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    RAD23A RAD23 homolog A, nucleotide excision repair protein [ Homo sapiens (human) ]

    Gene ID: 5886, updated on 26-Sep-2021

    Summary

    Official Symbol
    RAD23Aprovided by HGNC
    Official Full Name
    RAD23 homolog A, nucleotide excision repair proteinprovided by HGNC
    Primary source
    HGNC:HGNC:9812
    See related
    Ensembl:ENSG00000179262 MIM:600061
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    HR23A; HHR23A
    Summary
    The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair. Proteins in this family have a modular domain structure consisting of an ubiquitin-like domain (UbL), ubiquitin-associated domain 1 (UbA1), XPC-binding domain and UbA2. The protein encoded by this gene plays an important role in nucleotide excision repair and also in delivery of polyubiquitinated proteins to the proteasome. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]
    Expression
    Ubiquitous expression in fat (RPKM 50.3), heart (RPKM 47.4) and 25 other tissues See more
    Orthologs
    NEW
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    Genomic context

    See RAD23A in Genome Data Viewer
    Location:
    19p13.13
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    109.20210514 current GRCh38.p13 (GCF_000001405.39) 19 NC_000019.10 (12945846..12953642)
    105.20201022 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (13056676..13064456)

    Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene CRISPRi-FlowFISH-validated RAD23A regulatory element Neighboring gene microRNA 6515 Neighboring gene calreticulin Neighboring gene GADD45G interacting protein 1 Neighboring gene DAN domain BMP antagonist family member 5 Neighboring gene nuclear factor I X Neighboring gene uncharacterized LOC107985286

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    Associated conditions

    Description Tests
    Clozapine-induced agranulocytosis is associated with rare HLA-DQB1 and HLA-B alleles.
    GeneReviews: Not available

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    Knockdown of RAD23 homolog A by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Vpr vpr Binding of HIV-1 Vpr (amino acids 25-77) to the UBA(2) domain of RAD23A (HHR23A) (amino acids 319-363) affects the cell cycle arrest induced by Vpr PubMed
    vpr NMR chemical shift analysis demonstrates that HIV-1 Vpr binds hHR23A through the contact surfaces on the XPCB (residues 232-286) and UBA2 (residues 316-363) domains of hHR23A PubMed
    vpr A di-Ub(K48)-hHR23A-Vpr ternary complex is formed with Lys-48-linked di-ubiquitin binding to the UBA1 domain in the Vpr-hHR23A complex PubMed
    vpr Residues Q249, L255, L259, N266, and N285 in XPCB domain and residues R326, G331, F332, E334, L336, F342, K346, E348, N349, A351, N353, and Q358 in UBA2 domain of hHR23A are involved in the binding to HIV-1 Vpr PubMed
    vpr HIV-1 Vpr promotes cellular protein polyubiquitination via hHR23A. Depletion of hHR23A significantly reduces HIV-1 replication in a Vpr-dependent manner PubMed
    vpr Co-expression of HIV-1 Vpr with HHR23A neutralizes inhibitory effects of HHR23A on p53 transcriptional activity PubMed
    vpr Overexpression of HHR23A causes apoptosis of cells, suggesting that Vpr binding to HHR23A may be involved in Vpr-induced apoptosis PubMed
    vpr Two reports indicate overexpression of HHR23A can inhibit HIV-1 Vpr-induced cell cycle arrest, while a third report indicates Vpr binding to HHR23A does not correlate with the ability of Vpr to induce cell cycle arrest PubMed

    Go to the HIV-1, Human Interaction Database

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    Clone Names

    • MGC111083

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables damaged DNA binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables kinase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables polyubiquitin modification-dependent protein binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    enables polyubiquitin modification-dependent protein binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables proteasome binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables single-stranded DNA binding TAS
    Traceable Author Statement
    more info
    PubMed 
    enables ubiquitin binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    enables ubiquitin-specific protease binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in centriolar satellite IDA
    Inferred from Direct Assay
    more info
     
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
     
    located_in intracellular membrane-bounded organelle IDA
    Inferred from Direct Assay
    more info
     
    is_active_in nucleoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of proteasome complex IEA
    Inferred from Electronic Annotation
    more info
     
    part_of protein-containing complex IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 

    General protein information

    Preferred Names
    UV excision repair protein RAD23 homolog A
    Names
    RAD23, yeast homolog, A

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001270362.2NP_001257291.1  UV excision repair protein RAD23 homolog A isoform 2

      See identical proteins and their annotated locations for NP_001257291.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
      Source sequence(s)
      AI081136, AK289908, BC014026, BP213821
      Consensus CDS
      CCDS59358.1
      UniProtKB/Swiss-Prot
      P54725
      UniProtKB/TrEMBL
      A8K1J3
      Related
      ENSP00000321365.3, ENST00000316856.7
      Conserved Domains (1) summary
      TIGR00601
      Location:3360
      rad23; UV excision repair protein Rad23
    2. NM_001270363.2NP_001257292.1  UV excision repair protein RAD23 homolog A isoform 3

      See identical proteins and their annotated locations for NP_001257292.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1.
      Source sequence(s)
      AC092069, BC014026, BP213821, BX448989
      Consensus CDS
      CCDS59357.1
      UniProtKB/Swiss-Prot
      P54725
      Related
      ENSP00000468674.1, ENST00000592268.5
      Conserved Domains (1) summary
      cl28408
      Location:3306
      UBQ; Ubiquitin homologues
    3. NM_005053.4NP_005044.1  UV excision repair protein RAD23 homolog A isoform 1

      See identical proteins and their annotated locations for NP_005044.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
      Source sequence(s)
      BC014026, BP213821
      Consensus CDS
      CCDS12289.1
      UniProtKB/Swiss-Prot
      P54725
      UniProtKB/TrEMBL
      A0A024R7G8
      Related
      ENSP00000467024.1, ENST00000586534.6
      Conserved Domains (1) summary
      TIGR00601
      Location:3361
      rad23; UV excision repair protein Rad23

    RNA

    1. NR_072976.2 RNA Sequence

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) lacks an alternate internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AI057203, AK293219, BP213821
      Related
      ENST00000593114.5

    RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20210514

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p13 Primary Assembly

    Genomic

    1. NC_000019.10 Reference GRCh38.p13 Primary Assembly

      Range
      12945846..12953642
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_024451631.1XP_024307399.1  UV excision repair protein RAD23 homolog A isoform X1

      Conserved Domains (2) summary
      cl26386
      Location:81378
      DNA_pol3_gamma3; DNA polymerase III subunits gamma and tau domain III
      cl28408
      Location:3228
      UBQ; Ubiquitin homologues
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