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    EPRS1 glutamyl-prolyl-tRNA synthetase 1 [ Homo sapiens (human) ]

    Gene ID: 2058, updated on 7-Apr-2024

    Summary

    Official Symbol
    EPRS1provided by HGNC
    Official Full Name
    glutamyl-prolyl-tRNA synthetase 1provided by HGNC
    Primary source
    HGNC:HGNC:3418
    See related
    Ensembl:ENSG00000136628 MIM:138295; AllianceGenome:HGNC:3418
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    EARS; EPRS; PARS; QARS; QPRS; HLD15; PIG32; GLUPRORS
    Summary
    Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in thyroid (RPKM 31.6), appendix (RPKM 22.6) and 25 other tissues See more
    Orthologs
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    Genomic context

    See EPRS1 in Genome Data Viewer
    Location:
    1q41
    Exon count:
    32
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (219968600..220046505, complement)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (219207867..219285874, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (220141942..220219847, complement)

    Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene solute carrier family 30 member 10 Neighboring gene Sharpr-MPRA regulatory region 9893 Neighboring gene uncharacterized LOC107985281 Neighboring gene small nucleolar RNA U3 Neighboring gene small nucleolar RNA U13 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_4879 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:220200073-220200574 Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:220219000-220219557 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2551 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2552 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_4904 Neighboring gene 3'(2'), 5'-bisphosphate nucleotidase 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2553 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2554 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1822 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1823 Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:220267703-220268202 Neighboring gene uncharacterized LOC105373475 Neighboring gene isoleucyl-tRNA synthetase 2, mitochondrial

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify glutamyl-prolyl-tRNA synthetase (EPRS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify glutamyl-prolyl-tRNA synthetase (EPRS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Nef nef Tandem affinity purification and mass spectrometry analysis identify glutamyl-prolyl-tRNA synthetase (EPRS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Pr55(Gag) gag Cellular biotinylated glutamyl-prolyl-tRNA synthetase (EPRS, Bifunctional glutamate/proline tRNA ligase) protein is incorporated into HIV-1 Gag virus-like particles PubMed
    gag Tandem affinity purification and mass spectrometry analysis identify glutamyl-prolyl-tRNA synthetase (EPRS), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    matrix gag HIV-1 MA is identified to have a physical interaction with glutamyl-prolyl-tRNA synthetase (EPRS) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • DKFZp313B047

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables GTPase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables RNA stem-loop binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables glutamate-tRNA ligase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables glutamate-tRNA ligase activity TAS
    Traceable Author Statement
    more info
     
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables proline-tRNA ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables proline-tRNA ligase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables proline-tRNA ligase activity TAS
    Traceable Author Statement
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein homodimerization activity IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Component Evidence Code Pubs
    part_of GAIT complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of aminoacyl-tRNA synthetase multienzyme complex IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    part_of aminoacyl-tRNA synthetase multienzyme complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in cytosol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in membrane HDA PubMed 
    located_in plasma membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    part_of ribonucleoprotein complex IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    bifunctional glutamate/proline--tRNA ligase
    Names
    bifunctional aminoacyl-tRNA synthetase
    cell proliferation-inducing gene 32 protein
    glutamate tRNA ligase
    glutamatyl-prolyl-tRNA synthetase
    glutaminyl-tRNA synthetase
    proliferation-inducing gene 32 protein
    proliferation-inducing protein 32
    proline-tRNA ligase
    prolyl-tRNA synthetase
    NP_004437.2

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_004446.3NP_004437.2  bifunctional glutamate/proline--tRNA ligase

      See identical proteins and their annotated locations for NP_004437.2

      Status: REVIEWED

      Source sequence(s)
      AA196283, AY493416, BC034797, BC046156, N85252
      Consensus CDS
      CCDS31027.1
      UniProtKB/Swiss-Prot
      A0AVA9, B9EGH3, P07814, Q05BP6, Q05DF8, Q5DSM1, Q5H9S5, Q6PD57, Q86X73
      Related
      ENSP00000355890.3, ENST00000366923.8
      Conserved Domains (9) summary
      cd10309
      Location:74157
      GST_C_GluProRS_N; Glutathione S-transferase C-terminal-like, alpha helical domain of bifunctional Glutamyl-Prolyl-tRNA synthetase
      PLN02907
      Location:13711
      PLN02907; glutamate-tRNA ligase
      PRK08661
      Location:10191512
      PRK08661; prolyl-tRNA synthetase; Provisional
      cd00778
      Location:10231286
      ProRS_core_arch_euk; Prolyl-tRNA synthetase (ProRS) class II core catalytic domain. ProRS is a homodimer. It is responsible for the attachment of proline to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent formation of ...
      cd00862
      Location:12921512
      ProRS_anticodon_zinc; ProRS Prolyl-anticodon binding domain, long version found predominantly in eukaryotes and archaea. ProRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in ...
      cd00936
      Location:904952
      WEPRS_RNA; WEPRS_RNA binding domain. This short RNA-binding domain is found in several higher eukaryote aminoacyl-tRNA synthetases (aaRSs). It is found in multiple copies in eukaryotic bifunctional glutamyl-prolyl-tRNA synthetases (EPRS) in a region that separates ...
      pfam00458
      Location:753805
      WHEP-TRS; WHEP-TRS domain
      pfam00749
      Location:197502
      tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain
      pfam03950
      Location:504681
      tRNA-synt_1c_C; tRNA synthetases class I (E and Q), anti-codon binding domain

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

      Range
      219968600..220046505 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060925.1 Alternate T2T-CHM13v2.0

      Range
      219207867..219285874 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)