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    WASF2 WASP family member 2 [ Homo sapiens (human) ]

    Gene ID: 10163, updated on 21-Mar-2023

    Summary

    Official Symbol
    WASF2provided by HGNC
    Official Full Name
    WASP family member 2provided by HGNC
    Primary source
    HGNC:HGNC:12733
    See related
    Ensembl:ENSG00000158195 MIM:605875; AllianceGenome:HGNC:12733
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    IMD2; SCAR2; WASF4; WAVE2; dJ393P12.2
    Summary
    This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
    Expression
    Ubiquitous expression in esophagus (RPKM 43.4), ovary (RPKM 33.8) and 25 other tissues See more
    Orthologs
    NEW
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    Try the new Transcript table

    Genomic context

    See WASF2 in Genome Data Viewer
    Location:
    1p36.11
    Exon count:
    9
    Annotation release Status Assembly Chr Location
    RS_2023_03 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (27404230..27490167, complement)
    RS_2023_03 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (27245554..27331475, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (27730734..27816678, complement)

    Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:27720717-27721514 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:27725651-27726603 Neighboring gene G protein-coupled receptor 3 Neighboring gene Sharpr-MPRA regulatory region 8314 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:27756559-27757532 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:27816920-27817464 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:27817465-27818009

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    HIV-1 infection (VSV-G pseudotyped) of CEMT4 T cells downregulates plasma membrane expression of WASF2 PubMed
    HIV-1 VLP production/release from Jurkat T lymphocytes requires an intact RAC1-WASF2-BAIAP2-ARP3 pathway as demonstrated by siRNA knockdown experiments PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env HIV-1 gp120-mediated phosphorylation of WAVE2 requires both G(alpha)i-dependent and -independent pathways, and is conserved both in X4 and R5 viral infection of resting CD4 T cells and primary macrophages PubMed
    env HIV-1 triggers WAVE2 phosphorylation at serine 351 through HIV-1 gp120 binding to the chemokine coreceptor CXCR4 or CCR5 during entry PubMed
    env Downregulation of the Wave2 signaling complex (Tiam-1, Abl, Rac, IRSp53, Wave2, and Arp3) with siRNA reduces HIV-1 Env-mediated cell-cell fusion and virus-cell fusion PubMed
    Nef nef HIV-1-mediated effects on podosomes and migration involve Nef-HCK interaction, and HCK-mediated phosphorylation of WASP at podosomes PubMed
    Pr55(Gag) gag HIV-1 Gag intracellular localization is modulated by WASF2 and BAIAP2 as demonstrated by double siRNA knockdowns of these cellular proteins (in Jurkat T cells) PubMed
    gag HIV-1 Gag is required by RAC1-WASF2-BAIAP2-ARP3 pathway for HIV VLP production/release from Jurkat T lymphocytes PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables Arp2/3 complex binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    enables SH3 domain binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables actin binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables cadherin binding HDA PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein kinase A binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein kinase A regulatory subunit binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in Rac protein signal transduction IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in actin cytoskeleton organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in actin filament-based movement IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in ameboidal-type cell migration IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in angiogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in endocytosis IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in lamellipodium assembly IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in lamellipodium morphogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in megakaryocyte development IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in negative regulation of stress fiber assembly IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of Arp2/3 complex-mediated actin nucleation IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    involved_in positive regulation of lamellipodium assembly IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in postsynaptic actin cytoskeleton organization IEA
    Inferred from Electronic Annotation
    more info
     
    Component Evidence Code Pubs
    part_of SCAR complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    part_of SCAR complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of SCAR complex IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    located_in actin cytoskeleton TAS
    Traceable Author Statement
    more info
    PubMed 
    located_in basolateral plasma membrane IEA
    Inferred from Electronic Annotation
    more info
     
    located_in cell-cell junction IEA
    Inferred from Electronic Annotation
    more info
     
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in early endosome IEA
    Inferred from Electronic Annotation
    more info
     
    located_in extracellular exosome HDA PubMed 
    is_active_in lamellipodium IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    located_in lamellipodium IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of protein-containing complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in ruffle IEA
    Inferred from Electronic Annotation
    more info
     
    located_in synapse IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    actin-binding protein WASF2
    Names
    WAS protein family member 2
    WASP family Verprolin-homologous protein 2
    WASP family protein member 2
    WASP family protein member 4
    protein WAVE-2
    putative Wiskott-Aldrich syndrome protein family member 4
    suppressor of cyclic-AMP receptor (WASP-family)
    verprolin homology domain-containing protein 2
    wiskott-Aldrich syndrome protein family member 2

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001201404.3NP_001188333.1  actin-binding protein WASF2 isoform 2

      See identical proteins and their annotated locations for NP_001188333.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an alternate exon compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.
      Source sequence(s)
      AA736923, AK303011, FO393419, HY001891
      Consensus CDS
      CCDS55582.1
      UniProtKB/Swiss-Prot
      Q9Y6W5
      Related
      ENSP00000439883.1, ENST00000536657.1
    2. NM_006990.5NP_008921.1  actin-binding protein WASF2 isoform 1

      See identical proteins and their annotated locations for NP_008921.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AA736923, BC040943, DC300388, FO393419
      Consensus CDS
      CCDS304.1
      UniProtKB/Swiss-Prot
      Q9UDY7, Q9Y6W5
      Related
      ENSP00000483313.1, ENST00000618852.5
      Conserved Domains (2) summary
      cd21762
      Location:450453
      WH2; actin-binding sequence [structural motif]
      cl41728
      Location:428479
      WH2; Wiskott-Aldrich Syndrome Homology (WASP) region 2 (WH2 motif), and similar proteins

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_03

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

      Range
      27404230..27490167 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060925.1 Alternate T2T-CHM13v2.0

      Range
      27245554..27331475 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)