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    NONO non-POU domain containing octamer binding [ Homo sapiens (human) ]

    Gene ID: 4841, updated on 8-Jan-2023

    Summary

    Official Symbol
    NONOprovided by HGNC
    Official Full Name
    non-POU domain containing octamer bindingprovided by HGNC
    Primary source
    HGNC:HGNC:7871
    See related
    Ensembl:ENSG00000147140 MIM:300084; AllianceGenome:HGNC:7871
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
    Summary
    This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
    Expression
    Ubiquitous expression in ovary (RPKM 81.7), lymph node (RPKM 64.3) and 25 other tissues See more
    Orthologs
    NEW
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    Genomic context

    See NONO in Genome Data Viewer
    Location:
    Xq13.1
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    110 current GRCh38.p14 (GCF_000001405.40) X NC_000023.11 (71283635..71301168)
    110 current T2T-CHM13v2.0 (GCF_009914755.1) X NC_060947.1 (69717744..69735278)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) X NC_000023.10 (70503485..70521018)

    Chromosome X - NC_000023.11Genomic Context describing neighboring genes Neighboring gene gap junction protein beta 1 Neighboring gene zinc finger MYM-type containing 3 Neighboring gene uncharacterized LOC105373247 Neighboring gene integrin subunit beta 1 binding protein 2 Neighboring gene ZCRB1 pseudogene 1

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    Knockdown of NONO by shRNA enhances HIV-1 replication in both Jurkat cells and primary CD4+ T-cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Nef nef Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Pr55(Gag) gag Knockdown of NONO expression by shRNA enhances both HIV-1 Gag expression in Jurkat cells and the released p24 levels from these cells PubMed
    gag Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Rev rev Co-immunoprecipitation analysis demonstrates that endogenous RBM14 associates with HIV-1 Rev, XPO1, and p54/NRB in cells PubMed
    rev HIV-1 Rev interacting protein, non-POU domain containing, octamer-binding (NONO), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with NONO is increased by RRE PubMed
    Vpr vpr A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies upregulation of non-POU domain containing octamer-binding protein (NONO) expression by HIV-1 Vpr in Vpr transduced macrophages PubMed
    capsid gag Knockdown of NONO expression by shRNA enhances both HIV-1 Gag expression in Jurkat cells and the released p24 levels from these cells PubMed
    retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human non-POU domain containing, octamer-binding (NONO) at amino acid residues 54-55, 274-275, and 277-278 by the HIV-1 protease PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables RNA binding HDA PubMed 
    enables chromatin binding ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in RNA splicing IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in activation of innate immune response IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in circadian rhythm ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in innate immune response IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in mRNA processing IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in negative regulation of DNA-templated transcription ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of DNA-templated transcription IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in regulation of circadian rhythm ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    Component Evidence Code Pubs
    part_of RNA polymerase II transcription regulator complex ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
     
    located_in fibrillar center IDA
    Inferred from Direct Assay
    more info
     
    located_in membrane HDA PubMed 
    located_in nuclear matrix IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nuclear speck IEA
    Inferred from Electronic Annotation
    more info
     
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleus ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in paraspeckles IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    non-POU domain-containing octamer-binding protein
    Names
    54 kDa nuclear RNA- and DNA-binding protein
    55 kDa nuclear protein
    DNA-binding p52/p100 complex, 52 kDa subunit
    non-POU domain-containing octamer (ATGCAAAT) binding protein
    p54(nrb)
    protein phosphatase 1, regulatory subunit 114

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_046742.1 RefSeqGene

      Range
      5444..22977
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001145408.2NP_001138880.1  non-POU domain-containing octamer-binding protein isoform 1

      See identical proteins and their annotated locations for NP_001138880.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
      Source sequence(s)
      BC002364, BC003129, DA116840
      Consensus CDS
      CCDS14410.1
      UniProtKB/Swiss-Prot
      Q15233, Q9BQC5
      UniProtKB/TrEMBL
      A0A0S2Z4Z9
      Related
      ENSP00000410299.2, ENST00000420903.6
      Conserved Domains (3) summary
      cd12946
      Location:219311
      NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
      cd12588
      Location:73143
      RRM1_p54nrb; RNA recognition motif 1 (RRM1) found in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)
      cl17169
      Location:149228
      RRM_SF; RNA recognition motif (RRM) superfamily
    2. NM_001145409.2NP_001138881.1  non-POU domain-containing octamer-binding protein isoform 1

      See identical proteins and their annotated locations for NP_001138881.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).
      Source sequence(s)
      BC002364, BC012141, DA116840
      Consensus CDS
      CCDS14410.1
      UniProtKB/Swiss-Prot
      Q15233, Q9BQC5
      UniProtKB/TrEMBL
      A0A0S2Z4Z9
      Related
      ENSP00000362947.1, ENST00000373841.5
      Conserved Domains (3) summary
      cd12946
      Location:219311
      NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
      cd12588
      Location:73143
      RRM1_p54nrb; RNA recognition motif 1 (RRM1) found in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)
      cl17169
      Location:149228
      RRM_SF; RNA recognition motif (RRM) superfamily
    3. NM_001145410.2NP_001138882.1  non-POU domain-containing octamer-binding protein isoform 2

      See identical proteins and their annotated locations for NP_001138882.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
      Source sequence(s)
      AK297144, BC002364
      Consensus CDS
      CCDS55445.1
      UniProtKB/Swiss-Prot
      Q15233
      Related
      ENSP00000441364.1, ENST00000535149.5
      Conserved Domains (2) summary
      cd12946
      Location:130222
      NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
      cl17169
      Location:60139
      RRM_SF; RNA recognition motif (RRM) superfamily
    4. NM_007363.5NP_031389.3  non-POU domain-containing octamer-binding protein isoform 1

      See identical proteins and their annotated locations for NP_031389.3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).
      Source sequence(s)
      BC002364, BC028299, DA116840
      Consensus CDS
      CCDS14410.1
      UniProtKB/Swiss-Prot
      Q15233, Q9BQC5
      UniProtKB/TrEMBL
      A0A0S2Z4Z9
      Related
      ENSP00000276079.8, ENST00000276079.13
      Conserved Domains (3) summary
      cd12946
      Location:219311
      NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
      cd12588
      Location:73143
      RRM1_p54nrb; RNA recognition motif 1 (RRM1) found in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)
      cl17169
      Location:149228
      RRM_SF; RNA recognition motif (RRM) superfamily

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000023.11 Reference GRCh38.p14 Primary Assembly

      Range
      71283635..71301168
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060947.1 Alternate T2T-CHM13v2.0

      Range
      69717744..69735278
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)