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    CHEK1 checkpoint kinase 1 [ Homo sapiens (human) ]

    Gene ID: 1111, updated on 22-Jan-2023

    Summary

    Official Symbol
    CHEK1provided by HGNC
    Official Full Name
    checkpoint kinase 1provided by HGNC
    Primary source
    HGNC:HGNC:1925
    See related
    Ensembl:ENSG00000149554 MIM:603078; AllianceGenome:HGNC:1925
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    CHK1
    Summary
    The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]
    Expression
    Broad expression in bone marrow (RPKM 4.6), lymph node (RPKM 3.5) and 23 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See CHEK1 in Genome Data Viewer
    Location:
    11q24.2
    Exon count:
    17
    Annotation release Status Assembly Chr Location
    110 current GRCh38.p14 (GCF_000001405.40) 11 NC_000011.10 (125625136..125681124)
    110 current T2T-CHM13v2.0 (GCF_009914755.1) 11 NC_060935.1 (125653757..125704891)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (125495031..125546150)

    Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene STT3A antisense RNA 1 Neighboring gene RNA, U6 small nuclear 1156, pseudogene Neighboring gene EI24 autophagy associated transmembrane protein Neighboring gene STT3 oligosaccharyltransferase complex catalytic subunit A Neighboring gene uncharacterized LOC118567325 Neighboring gene RNA, U2 small nuclear 35, pseudogene Neighboring gene uncharacterized LOC105369551 Neighboring gene acrosomal vesicle protein 1 Neighboring gene prostate and testis expressed 1

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    Copy number response

    Description
    Copy number response
    Triplosensitivity

    No evidence available (Last evaluated 2021-11-10)

    ClinGen Genome Curation Page
    Haploinsufficency

    No evidence available (Last evaluated 2021-11-10)

    ClinGen Genome Curation PagePubMed

    HIV-1 interactions

    Protein interactions

    Protein Gene Interaction Pubs
    Vpr vpr HIV-1 Vpr activates ATR, resulting in phosphorylation of Chk1 and activation of the ATR-mediated DNA damage response PubMed
    vpr Phosphorylation of Chk1 at position Ser345 is required for HIV-1 Vpr-induced G2/M arrest, possibly through signaling of DNA re-replication via Cdt1 PubMed
    vpr ATR-induced phosphorylation of Chk1-Ser(345) requires PP2A only when Vpr activates ATR PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables histone H3T11 kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein domain specific binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    enables protein serine kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein serine/threonine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    enables protein serine/threonine kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables protein serine/threonine kinase activity TAS
    Traceable Author Statement
    more info
     
    Process Evidence Code Pubs
    involved_in DNA damage checkpoint signaling IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in DNA damage checkpoint signaling IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in DNA repair IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in DNA replication TAS
    Traceable Author Statement
    more info
     
    involved_in G2/M transition of mitotic cell cycle IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in apoptotic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in apoptotic process involved in development IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cellular response to DNA damage stimulus IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in cellular response to caffeine IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cellular response to mechanical stimulus IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    involved_in histone modification IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in inner cell mass cell proliferation IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in intracellular signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in mitotic G2 DNA damage checkpoint signaling IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in mitotic G2/M transition checkpoint IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in negative regulation of DNA biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in negative regulation of G0 to G1 transition TAS
    Traceable Author Statement
    more info
     
    involved_in negative regulation of mitotic nuclear division IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in nucleus organization IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in peptidyl-threonine phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of cell cycle IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in protein phosphorylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of cell population proliferation IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in regulation of double-strand break repair via homologous recombination IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of histone H3-K9 acetylation ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in regulation of mitotic centrosome separation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of signal transduction by p53 class mediator TAS
    Traceable Author Statement
    more info
     
    involved_in regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in replicative senescence NAS
    Non-traceable Author Statement
    more info
    PubMed 
    involved_in signal transduction in response to DNA damage IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in transcription initiation-coupled chromatin remodeling ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    Component Evidence Code Pubs
    located_in centrosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in chromatin ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    colocalizes_with chromosome, telomeric region IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in condensed nuclear chromosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in extracellular space HDA PubMed 
    located_in intracellular membrane-bounded organelle IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in replication fork IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    serine/threonine-protein kinase Chk1
    Names
    CHK1 checkpoint homolog
    Checkpoint, S. pombe, homolog of, 1
    Chk1-S
    cell cycle checkpoint kinase
    NP_001107593.1
    NP_001107594.1
    NP_001231775.1
    NP_001265.2
    NP_001317356.2
    NP_001317357.1
    XP_011540862.1
    XP_024304105.1
    XP_047282267.1
    XP_047282268.1
    XP_047282269.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_030049.3 RefSeqGene

      Range
      5029..37013
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001114121.2NP_001107593.1  serine/threonine-protein kinase Chk1 isoform 1

      See identical proteins and their annotated locations for NP_001107593.1

      Status: REVIEWED

      Source sequence(s)
      AK300082, AW149126, BC004202, BX419129, CA441277, CA868104, DA567616
      Consensus CDS
      CCDS8459.1
      UniProtKB/Swiss-Prot
      H2BI51, O14757
      UniProtKB/TrEMBL
      B4DT73
      Related
      ENSP00000412504.2, ENST00000428830.6
      Conserved Domains (1) summary
      cd14069
      Location:7265
      STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1
    2. NM_001114122.3NP_001107594.1  serine/threonine-protein kinase Chk1 isoform 1

      See identical proteins and their annotated locations for NP_001107594.1

      Status: REVIEWED

      Source sequence(s)
      AP001132
      Consensus CDS
      CCDS8459.1
      UniProtKB/Swiss-Prot
      H2BI51, O14757
      UniProtKB/TrEMBL
      B4DT73
      Related
      ENSP00000388648.1, ENST00000438015.7
      Conserved Domains (1) summary
      cd14069
      Location:7265
      STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1
    3. NM_001244846.1NP_001231775.1  serine/threonine-protein kinase Chk1 isoform 2

      Status: REVIEWED

      Source sequence(s)
      AA224205, AI750792, AK293143, AK300082, AW149126, BC004202, BM455102, BX114781, BX419129, BX506561, CA441277, CA868104, DA567616
      Consensus CDS
      CCDS58191.1
      UniProtKB/Swiss-Prot
      O14757
      UniProtKB/TrEMBL
      B4DT73
      Related
      ENSP00000278916.4, ENST00000278916.8
      Conserved Domains (2) summary
      smart00220
      Location:9264
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd14069
      Location:7265
      STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1
    4. NM_001274.5NP_001265.2  serine/threonine-protein kinase Chk1 isoform 1

      See identical proteins and their annotated locations for NP_001265.2

      Status: REVIEWED

      Source sequence(s)
      AA224205, AI750792, BC004202, BC017575, BG191840, BM455102, BP203333, BX114781, BX506561, CA441277
      Consensus CDS
      CCDS8459.1
      UniProtKB/Swiss-Prot
      H2BI51, O14757
      Related
      ENSP00000435371.1, ENST00000534070.5
      Conserved Domains (1) summary
      cd14069
      Location:7265
      STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1
    5. NM_001330427.2NP_001317356.2  serine/threonine-protein kinase Chk1 isoform 3

      Status: REVIEWED

      Source sequence(s)
      AP001132
      Conserved Domains (1) summary
      cl21453
      Location:1164
      PKc_like; Protein Kinases, catalytic domain
    6. NM_001330428.1NP_001317357.1  serine/threonine-protein kinase Chk1 isoform 4

      Status: REVIEWED

      Source sequence(s)
      AP001132
      Consensus CDS
      CCDS81645.1
      UniProtKB/Swiss-Prot
      O14757

    RNA

    1. NR_045204.1 RNA Sequence

      Status: REVIEWED

      Source sequence(s)
      AA224205, AI750792, AK300082, AW149126, BC004202, BG191840, BM455102, BX114781, BX419129, BX506561, CA441277, CA868104, DA567616
    2. NR_045205.1 RNA Sequence

      Status: REVIEWED

      Source sequence(s)
      AA224205, AI750792, AK292549, BC004202, BG191840, BG468734, BM455102, BX114781, BX506561, CA441277

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000011.10 Reference GRCh38.p14 Primary Assembly

      Range
      125625136..125681124
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011542560.3XP_011540862.1  serine/threonine-protein kinase Chk1 isoform X1

      See identical proteins and their annotated locations for XP_011540862.1

      UniProtKB/TrEMBL
      E7EPP6
      Related
      ENSP00000391090.2, ENST00000427383.6
      Conserved Domains (2) summary
      smart00220
      Location:117280
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cl21453
      Location:114281
      PKc_like; Protein Kinases, catalytic domain
    2. XM_024448337.2XP_024304105.1  serine/threonine-protein kinase Chk1 isoform X2

      UniProtKB/Swiss-Prot
      H2BI51
      Conserved Domains (1) summary
      cd14069
      Location:7265
      STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1
    3. XM_047426313.1XP_047282269.1  serine/threonine-protein kinase Chk1 isoform X4

      Related
      ENSP00000442317.2, ENST00000544373.5
    4. XM_047426311.1XP_047282267.1  serine/threonine-protein kinase Chk1 isoform X2

      UniProtKB/Swiss-Prot
      H2BI51
      Related
      ENSP00000432890.1, ENST00000524737.6
    5. XM_047426312.1XP_047282268.1  serine/threonine-protein kinase Chk1 isoform X3

    RNA

    1. XR_007062447.1 RNA Sequence

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060935.1 Alternate T2T-CHM13v2.0

      Range
      125653757..125704891
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)