| Loop extrusion mediates physiological Igh locus contraction for RAG scanning. | Loop extrusion mediates physiological Igh locus contraction for RAG scanning.
Dai HQ, Hu H, Lou J, Ye AY, Ba Z, Zhang X, Zhang Y, Zhao L, Yoon HS, Chapdelaine-Williams AM, Kyritsis N, Chen H, Johnson K, Lin S, Conte A, Casellas R, Lee CS, Alt FW., Free PMC Article | 03/13/2021 |
| CtIP-mediated DNA resection is dispensable for IgH class switch recombination by alternative end-joining. | CtIP-mediated DNA resection is dispensable for IgH class switch recombination by alternative end-joining.
Wang XS, Zhao J, Wu-Baer F, Shao Z, Lee BJ, Cupo OM, Rabadan R, Gautier J, Baer R, Zha S., Free PMC Article | 12/26/2020 |
| Each of the two Igh alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination. | Independent recruitment of Igh alleles in V(D)J recombination.
Alves-Pereira CF, de Freitas R, Lopes T, Gardner R, Marta F, Vieira P, Barreto VM., Free PMC Article | 10/3/2015 |
| Data indicate that a regulatory element within the immunoglobulin heavy chain gene (3'IghRR) is a target of the aryl hydrocarbon receptor (AhR). | The aryl hydrocarbon receptor regulates an essential transcriptional element in the immunoglobulin heavy chain gene.
Wourms MJ, Sulentic CE., Free PMC Article | 07/25/2015 |
| Focused AgR chromatin analysis splits the Igh-SE into 2 active regions that belong to states 4 and 5. Only the hRE1 region functions as an enhancer in pro-B cells. It interacts with other Igh regulatory elements. hRE2 belongs to state 5. | Targeted chromatin profiling reveals novel enhancers in Ig H and Ig L chain Loci.
Predeus AV, Gopalakrishnan S, Huang Y, Tang J, Feeney AJ, Oltz EM, Artyomov MN., Free PMC Article | 03/29/2014 |
| Myc translocations are independent of chromosomal location and orientation of the immunoglobulin heavy chain locus | Oncogenic Myc translocations are independent of chromosomal location and orientation of the immunoglobulin heavy chain locus.
Spehalski E, Kovalchuk AL, Collins JT, Liang G, Dubois W, Morse HC 3rd, Ferguson DO, Casellas R, Dunnick WA., Free PMC Article | 11/3/2012 |
| Mapping of translocation breakpoints using an acetylated histone H3 lysine 9 chromatin immunoprecipitation sequencing approach reveals Igh fusions up to approximately 350 kb upstream of Myc or the related oncogene Mycn in Burkitt translocations. | Mouse model of endemic Burkitt translocations reveals the long-range boundaries of Ig-mediated oncogene deregulation.
Kovalchuk AL, Ansarah-Sobrinho C, Hakim O, Resch W, Tolarová H, Dubois W, Yamane A, Takizawa M, Klein I, Hager GL, Morse HC 3rd, Potter M, Nussenzweig MC, Casellas R., Free PMC Article | 09/22/2012 |
| findings show the 3' cis-regulatory region is transcribed and undergoes AID-mediated mutation and recombination around phylogenetically conserved switchlike DNA repeats | AID-driven deletion causes immunoglobulin heavy chain locus suicide recombination in B cells.
Péron S, Laffleur B, Denis-Lagache N, Cook-Moreau J, Tinguely A, Delpy L, Denizot Y, Pinaud E, Cogné M. | 06/2/2012 |
| We conclude that hs5-7, a high-density CTCF-binding region at the 3' end of the Igh locus, impacts usage of V(H) regions as far as 500 kb away. | Germline deletion of Igh 3' regulatory region elements hs 5, 6, 7 (hs5-7) affects B cell-specific regulation, rearrangement, and insulation of the Igh locus.
Volpi SA, Verma-Gaur J, Hassan R, Ju Z, Roa S, Chatterjee S, Werling U, Hou H Jr, Will B, Steidl U, Scharff M, Edelman W, Feeney AJ, Birshtein BK., Free PMC Article | 04/21/2012 |
| IgH 3' regulatory region mediated deregulation of c-myc can promote various types of B lymphoproliferation in cells that first acquired a p53 defect. | A p53 defect sensitizes various stages of B cell development to lymphomagenesis in mice carrying an IgH 3' regulatory region-driven c-myc transgene.
Fiancette R, Rouaud P, Vincent-Fabert C, Laffleur B, Magnone V, Cogné M, Denizot Y. | 01/14/2012 |
| These observations imply that the 3' RR has different loop interactions with target Igh sequences at different stages of B cell development and Igh regulation. | Interaction between the immunoglobulin heavy chain 3' regulatory region and the IgH transcription unit during B cell differentiation.
Ju Z, Chatterjee S, Birshtein BK., Free PMC Article | 12/24/2011 |
| Study shows that IgH locus conformation involves 2 levels of chromosomal compaction; 1st-locus folds into several multilooped domains, 2nd-these domains are brought into spatial proximity by Emu-dependent interactions within the V(H) region. | Two forms of loops generate the chromatin conformation of the immunoglobulin heavy-chain gene locus.
Guo C, Gerasimova T, Hao H, Ivanova I, Chakraborty T, Selimyan R, Oltz EM, Sen R., Free PMC Article | 12/10/2011 |
| Findings demonstrate that CTCF plays an important role in the 3D structure of the Igh locus. | CCCTC-binding factor (CTCF) and cohesin influence the genomic architecture of the Igh locus and antisense transcription in pro-B cells.
Degner SC, Verma-Gaur J, Wong TP, Bossen C, Iverson GM, Torkamani A, Vettermann C, Lin YC, Ju Z, Schulz D, Murre CS, Birshtein BK, Schork NJ, Schlissel MS, Riblet R, Murre C, Feeney AJ., Free PMC Article | 08/27/2011 |
| Data show that deletion of DNaseI hypersensitive sites does not have an obvious impact on the IgH locus or B cell development. | Analysis of mice lacking DNaseI hypersensitive sites at the 5' end of the IgH locus.
Perlot T, Pawlitzky I, Manis JP, Zarrin AA, Brodeur PH, Alt FW., Free PMC Article | 04/30/2011 |
| Serine 3 is a novel phospho-acceptor which, when mutated to alanine, leads to increased class switching and c-myc/IgH translocations without affecting AID levels or catalytic activity. | Amino-terminal phosphorylation of activation-induced cytidine deaminase suppresses c-myc/IgH translocation.
Gazumyan A, Timachova K, Yuen G, Siden E, Di Virgilio M, Woo EM, Chait BT, Reina San-Martin B, Nussenzweig MC, McBride KM., Free PMC Article | 02/26/2011 |
| We conclude that Emu contributes to, but is not essential for IgH transcription | Comparison of identical and functional Igh alleles reveals a nonessential role for Eμ in somatic hypermutation and class-switch recombination.
Li F, Yan Y, Pieretti J, Feldman DA, Eckhardt LA., Free PMC Article | 12/4/2010 |
| Data show that that deletion of the whole IgH 3' regulatory region (3'RR) allows normal maturation until the stage of IgM/IgD expressing lymphocytes, but nearly abrogates class switch recombination to all C(H) genes. | Genomic deletion of the whole IgH 3' regulatory region (hs3a, hs1,2, hs3b, and hs4) dramatically affects class switch recombination and Ig secretion to all isotypes.
Vincent-Fabert C, Fiancette R, Pinaud E, Truffinet V, Cogné N, Cogné M, Denizot Y. | 10/30/2010 |
| report on the development of a genetic system for the detection of T(12;15)(Igh-Myc) translocations in plasma cells of a mouse strain in which an enhanced green fluorescent protein (GFP)-encoding reporter gene has been targeted to Myc | Genetic reporter system for oncogenic Igh-Myc translocations in mice.
Takizawa M, Kim JS, Tessarollo L, McNeil N, Waldschmidt TJ, Casellas R, Ried T, Janz S., Free PMC Article | 08/16/2010 |
| our study highlights a major effect of the global ambiance of the IgH locus; enhancers demonstrated as being strongly synergistic in transgenes turn out to be redundant in their endogenous context | In vivo redundant function of the 3' IgH regulatory element HS3b in the mouse.
Bébin AG, Carrion C, Marquet M, Cogné N, Lecardeur S, Cogné M, Pinaud E. | 05/3/2010 |
| the Igh 3' regulatory region confers oncogenic activity by long-range and developmental stage-specific activation of translocated c-myc genes | Long-range oncogenic activation of Igh-c-myc translocations by the Igh 3' regulatory region.
Gostissa M, Yan CT, Bianco JM, Cogné M, Pinaud E, Alt FW., Free PMC Article | 02/1/2010 |
| Comparison of the distance distributions to computer simulations of alternative chromatin arrangements predicted that the Igh locus is organized into compartments containing clusters of loops separated by linkers. | The 3D structure of the immunoglobulin heavy-chain locus: implications for long-range genomic interactions.
Jhunjhunwala S, van Zelm MC, Peak MM, Cutchin S, Riblet R, van Dongen JJ, Grosveld FG, Knoch TA, Murre C., Free PMC Article | 01/21/2010 |
| Data show that Myc on Chromosome 15 is recruited to the same transcription factory as the Igh gene located on Chromosome 12; Myc and Igh are the most frequent translocation partners in plasmacytoma and Burkitt lymphoma. | Myc dynamically and preferentially relocates to a transcription factory occupied by Igh.
Osborne CS, Chakalova L, Mitchell JA, Horton A, Wood AL, Bolland DJ, Corcoran AE, Fraser P., Free PMC Article | 01/21/2010 |
| In the absence of C-NHEJ, this alternative end-joining pathway frequently joins Igh locus breaks to other chromosomes to generate translocations | IgH class switching and translocations use a robust non-classical end-joining pathway.
Yan CT, Boboila C, Souza EK, Franco S, Hickernell TR, Murphy M, Gumaste S, Geyer M, Zarrin AA, Manis JP, Rajewsky K, Alt FW. | 01/21/2010 |
| Pax5 expression in Pax5-/- pro-B cells induced large-scale contraction and distal VH-DJH rearrangements of the IgH locus | Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavy-chain gene.
Fuxa M, Skok J, Souabni A, Salvagiotto G, Roldan E, Busslinger M., Free PMC Article | 01/21/2010 |
| The combination of 3' and 5' IgH regulatory elements thus appears as a potentially useful cassette in transgenes that require a stringent and early B lineage-specific expression. | Combination of 3' and 5' IgH regulatory elements mimics the B-specific endogenous expression pattern of IgH genes from pro-B cells to mature B cells in a transgenic mouse model.
Guglielmi L, Le Bert M, Comte I, Dessain ML, Drouet M, Ayer-Le Lievre C, Cogné M, Denizot Y. | 01/21/2010 |