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    NCK2 NCK adaptor protein 2 [ Homo sapiens (human) ]

    Gene ID: 8440, updated on 22-Jan-2023

    Summary

    Official Symbol
    NCK2provided by HGNC
    Official Full Name
    NCK adaptor protein 2provided by HGNC
    Primary source
    HGNC:HGNC:7665
    See related
    Ensembl:ENSG00000071051 MIM:604930; AllianceGenome:HGNC:7665
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    GRB4; NCKbeta
    Summary
    This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in duodenum (RPKM 14.7), spleen (RPKM 14.6) and 25 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See NCK2 in Genome Data Viewer
    Location:
    2q12.2
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    110 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (105744453..105894272)
    110 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (106206144..106355582)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (106361369..106510728)

    Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene long intergenic non-protein coding RNA 2946 Neighboring gene uncharacterized LOC107985929 Neighboring gene uncharacterized LOC105373530 Neighboring gene uncharacterized LOC105373531 Neighboring gene uncharacterized LOC124906055

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    EBI GWAS Catalog

    Description
    Genome-wide meta-analysis of systolic blood pressure in children with sickle cell disease.
    EBI GWAS Catalog
    NCK2 is significantly associated with opiates addiction in African-origin men.
    EBI GWAS Catalog

    HIV-1 interactions

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env HIV-1 gp120 downregulates the expression of NCK adaptor protein 2 (NCK2) in human B cells PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables cytoskeletal anchor activity NAS
    Non-traceable Author Statement
    more info
    PubMed 
    enables phosphotyrosine residue binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein-containing complex binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables scaffold protein binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables signaling adaptor activity NAS
    Non-traceable Author Statement
    more info
    PubMed 
    enables signaling receptor complex adaptor activity NAS
    Non-traceable Author Statement
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in T cell activation NAS
    Non-traceable Author Statement
    more info
    PubMed 
    involved_in actin filament organization IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in dendritic spine development IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in ephrin receptor signaling pathway IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in epidermal growth factor receptor signaling pathway TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in immunological synapse formation IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in lamellipodium assembly IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in negative regulation of PERK-mediated unfolded protein response IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in negative regulation of cell population proliferation TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation of peptidyl-serine phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in positive regulation of T cell proliferation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of actin filament polymerization IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of translation in response to endoplasmic reticulum stress IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in regulation of epidermal growth factor-activated receptor activity TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in signal complex assembly NAS
    Non-traceable Author Statement
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytoplasm NAS
    Non-traceable Author Statement
    more info
    PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in postsynaptic density IEA
    Inferred from Electronic Annotation
    more info
     
    located_in vesicle membrane IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    cytoplasmic protein NCK2
    Names
    SH2/SH3 adaptor protein NCK-beta
    growth factor receptor-bound protein 4
    noncatalytic region of tyrosine kinase, beta

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001004720.3NP_001004720.1  cytoplasmic protein NCK2 isoform A

      See identical proteins and their annotated locations for NP_001004720.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (A).
      Source sequence(s)
      AC009505, BC000103, DB456958
      Consensus CDS
      CCDS33266.1
      UniProtKB/Swiss-Prot
      O43639, Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
      Related
      ENSP00000377018.2, ENST00000393349.2
      Conserved Domains (4) summary
      cd10409
      Location:283380
      SH2_Nck2; Src homology 2 (SH2) domain found in Nck
      cd11899
      Location:259
      SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
      cd11902
      Location:114168
      SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
      cd11903
      Location:198256
      SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
    2. NM_001004722.4NP_001004722.1  cytoplasmic protein NCK2 isoform B

      See identical proteins and their annotated locations for NP_001004722.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) lacks an exon, which results in a frameshift, compared to variant 1. The resulting protein (isoform B) is shorter and has a distinct C-terminus, compared to isoform A.
      Source sequence(s)
      AC009505, AC010978, BG480643
      UniProtKB/TrEMBL
      E7ERP6
      Related
      ENSP00000410428.2, ENST00000451463.6
      Conserved Domains (1) summary
      cl17036
      Location:259
      SH3; Src Homology 3 domain superfamily
    3. NM_003581.5NP_003572.2  cytoplasmic protein NCK2 isoform A

      See identical proteins and their annotated locations for NP_003572.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (A). Variants 1 and 2 both encode isoform A.
      Source sequence(s)
      AC009505, AC010978, BC007195
      Consensus CDS
      CCDS33266.1
      UniProtKB/Swiss-Prot
      O43639, Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
      Related
      ENSP00000233154.4, ENST00000233154.9
      Conserved Domains (4) summary
      cd10409
      Location:283380
      SH2_Nck2; Src homology 2 (SH2) domain found in Nck
      cd11899
      Location:259
      SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
      cd11902
      Location:114168
      SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
      cd11903
      Location:198256
      SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

      Range
      105744453..105894272
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017005105.2XP_016860594.1  cytoplasmic protein NCK2 isoform X1

      UniProtKB/Swiss-Prot
      O43639, Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
      Conserved Domains (4) summary
      cd10409
      Location:283380
      SH2_Nck2; Src homology 2 (SH2) domain found in Nck
      cd11899
      Location:259
      SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
      cd11902
      Location:114168
      SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
      cd11903
      Location:198256
      SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
    2. XM_017005103.2XP_016860592.1  cytoplasmic protein NCK2 isoform X1

      UniProtKB/Swiss-Prot
      O43639, Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
      Conserved Domains (4) summary
      cd10409
      Location:283380
      SH2_Nck2; Src homology 2 (SH2) domain found in Nck
      cd11899
      Location:259
      SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
      cd11902
      Location:114168
      SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
      cd11903
      Location:198256
      SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
    3. XM_011511991.4XP_011510293.1  cytoplasmic protein NCK2 isoform X1

      See identical proteins and their annotated locations for XP_011510293.1

      UniProtKB/Swiss-Prot
      O43639, Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
      Conserved Domains (4) summary
      cd10409
      Location:283380
      SH2_Nck2; Src homology 2 (SH2) domain found in Nck
      cd11899
      Location:259
      SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
      cd11902
      Location:114168
      SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
      cd11903
      Location:198256
      SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
    4. XM_047446021.1XP_047301977.1  cytoplasmic protein NCK2 isoform X2

      UniProtKB/TrEMBL
      E7ERP6
      Related
      ENSP00000431109.1, ENST00000522586.5
    5. XM_006712797.4XP_006712860.1  cytoplasmic protein NCK2 isoform X1

      See identical proteins and their annotated locations for XP_006712860.1

      UniProtKB/Swiss-Prot
      O43639, Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
      Conserved Domains (4) summary
      cd10409
      Location:283380
      SH2_Nck2; Src homology 2 (SH2) domain found in Nck
      cd11899
      Location:259
      SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
      cd11902
      Location:114168
      SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
      cd11903
      Location:198256
      SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
    6. XM_047446019.1XP_047301975.1  cytoplasmic protein NCK2 isoform X1

      UniProtKB/Swiss-Prot
      Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
    7. XM_017005104.2XP_016860593.1  cytoplasmic protein NCK2 isoform X1

      UniProtKB/Swiss-Prot
      O43639, Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
      Conserved Domains (4) summary
      cd10409
      Location:283380
      SH2_Nck2; Src homology 2 (SH2) domain found in Nck
      cd11899
      Location:259
      SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein
      cd11902
      Location:114168
      SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein
      cd11903
      Location:198256
      SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein
    8. XM_047446020.1XP_047301976.1  cytoplasmic protein NCK2 isoform X1

      UniProtKB/Swiss-Prot
      Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
    9. XM_047446017.1XP_047301973.1  cytoplasmic protein NCK2 isoform X1

      UniProtKB/Swiss-Prot
      Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
    10. XM_047446018.1XP_047301974.1  cytoplasmic protein NCK2 isoform X1

      UniProtKB/Swiss-Prot
      Q9UIC3
      UniProtKB/TrEMBL
      A0A0S2Z4M6
    11. XM_011511992.3XP_011510294.2  cytoplasmic protein NCK2 isoform X2

      UniProtKB/TrEMBL
      E7ERP6
      Conserved Domains (1) summary
      cl17036
      Location:259
      SH3; Src Homology 3 domain superfamily

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060926.1 Alternate T2T-CHM13v2.0

      Range
      106206144..106355582
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)