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    PSME3 proteasome activator subunit 3 [ Homo sapiens (human) ]

    Gene ID: 10197, updated on 10-Dec-2024

    Summary

    Official Symbol
    PSME3provided by HGNC
    Official Full Name
    proteasome activator subunit 3provided by HGNC
    Primary source
    HGNC:HGNC:9570
    See related
    Ensembl:ENSG00000131467 MIM:605129; AllianceGenome:HGNC:9570
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
    Summary
    The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]
    Expression
    Ubiquitous expression in esophagus (RPKM 20.0), urinary bladder (RPKM 19.8) and 25 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See PSME3 in Genome Data Viewer
    Location:
    17q21.31
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 17 NC_000017.11 (42833397..42843760)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 17 NC_060941.1 (43690536..43700899)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (40985414..40995777)

    Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene cyclin N-terminal domain containing 1 Neighboring gene beclin 1 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr17:40969798-40970997 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 8548 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:40975527-40976093 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr17:40976094-40976661 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr17:40976662-40977228 Neighboring gene microRNA 6781 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 12227 Neighboring gene amine oxidase copper containing 2 Neighboring gene amine oxidase copper containing 3

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    Knockdown of proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki; PSME3) by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat HIV-1 Tat slightly enhances the activity of the purified 26 S proteasome PubMed
    tat Amino acids Lys51, Arg52, and Asp67 of HIV-1 Tat represent the proteasome binding site of Tat, and Tat amino acids 37-72 are necessary for proteasomal interaction and suppression of 11 S regulator-mediated antigen presentation PubMed
    tat HIV-1 Tat inhibits the peptidase activity of the 20 S proteasome and interferes with the formation of the 20 S proteasome-11 S regulator complex PubMed
    tat HIV-1 Tat binds to the alpha2, alpha4, alpha6, alpha7, beta1, beta2, beta3, beta5, beta6, beta7, LMP7/beta5i, and MECL1/beta2i subunits of the proteasome 20 S core structure and can inhibit cellular proteasome function PubMed
    Vif vif HIV-1 Vif is identified to have a physical interaction with proteasome activator subunit 3 (PSME3; PA28gamma) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
    vif HIV-1 Vif binds to the cellular cytidine deaminase APOBEC3G and targets it for degradation through an interaction with the proteasome, thereby inhibiting APOBEC3G mediated restriction of HIV-1 replication PubMed
    integrase gag-pol Proteasomal degradation of HIV-1 integrase in mammalian cells occurs by the N-end rule pathway PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables MDM2/MDM4 family protein binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables endopeptidase activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables endopeptidase activator activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables p53 binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
     
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in membrane HDA PubMed 
    is_active_in nucleoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    located_in nucleus HDA PubMed 
    part_of proteasome activator complex IEA
    Inferred from Electronic Annotation
    more info
     
    part_of proteasome complex TAS
    Traceable Author Statement
    more info
    PubMed 

    General protein information

    Preferred Names
    proteasome activator complex subunit 3
    Names
    11S regulator complex gamma subunit
    11S regulator complex subunit gamma
    Ki antigen
    Ki nuclear autoantigen
    REG gamma-3
    activator of multicatalytic protease subunit 3
    epididymis secretory protein Li 283
    proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
    proteasome activator 28 subunit gamma
    proteasome activator 28-gamma

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001267045.2NP_001253974.1  proteasome activator complex subunit 3 isoform 3

      See identical proteins and their annotated locations for NP_001253974.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is longer than isoform 1.
      Source sequence(s)
      AC016889, AI304773, BI464061, BX640801
      Consensus CDS
      CCDS59290.1
      UniProtKB/TrEMBL
      Q6FHK7
      Related
      ENSP00000409487.2, ENST00000441946.6
      Conserved Domains (2) summary
      pfam02251
      Location:2580
      PA28_alpha; Proteasome activator pa28 alpha subunit
      pfam02252
      Location:120262
      PA28_beta; Proteasome activator pa28 beta subunit
    2. NM_001330229.2NP_001317158.1  proteasome activator complex subunit 3 isoform 4

      Status: REVIEWED

      Source sequence(s)
      AC016889, CX866774
      Consensus CDS
      CCDS82133.1
      UniProtKB/TrEMBL
      B3KQ25
      Related
      ENSP00000441682.1, ENST00000545225.5
      Conserved Domains (1) summary
      pfam02252
      Location:48190
      PA28_beta; Proteasome activator pa28 beta subunit
    3. NM_005789.4NP_005780.2  proteasome activator complex subunit 3 isoform 1

      See identical proteins and their annotated locations for NP_005780.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes the predominant isoform (1).
      Source sequence(s)
      AC016889, AI304773, BC001423, BI464061
      Consensus CDS
      CCDS45689.1
      UniProtKB/Swiss-Prot
      A8K9A3, O35563, P61289, P97373, Q12920, Q13172, Q9BQD9
      UniProtKB/TrEMBL
      Q6FHK7, V9HWJ8
      Related
      ENSP00000466794.1, ENST00000590720.6
      Conserved Domains (2) summary
      pfam02251
      Location:969
      PA28_alpha; Proteasome activator pa28 alpha subunit
      pfam02252
      Location:109251
      PA28_beta; Proteasome activator pa28 beta subunit
    4. NM_176863.3NP_789839.1  proteasome activator complex subunit 3 isoform 2

      See identical proteins and their annotated locations for NP_789839.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2), uses ans alternate in-frame splice site, compared to variant 1. The resulting isoform (2) is longer than isoform 1.
      Source sequence(s)
      AC016889, AI304773, U11292
      Consensus CDS
      CCDS11442.1
      UniProtKB/TrEMBL
      Q6FHK7
      Related
      ENSP00000293362.1, ENST00000293362.7
      Conserved Domains (2) summary
      pfam02251
      Location:1268
      PA28_alpha; Proteasome activator pa28 alpha subunit
      pfam02252
      Location:108265
      PA28_beta; Proteasome activator pa28 beta subunit

    RNA

    1. NR_049772.2 RNA Sequence

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) lacks an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AC016889, AI304773, AK303211, BI464061

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000017.11 Reference GRCh38.p14 Primary Assembly

      Range
      42833397..42843760
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047435096.1XP_047291052.1  proteasome activator complex subunit 3 isoform X1

      UniProtKB/TrEMBL
      Q6FHK7

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060941.1 Alternate T2T-CHM13v2.0

      Range
      43690536..43700899
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054314668.1XP_054170643.1  proteasome activator complex subunit 3 isoform X1

      UniProtKB/TrEMBL
      Q6FHK7