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    St8sia4 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 [ Mus musculus (house mouse) ]

    Gene ID: 20452, updated on 21-Apr-2024

    Summary

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    Official Symbol
    St8sia4provided by MGI
    Official Full Name
    ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4provided by MGI
    Primary source
    MGI:MGI:106018
    See related
    Ensembl:ENSMUSG00000040710 AllianceGenome:MGI:106018
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    PST; PST-1; Siat8d; SIAT8-D; ST8SiaIV
    Summary
    Enables alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity. Predicted to be involved in ganglioside biosynthetic process; glycoprotein metabolic process; and oligosaccharide metabolic process. Predicted to be located in Golgi apparatus and membrane. Predicted to be integral component of membrane. Is expressed in several structures, including central nervous system; genitourinary system; gut; hemolymphoid system gland; and lung. Orthologous to human ST8SIA4 (ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in CNS E18 (RPKM 5.6), whole brain E14.5 (RPKM 4.9) and 19 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    1 D; 1 47.76 cM
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (95515407..95595473, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (95587682..95667717, complement)

    Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_01757 Neighboring gene predicted pseudogene 15427 Neighboring gene U7 small nuclear RNA, pseudogene 2 Neighboring gene STARR-positive B cell enhancer ABC_E987 Neighboring gene STARR-positive B cell enhancer ABC_E11079 Neighboring gene STARR-positive B cell enhancer ABC_E10023 Neighboring gene STARR-positive B cell enhancer mm9_chr1:97507476-97507777 Neighboring gene STARR-positive B cell enhancer mm9_chr1:97515913-97516213 Neighboring gene STARR-positive B cell enhancer ABC_E11080 Neighboring gene STARR-positive B cell enhancer ABC_E1225 Neighboring gene STARR-positive B cell enhancer ABC_E10024 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:97562784-97562967 Neighboring gene STARR-positive B cell enhancer ABC_E7736 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:97564187-97564374 Neighboring gene predicted gene, 30431 Neighboring gene STARR-positive B cell enhancer mm9_chr1:97589059-97589359 Neighboring gene RIKEN cDNA 4930598F16 gene Neighboring gene STARR-seq mESC enhancer starr_01758 Neighboring gene STARR-seq mESC enhancer starr_01759 Neighboring gene STARR-seq mESC enhancer starr_01760 Neighboring gene STARR-positive B cell enhancer ABC_E5786 Neighboring gene predicted gene, 22360

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Environmental enrichment rescues memory in mice deficient for the polysialytransferase ST8SiaIV. Zerwas M, et al. Brain Struct Funct, 2016 Apr. PMID 25596866
    2. Polysialyltransferase overexpression in Schwann cells mediates different effects during peripheral nerve regeneration. Jungnickel J, et al. Glycobiology, 2012 Jan. PMID 21840969
    3. Remyelination after cuprizone induced demyelination is accelerated in mice deficient in the polysialic acid synthesizing enzyme St8siaIV. Koutsoudaki PN, et al. Neuroscience, 2010 Nov 24. PMID 20833231
    4. Polysialic acid affects pathophysiological consequences of status epilepticus. Pekcec A, et al. Neuroreport, 2010 Jun 2. PMID 20351597
    5. Polysialic acid governs T-cell development by regulating progenitor access to the thymus. Drake PM, et al. Proc Natl Acad Sci U S A, 2009 Jul 21. PMID 19587240, Free PMC Article

    See all (63) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Mice deficient for ST8SiaIV have impaired long-term spatial memory in two tasks that critically rely on the hippocampus.
      Title: Environmental enrichment rescues memory in mice deficient for the polysialytransferase ST8SiaIV.
    2. Results demonstrate that the early development of calbindin-positive periglomerular interneurons depends on the presentation of polysialic acid on NCAM and requires the activity of both St8sia2 and St8sia4
      Title: Deficits of olfactory interneurons in polysialyltransferase- and NCAM-deficient mice.
    3. NCAM-140 significantly promoted cell proliferation, motility and migration, while polysialylation of NCAM-140 catalyzed by STX, but not by PST, enhanced NCAM-mediated cell migration, but not cell proliferation or motility.
      Title: Promotion of cell migration by neural cell adhesion molecule (NCAM) is enhanced by PSA in a polysialyltransferase-specific manner.
    4. ST8SiaIV synthesized polySia selectively on a NRP2 glycoform that was characterized by the presence of sialylated core 1 and core 2 O-glycans.
      Title: Polysialic acid on neuropilin-2 is exclusively synthesized by the polysialyltransferase ST8SiaIV and attached to mucin-type o-glycans located between the b2 and c domain.
    5. Results indicate that the polySTs ST8SiaII and ST8SiaIV not only recognize an acidic patch in the FN1 domain of NCAM but also must contact sequences in the Ig5 domain for polysialylation of Ig5 N-glycans to occur.
      Title: The polysialyltransferases interact with sequences in two domains of the neural cell adhesion molecule to allow its polysialylation.
    6. the negative and positive effects of ST8SiaIV overexpression observed during peripheral nerve regeneration suggest that an optimized time- and differentiation-dependent control of expression in Schwann cells may further improve nerve regeneration
      Title: Polysialyltransferase overexpression in Schwann cells mediates different effects during peripheral nerve regeneration.
    7. While both ST8SiaII and ST8SiaIV mRNA levels decrease around the time of eye opening in mouse visual cortex, only ST8SiaII mRNA level reduction is regulated by sensory experience
      Title: Sensory experience differentially modulates the mRNA expression of the polysialyltransferases ST8SiaII and ST8SiaIV in postnatal mouse visual cortex.
    8. ST8Sia4 knockout mice display a decreased motivation in social interaction. This deficit can be partly explained by olfactory deficits and was associated with a clear decrease in PSA-NCAM expression in all brain regions analyzed.
      Title: Differential impact of polysialyltransferase ST8SiaII and ST8SiaIV knockout on social interaction and aggression.
    9. This study demonistrated that mice lacking St8siaIV polysialyltransferase have an accelerated onset of remyelination after cuprizone-induced demyelination.
      Title: Remyelination after cuprizone induced demyelination is accelerated in mice deficient in the polysialic acid synthesizing enzyme St8siaIV.
    10. In summary, the data suggest that the polysialic acid-neural cell adhesion molecule system is a putative target for the modulation of
      Title: Polysialic acid affects pathophysiological consequences of status epilepticus.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (2)  1 citation
    • Endonuclease-mediated (2) 

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables sialyltransferase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in N-glycan processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in N-glycan processing ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in ganglioside biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in oligosaccharide metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in oligosaccharide metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein glycosylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein glycosylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in sialylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in sialylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi apparatus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase
    Names
    alpha-2,8-sialyltransferase 8D
    polysialyltransferase-1
    sialyltransferase 8 (alpha-2, 8-sialyltransferase) D
    sialyltransferase 8D
    sialyltransferase St8Sia IV
    sialytransferase St8Sia IV
    NP_001153217.1
    NP_001407932.1
    NP_001407933.1
    NP_001407934.1
    NP_033209.1
    XP_036018814.1
    XP_036018816.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001159745.2 → NP_001153217.1  CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase isoform 2 precursor

      Status: VALIDATED

      Source sequence(s)
      AC102483, AC104893
      Consensus CDS
      CCDS78661.1
      UniProtKB/TrEMBL
      A0A087WRA0
      Related
      ENSMUSP00000140534.2, ENSMUST00000189556.2
      Conserved Domains (1) summary
      pfam00777
      Location:94 → 262
      Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)

    2. NM_001421003.1 → NP_001407932.1  CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase isoform 3 precursor

      Status: VALIDATED

      Source sequence(s)
      AC102483, AC104893

    3. NM_001421004.1 → NP_001407933.1  CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase isoform 4 precursor

      Status: VALIDATED

      Source sequence(s)
      AC102483, AC104893

    4. NM_001421005.1 → NP_001407934.1  CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase isoform 5 precursor

      Status: VALIDATED

      Source sequence(s)
      AC102483, AC104893

    5. NM_009183.3 → NP_033209.1  CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase isoform 1 precursor

      See identical proteins and their annotated locations for NP_033209.1

      Status: VALIDATED

      Source sequence(s)
      AC102483, AC104893
      Consensus CDS
      CCDS15201.1
      UniProtKB/Swiss-Prot
      Q64692
      UniProtKB/TrEMBL
      Q53WR7, Q6PAS1, Q8BY70
      Related
      ENSMUSP00000043477.5, ENSMUST00000043336.11
      Conserved Domains (1) summary
      pfam00777
      Location:94 → 353
      Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)

    RNA

    1. NR_185227.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC102483, AC104893

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000067.7 Reference GRCm39 C57BL/6J

      Range
      95515407..95595473 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036162921.1 → XP_036018814.1  CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase isoform X1

      Conserved Domains (1) summary
      pfam00777
      Location:189 → 357
      Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)

    2. XM_036162923.1 → XP_036018816.1  CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase isoform X2

      Conserved Domains (1) summary
      pfam00777
      Location:189 → 357
      Glyco_transf_29; Glycosyltransferase family 29 (sialyltransferase)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q64692.1 GenPept UniProtKB/Swiss-Prot:Q64692

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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