Slc28a2 solute carrier family 28 member 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Slc28a2provided by RGD
Full Name: solute carrier family 28 member 2provided by RGD
Primary source: RGD:61840
See related: Ensembl:ENSRNOG00000028668 AllianceGenome:RGD:61840
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables nucleoside:sodium symporter activity. Involved in protein localization to cell surface and retina homeostasis. Located in coated vesicle; plasma membrane; and vesicle membrane. Orthologous to human SLC28A2 (solute carrier family 28 member 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Liver (RPKM 708.8), Heart (RPKM 216.1) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3q35

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (129778425..129841292)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (109324815..109387702)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (114355003..114647382)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The purpose of this study is to determine the localization of nucleoside transporters expressed within rat epididymis to demonstrate the potential of epididymal reabsorption. Using immunohistochemistry, we determined that equilibrative nucleoside transporter 1 (ENT1) is localized to the basolateral membrane of epithelial cells, ENT2 is expressed in the nucleus of the epithelium and CNT2 is expressed by basal cells.
Title: Localization of nucleoside transporters in rat epididymis.
rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane
Title: rCNT2 extracellular cysteines, Cys(615) and Cys(649), are important for maturation and sorting to the plasma membrane.
CNT2 is likely to modulate extracellular adenosine and cell energy balance in neuronal tissue.
Title: Hypoxia and P1 receptor activation regulate the high-affinity concentrative adenosine transporter CNT2 in differentiated neuronal PC12 cells.
Report induction of CNT2 in experimental diabetes mellitus.
Title: Mechanism of nucleoside uptake in rat placenta and induction of placental CNT2 in experimental diabetes.
the N-terminal tail of rCNT2 contains dual sorting signals. An acidic region is responsible for its proper stabilization at the plasma membrane, whereas the putative CK2 domain (Ser(46)) is implicated in the apical sorting of the transporter.
Title: Structural determinants for rCNT2 sorting to the plasma membrane of polarized and non-polarized cells.
Data concluded that CNT2 contributes considerably to nucleoside uptake in rat hepatocytes but not in mouse hepatocytes.
Title: Striking species difference in the contribution of concentrative nucleoside transporter 2 to nucleoside uptake between mouse and rat hepatocytes.
The relative distribution of SLC28 gene expression suggests a role for the proximal tubule in renal nucleoside clearance and an accessory role for CNT2 and CNT3, in adenosine-mediated regulation of collecting duct functions.
Title: Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes.
a new physiological role for the transporter in the modulation of extracellular adenosine levels and the sleep/wakefulness cycle
Title: Distribution of CNT2 and ENT1 transcripts in rat brain: selective decrease of CNT2 mRNA in the cerebral cortex of sleep-deprived rats.
Results identify the critical domains and amino acid residues that contribute to the observed difference in specificity between concentrative nucleoside transporter (CNT)2 orthologs.
Title: Molecular determinants of specificity for synthetic nucleoside analogs in the concentrative nucleoside transporter, CNT2.
Bile acids were found to increase CNT2-related transport activity in a K(ATP) channel-independent manner in both Fao hepatoma and rat liver parenchymal cells.
Title: Bile acids alter the subcellular localization of CNT2 (concentrative nucleoside cotransporter) and increase CNT2-related transport activity in liver parenchymal cells.

Submit: New GeneRIF Correction See all GeneRIFs (13)

General gene information

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Markers

Slc28a2 (e-PCR), detects polymorphism
- UniSTS:462698

RH131217 (e-PCR)
- UniSTS:214500

RH127610 (e-PCR)
- UniSTS:210920

Slc28a2 (e-PCR), detects polymorphism
- UniSTS:547067

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables neurotransmitter transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables nucleoside transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleoside:sodium symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables nucleoside:sodium symporter activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleoside:sodium symporter activity	ISO Inferred from Sequence Orthology more info
enables purine nucleobase transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables purine nucleoside transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables purine nucleoside transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables pyrimidine- and adenosine-specific:sodium symporter activity	ISO Inferred from Sequence Orthology more info
enables uridine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in adenosine transport	ISO Inferred from Sequence Orthology more info
involved_in inosine transport	ISO Inferred from Sequence Orthology more info
involved_in neurotransmitter transport	ISO Inferred from Sequence Orthology more info
involved_in nucleoside transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleoside transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in nucleoside transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in protein localization to cell surface	IDA Inferred from Direct Assay more info	PubMed
involved_in purine nucleobase transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in purine nucleoside transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in pyrimidine nucleobase transport	IEA Inferred from Electronic Annotation more info
involved_in pyrimidine-containing compound transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in retina homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in retina homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in uridine transmembrane transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in apicolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in apicolateral plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in brush border membrane	ISO Inferred from Sequence Orthology more info
located_in coated vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in vesicle membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: sodium/nucleoside cotransporter 2

Names: CNT 2; Na(+)/nucleoside cotransporter 2; SPNT; concentrative nucleoside transporter 2; rCNT2; sodium-coupled nucleoside transporter 2; sodium/nucleoside cotransporter 2-like; sodium/purine nucleoside cotransporter; solute carrier family 28 (concentrative nucleoside transporter), member 2; solute carrier family 28 (sodium-coupled nucleoside transporter), member 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031664.3 → NP_113852.2 sodium/nucleoside cotransporter 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/TrEMBL

F1LN73, Q91WW8

Related

ENSRNOP00000024658.7, ENSRNOT00000024658.8

Conserved Domains (1) summary

TIGR00804
Location:175 → 586

nupC; nucleoside transporter

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086021.1 Reference GRCr8

Range

129778425..129841292

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063284461.1 → XP_063140531.1 sodium/nucleoside cotransporter 2 isoform X1

UniProtKB/TrEMBL

F1LN73
XM_063284459.1 → XP_063140529.1 sodium/nucleoside cotransporter 2 isoform X1

UniProtKB/TrEMBL

F1LN73
XM_063284462.1 → XP_063140532.1 sodium/nucleoside cotransporter 2 isoform X1

UniProtKB/TrEMBL

F1LN73
XM_039105769.2 → XP_038961697.1 sodium/nucleoside cotransporter 2 isoform X1

UniProtKB/TrEMBL

F1LN73, Q91WW8

Conserved Domains (1) summary

TIGR00804
Location:175 → 586

nupC; nucleoside transporter
XM_063284460.1 → XP_063140530.1 sodium/nucleoside cotransporter 2 isoform X1

UniProtKB/TrEMBL

F1LN73
XM_039105768.2 → XP_038961696.1 sodium/nucleoside cotransporter 2 isoform X1

UniProtKB/TrEMBL

F1LN73, Q91WW8

Conserved Domains (1) summary

TIGR00804
Location:175 → 586

nupC; nucleoside transporter
XM_039105770.2 → XP_038961698.1 sodium/nucleoside cotransporter 2 isoform X1

UniProtKB/TrEMBL

F1LN73, Q91WW8

Conserved Domains (1) summary

TIGR00804
Location:175 → 586

nupC; nucleoside transporter