Stx1a syntaxin 1A (brain) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Stx1aprovided by MGI
Official Full Name: syntaxin 1A (brain)provided by MGI
Primary source: MGI:MGI:109355
See related: Ensembl:ENSMUSG00000007207 AllianceGenome:MGI:109355
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: HPC-1
Summary: Enables SNARE binding activity and calcium channel inhibitor activity. Involved in several processes, including modulation of chemical synaptic transmission; regulation of synaptic vesicle priming; and signal release. Acts upstream of or within calcium-ion regulated exocytosis. Located in several cellular components, including axon; nuclear membrane; and secretory vesicle. Is integral component of plasma membrane. Part of synaptobrevin 2-SNAP-25-syntaxin-1a complex. Is expressed in several structures, including adrenal gland; aorta; intercostal muscle; nervous system; and retina. Used to study disease of mental health. Human ortholog(s) of this gene implicated in Creutzfeldt-Jakob disease and Hirschsprung's disease. Orthologous to human STX1A (syntaxin 1A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in cortex adult (RPKM 99.5), frontal lobe adult (RPKM 66.0) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 G2; 5 74.95 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (135051473..135079954)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (135023482..135051100)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release rely on the integrity of the C-terminal half of the SNARE domain of syntaxin-1A.
Title: The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release rely on the integrity of the C-terminal half of the SNARE domain of syntaxin-1A.
Myosin Va, a Novel Interaction Partner of STXBP1, Is Required to Transport Syntaxin1A to the Plasma Membrane.
Title: Myosin Va, a Novel Interaction Partner of STXBP1, Is Required to Transport Syntaxin1A to the Plasma Membrane.
Syntaxin-1A modulates vesicle fusion in mammalian neurons via juxtamembrane domain dependent palmitoylation of its transmembrane domain.
Title: Syntaxin-1A modulates vesicle fusion in mammalian neurons via juxtamembrane domain dependent palmitoylation of its transmembrane domain.
Reexamination of N-terminal domains of syntaxin-1 in vesicle fusion from central murine synapses.
Title: Reexamination of N-terminal domains of syntaxin-1 in vesicle fusion from central murine synapses.
Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly.
Title: Munc13 binds and recruits SNAP25 to chaperone SNARE complex assembly.
Phosphorylation of Syntaxin-1a by casein kinase 2alpha regulates pre-synaptic vesicle exocytosis from the reserve pool.
Title: Phosphorylation of Syntaxin-1a by casein kinase 2α regulates pre-synaptic vesicle exocytosis from the reserve pool.
Microglial expression of GAT-1 in the cerebral cortex.
Title: Microglial expression of GAT-1 in the cerebral cortex.
The results demonstrate that MUNC18-1 regulates the F-actin network independently of syntaxin1 targeting via hydrophobicity in beta-sheet 10. The abnormally dense F-actin network in Munc18-1-deficient cells is not a rate-limiting barrier in secretory vesicle docking or fusion.
Title: MUNC18-1 regulates the submembrane F-actin network, independently of syntaxin1 targeting, via hydrophobicity in β-sheet 10.
The role of syntaxin 1A in GLP1 release from intestinal cells as a response to external stimuli is reported.
Title: The SNARE Protein Syntaxin-1a Plays an Essential Role in Biphasic Exocytosis of the Incretin Hormone Glucagon-Like Peptide 1.
proteins, such as syntaxin-1, Munc18-1, or SNAP-25, modulate alpha-synuclein neuropathy and/or are dysregulated in Alzheimer's disease, understanding this type of neurodegeneration may provide new links between synaptic defects and neurodegeneration in humans
Title: Early Golgi Abnormalities and Neurodegeneration upon Loss of Presynaptic Proteins Munc18-1, Syntaxin-1, or SNAP-25.

Submit: New GeneRIF Correction See all GeneRIFs (43)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (9) 1 citation
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Stx1a (e-PCR), detects polymorphism
- UniSTS:230798

MARC_14487-14488:1010605685:1 (e-PCR)
- UniSTS:267687

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables SNAP receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables SNARE binding	IBA Inferred from Biological aspect of Ancestor more info
enables SNARE binding	IDA Inferred from Direct Assay more info	PubMed
enables SNARE binding	ISO Inferred from Sequence Orthology more info	PubMed
enables calcium channel inhibitor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables calcium-dependent protein binding	ISO Inferred from Sequence Orthology more info
enables chloride channel inhibitor activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables myosin binding	ISO Inferred from Sequence Orthology more info
enables myosin head/neck binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables protein-macromolecule adaptor activity	ISO Inferred from Sequence Orthology more info
enables transmembrane transporter binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in SNARE complex assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within calcium-ion regulated exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in hormone secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in modulation of excitatory postsynaptic potential	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neurotransmitter transport	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of calcium ion-dependent exocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of catecholamine secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of excitatory postsynaptic potential	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of exocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neurotransmitter secretion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of neurotransmitter secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of norepinephrine secretion	ISO Inferred from Sequence Orthology more info
involved_in protein localization to membrane	ISO Inferred from Sequence Orthology more info
involved_in protein sumoylation	ISO Inferred from Sequence Orthology more info
involved_in regulated exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of exocytosis	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic vesicle priming	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to gravity	ISO Inferred from Sequence Orthology more info
involved_in secretion by cell	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle endocytosis	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic vesicle exocytosis	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle fusion to presynaptic active zone membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic vesicle fusion to presynaptic active zone membrane	ISO Inferred from Sequence Orthology more info
involved_in vesicle docking	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle docking	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of SNARE complex	IBA Inferred from Biological aspect of Ancestor more info
part_of SNARE complex	ISO Inferred from Sequence Orthology more info
is_active_in Schaffer collateral - CA1 synapse	ISO Inferred from Sequence Orthology more info
located_in acrosomal vesicle	EXP Inferred from Experiment more info	PubMed
located_in actomyosin	ISO Inferred from Sequence Orthology more info
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
is_active_in endomembrane system	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	ISO Inferred from Sequence Orthology more info	PubMed
located_in nuclear membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info	PubMed
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density membrane	ISO Inferred from Sequence Orthology more info
located_in presynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic active zone membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in presynaptic active zone membrane	ISO Inferred from Sequence Orthology more info
located_in presynaptic active zone membrane	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
located_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
located_in secretory granule	ISO Inferred from Sequence Orthology more info
located_in synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in synaptic vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in synaptic vesicle membrane	ISO Inferred from Sequence Orthology more info
located_in synaptic vesicle membrane	ISO Inferred from Sequence Orthology more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a complex	IDA Inferred from Direct Assay more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a complex	ISO Inferred from Sequence Orthology more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex	ISO Inferred from Sequence Orthology more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex	ISO Inferred from Sequence Orthology more info
part_of voltage-gated potassium channel complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: syntaxin-1A

Names: neuron-specific antigen HPC-1; syntaxin 1 a

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001359179.1 → NP_001346108.1 syntaxin-1A isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

Source sequence(s)

AC084109

UniProtKB/TrEMBL

Q5D0A4

Conserved Domains (2) summary

pfam00804
Location:30 → 202

Syntaxin

pfam05739
Location:203 → 254

SNARE; SNARE domain
NM_016801.4 → NP_058081.2 syntaxin-1A isoform 1

See identical proteins and their annotated locations for NP_058081.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

BC047133, BY240737

Consensus CDS

CCDS19731.1

UniProtKB/Swiss-Prot

O35526

UniProtKB/TrEMBL

Q497P1, Q5D0A4

Related

ENSMUSP00000005509.5, ENSMUST00000005509.11

Conserved Domains (3) summary

cd00179
Location:30 → 181

SynN; Syntaxin N-terminus domain; syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane; they are a family of receptors for intracellular transport vesicles; each target membrane may ...

cd15880
Location:191 → 259

SNARE_syntaxin1; SNARE motif of syntaxin 1

pfam00804
Location:30 → 227

Syntaxin; Syntaxin

RNA

NR_133683.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in an internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC084109

Related

ENSMUST00000150838.2
NR_152855.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses two alternate splice junctions in the 3' end compared to variant 1.

Source sequence(s)

AC084109

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

135051473..135079954

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006504400.5 → XP_006504463.1 syntaxin-1A isoform X1

UniProtKB/TrEMBL

D6RFB9

Conserved Domains (1) summary

pfam00804
Location:30 → 227

Syntaxin