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    RFA3 Rfa3p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853266, updated on 13-Apr-2024

    Summary

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    Gene symbol
    RFA3
    Gene description
    Rfa3p
    Primary source
    SGD:S000003709
    Locus tag
    YJL173C
    See related
    AllianceGenome:SGD:S000003709
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    RPA14; RPA3
    Summary
    Enables double-stranded DNA binding activity and sequence-specific DNA binding activity. Involved in several processes, including DNA metabolic process; DNA unwinding involved in DNA replication; and protein ubiquitination. Located in chromosome, telomeric region and condensed nuclear chromosome. Part of DNA replication factor A complex. [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: X
    Exon count:
    1
    Sequence:
    Chromosome: X; NC_001142.9 (96161..96529, complement)

    Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene Swi3p Neighboring gene Kre9p Neighboring gene Gly-Xaa carboxypeptidase Neighboring gene Toh1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001142.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Essential functions of the 32 kDa subunit of yeast replication protein A. Dickson AM, et al. Nucleic Acids Res, 2009 Apr. PMID 19244309, Free PMC Article
    2. A structural and dynamic model for the assembly of Replication Protein A on single-stranded DNA. Yates LA, et al. Nat Commun, 2018 Dec 21. PMID 30575763, Free PMC Article
    3. Saccharomyces cerevisiae replication protein A binds to single-stranded DNA in multiple salt-dependent modes. Kumaran S, et al. Biochemistry, 2006 Oct 3. PMID 17002295, Free PMC Article
    4. Cloning of the large subunit of replication protein A (RPA) from yeast Saccharomyces cerevisiae and its DNA binding activity through redox potential. Jeong HS, et al. J Biochem Mol Biol, 2002 Mar 31. PMID 12297029
    5. Roles of replication protein-A subunits 2 and 3 in DNA replication fork movement in Saccharomyces cerevisiae. Maniar HS, et al. Genetics, 1997 Apr. PMID 9093844, Free PMC Article

    See all (98) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables double-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA topological change IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA unwinding involved in DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in double-strand break repair via homologous recombination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in establishment of protein localization IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in heteroduplex formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mitotic recombination IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in nucleotide-excision repair IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein ubiquitination IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in reciprocal meiotic recombination IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance via recombination IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance via telomerase IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in telomere maintenance via telomere lengthening IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of DNA replication factor A complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in chromosome, telomeric region IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    located_in condensed nuclear chromosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nuclear lumen IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Rfa3p
    NP_012362.1
    • Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001142.9 Reference assembly

      Range
      96161..96529 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181606.1 → NP_012362.1  TPA: Rfa3p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_012362.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VW15, P26755
      UniProtKB/TrEMBL
      A0A8H4FC32, A6ZQG0, G2WGK1
      Conserved Domains (1) summary
      pfam08661
      Location:1 → 122
      Rep_fac-A_3; Replication factor A protein 3
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    6I52_A.1
    P26755.1

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