CKAP5 cytoskeleton associated protein 5 [Homo sapiens (human)] - Gene

Summary

Official Symbol: CKAP5provided by HGNC
Official Full Name: cytoskeleton associated protein 5provided by HGNC
Primary source: HGNC:HGNC:28959
See related: Ensembl:ENSG00000175216 MIM:611142; AllianceGenome:HGNC:28959
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: TOG; MSPS; TOGp; CHTOG; ch-TOG
Summary: This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Expression: Ubiquitous expression in testis (RPKM 29.0), brain (RPKM 23.7) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 11p11.2

Exon count:: 44

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	11	NC_000011.10 (46743048..46846280, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	11	NC_060935.1 (46899874..47003159, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	11	NC_000011.9 (46764598..46867831, complement)

Chromosome 11 - NC_000011.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Competing endogenous RNA analysis reveals the regulatory potency of CKAP5 in HPV+ HNSCC.
Title: Competing endogenous RNA analysis reveals the regulatory potency of CKAP5 in HPV+ HNSCC.
TACC3-ch-TOG interaction regulates spindle microtubule assembly by controlling centrosomal recruitment of gamma-TuRC.
Title: TACC3-ch-TOG interaction regulates spindle microtubule assembly by controlling centrosomal recruitment of γ-TuRC.
Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity.
Title: Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity.
Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning and reduces neurodegeneration.
Title: Tyrosine phosphorylation regulates hnRNPA2 granule protein partitioning and reduces neurodegeneration.
chTOG is a conserved mitotic error correction factor.
Title: chTOG is a conserved mitotic error correction factor.
Eribulin binding to the tip of microtubules and subsequent loss of ch-TOG is a priming event leading to alterations in microtubule dynamics and breast cancer cell migration.
Title: Eribulin targets a ch-TOG-dependent directed migration of cancer cells.
Data show that cytoskeleton associated protein 5 (chTOG) only weakly promotes importin-regulated microtubule nucleation, but acts synergistically with microtubule- associated protein TPX2.
Title: Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module.
Aurora-A kinase does not regulate TACC3-chTOG complex formation, indicating that Aurora-A solely functions as a recruitment factor for the TACC3-chTOG complex to centrosomes and proximal mitotic spindles.
Title: The centrosomal adaptor TACC3 and the microtubule polymerase chTOG interact via defined C-terminal subdomains in an Aurora-A kinase-independent manner.
Data show that five genes CKAP5, KPNB1, RAN, TPX2 and KIF11 were shown to be essential for tumor cell survival in both head and neck squamous cell carcinoma (HNSCC)and non-small cell lung cancer (NSCLC), but most particularly in HNSCC.
Title: Functional genetic screens identify genes essential for tumor cell survival in head and neck and lung cancer.
These observations indicate that EB1 and ch-TOG regulate microtubule organisation differently via distinct regions in the plus ends of microtubules.
Title: Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Association of IFIH1 and other autoimmunity risk alleles with selective IgA deficiency. EBI GWAS Catalog EBI GWAS CatalogPubMed
New sequence variants associated with bone mineral density. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
integrase	gag-pol	Analysis of HIV-1 proviral integration sites in antiretroviral treatment patients indicates that CKAP5 gene favors HIV-1 integration for expansion and persistence of infected cells, suggesting HIV-1 IN interacts with CKAP5	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

STS-D43948 (e-PCR)
- UniSTS:69653

D11S1219 (e-PCR)
- UniSTS:62493

D11S4335 (e-PCR)
- UniSTS:62530

RH25324 (e-PCR)
- UniSTS:91820

D11S4375 (e-PCR)
- UniSTS:33464

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables cadherin binding	HDA more info	PubMed
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule plus end polymerase	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule plus-end binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ribonucleoprotein complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA transport	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within centrosome cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome duplication	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment or maintenance of microtubule cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule depolymerization	TAS Traceable Author Statement more info	PubMed
involved_in microtubule polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule polymerization	TAS Traceable Author Statement more info	PubMed
involved_in mitotic spindle organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of microtubule nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	HDA more info	PubMed
colocalizes_with microtubule cytoskeleton	ISS Inferred from Sequence or Structural Similarity more info	PubMed
colocalizes_with microtubule plus-end	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with microtubule plus-end	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info
located_in plasma membrane	IDA Inferred from Direct Assay more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle pole	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cytoskeleton-associated protein 5

Names: colonic and hepatic tumor over-expressed gene protein; colonic and hepatic tumor overexpressed gene protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.