| Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae. | Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae.
Brothers M, Rine J., Free PMC Article | 03/5/2022 |
| Sir3 mediates long-range chromosome interactions in budding yeast. | Sir3 mediates long-range chromosome interactions in budding yeast.
Ruault M, Scolari VF, Lazar-Stefanita L, Hocher A, Loïodice I, Koszul R, Taddei A., Free PMC Article | 02/12/2022 |
| Direct interaction of Sir3 with O-acetyl-ADP-ribose promotes the further spreading of Sir3 along telomeres. | The Capability of O-Acetyl-ADP-Ribose, an Epigenetic Metabolic Small Molecule, on Promoting the Further Spreading of Sir3 along the Telomeric Chromatin.
Tung SY, Wang SH, Lee SP, Tsai SP, Su KC, Shen HH, Hong JY, Tsai MS, Liou GG., Free PMC Article | 01/11/2020 |
| Yeast Sir2 and Sir3 heterochromatin proteins directly modify the local chromatin environment of euchromatic DNA replication origins. | Yeast heterochromatin regulators Sir2 and Sir3 act directly at euchromatic DNA replication origins.
Hoggard TA, Chang F, Perry KR, Subramanian S, Kenworthy J, Chueng J, Shor E, Hyland EM, Boeke JD, Weinreich M, Fox CA., Free PMC Article | 07/7/2018 |
| Here, the authors analyze binding of the Sir proteins to reconstituted mono-, di-, tri-, and tetra-nucleosomal chromatin templates and show that key Sir-Sir interactions bridge only sites on different nucleosomes but not sites on the same nucleosome, and are therefore 'interrupted' with respect to sites on the same nucleosome. | Heterochromatin assembly by interrupted Sir3 bridges across neighboring nucleosomes.
Behrouzi R, Lu C, Currie MA, Jih G, Iglesias N, Moazed D., Free PMC Article | 11/11/2017 |
| Repair of UV-induced DNA lesions in Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction. | Repair of UV-induced DNA lesions in natural Saccharomyces cerevisiae telomeres is moderated by Sir2 and Sir3, and inhibited by yKu-Sir4 interaction.
Guintini L, Tremblay M, Toussaint M, D'Amours A, Wellinger RE, Wellinger RJ, Conconi A., Free PMC Article | 09/9/2017 |
| silencing requires Sir2, a highly-conserved NAD(+)-dependent histone deacetylase. At locations other than the rDNA, silencing also requires additional Sir proteins, Sir1, Sir3, and Sir4 that together form a repressive heterochromatin-like structure termed silent chromatin. | The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.
Gartenberg MR, Smith JS., Free PMC Article | 05/27/2017 |
| results suggest that Sir3 exhibits novel binding parameters and gene regulatory functions at the CN binding domains. | Analysis of novel Sir3 binding regions in Saccharomyces cerevisiae.
Mitsumori R, Ohashi T, Kugou K, Ichino A, Taniguchi K, Ohta K, Uchida H, Oki M. | 02/4/2017 |
| Sir3 interacts with nucleosomal arrays with a stoichiometry of two Sir3 monomers per nucleosome. We also find that Sir3 fibres are less compact than canonical magnesium-induced 30 nm fibres. | Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibres.
Swygert SG, Manning BJ, Senapati S, Kaur P, Lindsay S, Demeler B, Peterson CL., Free PMC Article | 02/6/2016 |
| Direct interactions promote eviction of the Sir3 heterochromatin protein by the SWI/SNF chromatin remodeling enzyme. | Direct interactions promote eviction of the Sir3 heterochromatin protein by the SWI/SNF chromatin remodeling enzyme.
Manning BJ, Peterson CL., Free PMC Article | 05/2/2015 |
| Telomere clusters, their dynamics, and their nuclear distribution result from random motion, aggregation, and dissociation of telomeric regions, specifically determined by the amount of Sir3. | Spatial telomere organization and clustering in yeast Saccharomyces cerevisiae nucleus is generated by a random dynamics of aggregation-dissociation.
Hozé N, Ruault M, Amoruso C, Taddei A, Holcman D., Free PMC Article | 12/21/2013 |
| By X-ray crystallography, we show that the acetylated N terminus of Sir3 does not interact with the nucleosome directly. Instead, it stabilizes a nucleosome-binding loop in the BAH domain. | Nα-acetylated Sir3 stabilizes the conformation of a nucleosome-binding loop in the BAH domain.
Yang D, Fang Q, Wang M, Ren R, Wang H, He M, Sun Y, Yang N, Xu RM. | 11/30/2013 |
| The crystal structure of the N-terminally acetylated BAH domain of Sir3 bound to the nucleosome core particle reveals that the N-terminal acetylation stabilizes the interaction of Sir3 with the nucleosome. | The N-terminal acetylation of Sir3 stabilizes its binding to the nucleosome core particle.
Arnaudo N, Fernández IS, McLaughlin SH, Peak-Chew SY, Rhodes D, Martino F., Free PMC Article | 11/30/2013 |
| Sir3 binding generates a more stable nucleosome by clamping H4R17 and R19 to nucleosomal DNA, and raise the possibility that such induced changes in histone-DNA contacts play major roles in the regulation of chromatin structure. | Heterochromatin protein Sir3 induces contacts between the amino terminus of histone H4 and nucleosomal DNA.
Wang F, Li G, Altaf M, Lu C, Currie MA, Johnson A, Moazed D., Free PMC Article | 08/10/2013 |
| Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation. | Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation.
Oppikofer M, Kueng S, Keusch JJ, Hassler M, Ladurner AG, Gut H, Gasser SM., Free PMC Article | 04/6/2013 |
| examined gene expression and molecular markers of silencing at the silent mating type loci under conditions of limiting Sir3 protein | Sir3 and epigenetic inheritance of silent chromatin in Saccharomyces cerevisiae.
Motwani T, Poddar M, Holmes SG., Free PMC Article | 09/22/2012 |
| crystal structure of complex of Sir3 BAH domain and nucleosome core particle at 3.0 A resolution; structure explains how covalent modifications on H4K16 and H3K79 regulate formation of silencing complex containing the nucleosome as a central component | Structural basis of silencing: Sir3 BAH domain in complex with a nucleosome at 3.0 Å resolution.
Armache KJ, Garlick JD, Canzio D, Narlikar GJ, Kingston RE., Free PMC Article | 12/3/2011 |
| Sir3 promotes telomere clustering independently of silencing in yeast | Clustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast.
Ruault M, De Meyer A, Loïodice I, Taddei A., Free PMC Article | 06/25/2011 |
| Genome-wide nucleosome mapping revealed that Sir3 binding to subtelomeric regions was associated with overpackaging of subtelomeric promoters. | Dynamics of Sir3 spreading in budding yeast: secondary recruitment sites and euchromatic localization.
Radman-Livaja M, Ruben G, Weiner A, Friedman N, Kamakaka R, Rando OJ., Free PMC Article | 05/21/2011 |
| The functional specialization of Sir3,as a silencing protein was facilitated by the tandem duplication of the OIR domain in the Sir1 family, allowing distinct Sir1-Sir3 and Sir1-Orc1 interactions through OIR-BAH domain interactions. | Elaboration, diversification and regulation of the Sir1 family of silencing proteins in Saccharomyces.
Gallagher JE, Babiarz JE, Teytelman L, Wolfe KH, Rine J., Free PMC Article | 01/21/2010 |
| These results, together with the previously characterized interaction between the C-terminal region of Sir3 and the histone H3/H4 tails, suggest that Sir3 utilizes multiple domains to interact with nucleosomes. | Mutational analysis of the Sir3 BAH domain reveals multiple points of interaction with nucleosomes.
Sampath V, Yuan P, Wang IX, Prugar E, van Leeuwen F, Sternglanz R., Free PMC Article | 01/21/2010 |
| Role of nucleic acid binding in Sir3-dependent interactions with chromatin fibers is reported. | Role of nucleic acid binding in Sir3p-dependent interactions with chromatin fibers.
Adkins NL, McBryant SJ, Johnson CN, Leidy JM, Woodcock CL, Robert CH, Hansen JC, Georgel PT. | 01/21/2010 |
| Sir3p BAH domain directly binds the nucleosomal LRS domain. | Compensatory interactions between Sir3p and the nucleosomal LRS surface imply their direct interaction.
Norris A, Bianchet MA, Boeke JD., Free PMC Article | 01/21/2010 |
| Results underscore the importance of proper interactions between Sir3 and the nucleosome in silent chromatin assembly. | Sir3-nucleosome interactions in spreading of silent chromatin in Saccharomyces cerevisiae.
Buchberger JR, Onishi M, Li G, Seebacher J, Rudner AD, Gygi SP, Moazed D., Free PMC Article | 01/21/2010 |
| These results define how SIR3p may function as a chromatin architectural protein and provide new insight into the interplay between endogenous and protein-mediated chromatin fiber condensation pathways. | The silent information regulator 3 protein, SIR3p, binds to chromatin fibers and assembles a hypercondensed chromatin architecture in the presence of salt.
McBryant SJ, Krause C, Woodcock CL, Hansen JC., Free PMC Article | 01/21/2010 |