, by sample (SAME, SAMN, SAMD, DRS)
  Show alignments statistics, by run (ERR, SRR, DRR)

Protein alignments

Comparison of the current and previous annotations

The annotation produced for this release (105) was compared to the annotation in the previous release (104) for each assembly annotated in both releases. Scores for current and previous gene and transcript features were calculated based on overlap in exon sequence and matches in exon boundaries. Pairs of current and previous features were categorized based on these scores, whether they are reciprocal best matches, and changes in attributes (gene biotype, completeness, etc.). If the assembly was updated between the two releases, alignments between the current and the previous assembly were used to match the current and previous gene and transcript features in mapped regions.

The table below summarizes the changes in the gene set for each assembly as a percent of the number of genes in the current annotation release, and provides links to the details of the comparison in tabular format and in a Genome Workbench project.

GRCz10 (Current) to GRCz10 (Previous)
Identical help22%
Minor changes help38%
Major changes help9%
New help10%
Deprecated help5%
Other help21%
Download the reporttabular, Genome Workbench

References

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