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    Aak1 AP2 associated kinase 1 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 500244, updated on 8-Apr-2026
    Official Symbol
    Aak1provided by RGD
    Official Full Name
    AP2 associated kinase 1provided by RGD
    Primary source
    RGD:1305520
    See related
    Ensembl:ENSRNOG00000018317 AllianceGenome:RGD:1305520
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    RGD1563580
    Summary
    Enables AP-2 adaptor complex binding activity and protein serine/threonine kinase activity. Involved in presynaptic endocytosis and regulation of clathrin-dependent endocytosis. Located in cell leading edge and terminal bouton. Part of clathrin complex. Is active in calyx of Held. Orthologous to human AAK1 (AP2 associated kinase 1). [provided by Alliance of Genome Resources, Jul 2025]
    Expression
    Biased expression in Brain (RPKM 315.7), Adrenal (RPKM 93.7) and 9 other tissues See more
    Orthologs
    all
    Try the new Gene page
    Try the new Transcripts and proteins table
    See Aak1 in Genome Data Viewer
    Location:
    4q34
    Exon count:
    23
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 4 NC_086022.1 (120845012..121009170)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 4 NC_051339.1 (119300128..119451834)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 4 NC_005103.4 (118653851..118806796)

    Chromosome 4 - NC_086022.1Genomic Context describing neighboring genes Neighboring gene annexin A4 Neighboring gene small nucleolar RNA SNORA2/SNORA34 family Neighboring gene uncharacterized LOC134486606 Neighboring gene uncharacterized LOC134486605 Neighboring gene ribosomal protein L22, pseudogene 2 Neighboring gene uncharacterized LOC134486608 Neighboring gene uncharacterized LOC120102328 Neighboring gene NFU1 iron-sulfur cluster scaffold Neighboring gene glutamine fructose-6-phosphate transaminase 1

    Go to nucleotide: Graphics FASTA GenBank

    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Related articles in PubMed

    1. miRNA-384-3p alleviates sevoflurane-induced nerve injury by inhibiting Aak1 kinase in neonatal rats. Chen Y, et al. Brain Behav, 2022 Jul. PMID 35726359, Free PMC Article
    2. The adaptor-associated kinase 1, AAK1, is a positive regulator of the Notch pathway. Gupta-Rossi N, et al. J Biol Chem, 2011 May 27. PMID 21464124, Free PMC Article
    3. AAK1 regulates Numb function at an early step in clathrin-mediated endocytosis. Sorensen EB, et al. Traffic, 2008 Sep. PMID 18657069
    4. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Henderson DM, et al. Mol Biol Cell, 2007 Jul. PMID 17494869, Free PMC Article
    5. AAK1-mediated micro2 phosphorylation is stimulated by assembled clathrin. Conner SD, et al. Traffic, 2003 Dec. PMID 14617351

    See all (12) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. miRNA-384-3p alleviates sevoflurane-induced nerve injury by inhibiting Aak1 kinase in neonatal rats.
      Title: miRNA-384-3p alleviates sevoflurane-induced nerve injury by inhibiting Aak1 kinase in neonatal rats.
    2. This study demonistrated that AAK1 contributes to dendrite growth regulation.
      Title: Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.
    3. AAK1 is a relevant target of K252a, that the loss of AAK1 alters ErbB4 trafficking and expression levels, providing evidence for a previously unrecognized role for AAK1 in Nrg1-mediated neurotrophic factor signaling.
      Title: AAK1 identified as an inhibitor of neuregulin-1/ErbB4-dependent neurotrophic factor signaling using integrative chemical genomics and proteomics.
    Submit: New GeneRIF Correction
    Products Interactant Other Gene Complex Source Pubs Description

    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables AP-2 adaptor complex binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables AP-2 adaptor complex binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables AP-2 adaptor complex binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables Notch binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables Notch binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables protein kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine/threonine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein serine/threonine kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in positive regulation of Notch signaling pathway ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of Notch signaling pathway ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in presynaptic endocytosis EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in presynaptic endocytosis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in presynaptic endocytosis ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein phosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein stabilization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein stabilization ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in regulation of clathrin-dependent endocytosis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of clathrin-dependent endocytosis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of clathrin-dependent endocytosis ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of protein localization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of protein localization ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Component Evidence Code Pubs
    is_active_in calyx of Held EXP
    Inferred from Experiment
    more info
    		 PubMed 
    is_active_in calyx of Held IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell leading edge IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of clathrin complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in clathrin-coated pit IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in clathrin-coated pit ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in clathrin-coated vesicle ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in presynapse IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in presynapse IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in presynapse IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in terminal bouton IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Preferred Names
    AP2-associated protein kinase 1
    Names
    adaptor-associated kinase 1
    NP_001166921.1
    XP_006236895.1
    XP_006236896.1
    XP_017448306.1
    XP_017448307.1
    XP_017448308.1
    XP_017448309.1
    XP_017448310.1
    XP_017448311.1
    XP_038964087.1
    XP_038964088.1
    XP_038964089.1
    XP_038964090.1
    XP_063142600.1
    XP_063142601.1
    XP_063142602.1

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001173450.1 → NP_001166921.1  AP2-associated protein kinase 1

      See identical proteins and their annotated locations for NP_001166921.1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000004
      UniProtKB/Swiss-Prot
      P0C1X8
      UniProtKB/TrEMBL
      F1LRI7
      Related
      ENSRNOP00000044665.4, ENSRNOT00000043082.6
      Conserved Domains (2) summary
      smart00220
      Location:47 → 307
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd14037
      Location:42 → 316
      STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086022.1 Reference GRCr8

      Range
      120845012..121009170
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017592818.3 → XP_017448307.1  AP2-associated protein kinase 1 isoform X1

      UniProtKB/TrEMBL
      A0A8I5ZKV0

    2. XM_017592822.3 → XP_017448311.1  AP2-associated protein kinase 1 isoform X10

      UniProtKB/TrEMBL
      A0A8I6A256, A0A8I6GFC9
      Related
      ENSRNOP00000084865.1, ENSRNOT00000118607.2

    3. XM_006236833.5 → XP_006236895.1  AP2-associated protein kinase 1 isoform X12

      See identical proteins and their annotated locations for XP_006236895.1

      UniProtKB/Swiss-Prot
      P0C1X8
      UniProtKB/TrEMBL
      F1LRI7
      Conserved Domains (2) summary
      smart00220
      Location:47 → 307
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd14037
      Location:42 → 316
      STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases

    4. XM_017592817.3 → XP_017448306.1  AP2-associated protein kinase 1 isoform X1

      UniProtKB/TrEMBL
      A0A8I5ZKV0

    5. XM_017592820.3 → XP_017448309.1  AP2-associated protein kinase 1 isoform X4

      UniProtKB/TrEMBL
      A0A8I5ZKV0
      Related
      ENSRNOP00000078415.2, ENSRNOT00000104791.2

    6. XM_039108160.2 → XP_038964088.1  AP2-associated protein kinase 1 isoform X5

      UniProtKB/TrEMBL
      A0A8I5ZKV0
      Conserved Domains (3) summary
      cd14037
      Location:42 → 316
      STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases
      pfam05109
      Location:320 → 423
      Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)
      pfam15282
      Location:982 → 1193
      BMP2K_C; BMP-2-inducible protein kinase C-terminus

    7. XM_039108159.2 → XP_038964087.1  AP2-associated protein kinase 1 isoform X3

      UniProtKB/TrEMBL
      A0A8I5ZKV0
      Conserved Domains (3) summary
      cd14037
      Location:42 → 316
      STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases
      pfam05109
      Location:320 → 423
      Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)
      pfam15282
      Location:1058 → 1269
      BMP2K_C; BMP-2-inducible protein kinase C-terminus

    8. XM_039108161.2 → XP_038964089.1  AP2-associated protein kinase 1 isoform X7

      UniProtKB/TrEMBL
      A0A8I5ZKV0
      Conserved Domains (3) summary
      cd14037
      Location:42 → 316
      STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases
      pfam05109
      Location:320 → 423
      Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)
      pfam15282
      Location:950 → 1161
      BMP2K_C; BMP-2-inducible protein kinase C-terminus

    9. XM_017592819.3 → XP_017448308.1  AP2-associated protein kinase 1 isoform X2

      UniProtKB/TrEMBL
      A0A8I5ZKV0, A0A8I6A6D5
      Related
      ENSRNOP00000088529.2, ENSRNOT00000102896.2

    10. XM_063286530.1 → XP_063142600.1  AP2-associated protein kinase 1 isoform X6

      UniProtKB/TrEMBL
      A0A8I5ZKV0

    11. XM_017592821.3 → XP_017448310.1  AP2-associated protein kinase 1 isoform X8

      UniProtKB/TrEMBL
      A0A8I5ZKV0, A0A8I6ALS6
      Related
      ENSRNOP00000094963.1, ENSRNOT00000096717.2

    12. XM_063286531.1 → XP_063142601.1  AP2-associated protein kinase 1 isoform X9

      UniProtKB/TrEMBL
      A0A8I5ZKV0

    13. XM_039108162.2 → XP_038964090.1  AP2-associated protein kinase 1 isoform X11

      UniProtKB/TrEMBL
      A0A8I6GFC9
      Conserved Domains (3) summary
      cd14037
      Location:42 → 316
      STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases
      pfam05109
      Location:320 → 423
      Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)
      pfam15282
      Location:915 → 1126
      BMP2K_C; BMP-2-inducible protein kinase C-terminus

    14. XM_063286532.1 → XP_063142602.1  AP2-associated protein kinase 1 isoform X14

      UniProtKB/Swiss-Prot
      P0C1X8

    15. XM_006236834.5 → XP_006236896.1  AP2-associated protein kinase 1 isoform X13

      UniProtKB/Swiss-Prot
      P0C1X8
      Conserved Domains (2) summary
      smart00220
      Location:47 → 307
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd14037
      Location:42 → 316
      STKc_NAK_like; Catalytic domain of Numb-Associated Kinase (NAK)-like Serine/Threonine kinases
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P0C1X8.1 GenPept UniProtKB/Swiss-Prot:P0C1X8

    Gene LinkOut

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