GCN4 amino acid starvation-responsive transcription factor GCN4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GCN4
Official Full Name: amino acid starvation-responsive transcription factor GCN4
Primary source: SGD:S000000735
Locus tag: YEL009C
See related: AllianceGenome:SGD:S000000735; FungiDB:YEL009C; VEuPathDB:YEL009C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AAS101; AAS3; ARG9
Summary: Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II-specific DNA-binding transcription factor binding activity; and chromatin binding activity. Involved in several processes, including positive regulation of cellular response to amino acid starvation; protein localization to nuclear periphery; and regulation of transcription by RNA polymerase II. Located in nucleus. [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See GCN4 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (138918..139763, complement)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Differential stability of Gcn4p controls its cell-specific activity in differentiated yeast colonies.
Title: Differential stability of Gcn4p controls its cell-specific activity in differentiated yeast colonies.
Multiomics of GCN4-Dependent Replicative Lifespan Extension Models Reveals Gcn4 as a Regulator of Protein Turnover in Yeast.
Title: Multiomics of GCN4-Dependent Replicative Lifespan Extension Models Reveals Gcn4 as a Regulator of Protein Turnover in Yeast.
Gcn4 impacts metabolic fluxes to promote yeast chronological lifespan.
Title: Gcn4 impacts metabolic fluxes to promote yeast chronological lifespan.
Stochastic scanning events on the GCN4 mRNA 5' untranslated region generate cell-to-cell heterogeneity in the yeast nutritional stress response.
Title: Stochastic scanning events on the GCN4 mRNA 5' untranslated region generate cell-to-cell heterogeneity in the yeast nutritional stress response.
Origin of translational control by eIF2alpha phosphorylation: insights from genome-wide translational profiling studies in fission yeast.
Title: Origin of translational control by eIF2α phosphorylation: insights from genome-wide translational profiling studies in fission yeast.
Mediator subunit Med15 dictates the conserved ""fuzzy"" binding mechanism of yeast transcription activators Gal4 and Gcn4.
Title: Mediator subunit Med15 dictates the conserved "fuzzy" binding mechanism of yeast transcription activators Gal4 and Gcn4.
Genome-scale reconstruction of Gcn4/ATF4 networks driving a growth program.
Title: Genome-scale reconstruction of Gcn4/ATF4 networks driving a growth program.
ln4p depletion reduces this sequestration capacity, allowing uncharged tRNAGln to interact with Gcn2 kinase. The study sheds new light on mutant aaRS disease aetiologies, and explains how aaRS sequestration of uncharged tRNAs can prevent GCN4 activation under non-starvation conditions.
Title: The molecular aetiology of tRNA synthetase depletion: induction of a GCN4 amino acid starvation response despite homeostatic maintenance of charged tRNA levels.
eIF2beta or eIF1 substitutions disrupting these contacts increase initiation at UUG codons, and compound substitutions also derepress translation of GCN4, indicating slower TC recruitment.
Title: A network of eIF2β interactions with eIF1 and Met-tRNAi promotes accurate start codon selection by the translation preinitiation complex.
Authors find that the large Gcn4-Med15 complex is heterogeneous and contains nearly all possible AD-ABD interactions. Gcn4-Med15 forms via a dynamic fuzzy protein-protein interface, where ADs bind the ABDs in multiple orientations via hydrophobic regions that gain helicity.
Title: Gcn4-Mediator Specificity Is Mediated by a Large and Dynamic Fuzzy Protein-Protein Complex.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IPI Inferred from Physical Interaction more info	PubMed
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	HDA more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in amino acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in cellular response to nutrient levels	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of ribosomal protein gene transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nitrogen catabolite activation of transcription from RNA polymerase II promoter	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of RNA polymerase II transcription preinitiation complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of RNA polymerase II transcription preinitiation complex assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of RNA polymerase II transcription preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cellular response to amino acid starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cellular response to amino acid starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cellular response to amino acid starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to nuclear periphery	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: amino acid starvation-responsive transcription factor GCN4

NP_010907.3

bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels; contains four upstream open reading frames (uORFs) in 5' untranslated region which regulate translation; participates in sulfur starvation-induced autophagy along with Met4p, but is dispensable

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

138918..139763 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178824.3 → NP_010907.3 TPA: amino acid starvation-responsive transcription factor GCN4 [Saccharomyces cerevisiae S288C]

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLN9, P03068, P03069, Q70D88, Q70D91, Q70D96, Q70D99, Q70DA0, Q96UT3

UniProtKB/TrEMBL

A6ZQT9, B3LS06, N1P3V4

Conserved Domains (2) summary

cd12192
Location:106 → 134

GCN4_cent; GCN4 central activation domain-like acidic activation domain

cd12193
Location:226 → 278

bZIP_GCN4; Basic leucine zipper (bZIP) domain of General control protein GCN4: a DNA-binding and dimerization domain