Atg5 autophagy related 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Atg5provided by MGI
Official Full Name: autophagy related 5provided by MGI
Primary source: MGI:MGI:1277186
See related: Ensembl:ENSMUSG00000038160 AllianceGenome:MGI:1277186
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Apg5l; Atg5l; Paddy; 2010107M05Rik; 3110067M24Rik
Summary: The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Two transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Expression: Ubiquitous expression in placenta adult (RPKM 9.7), bladder adult (RPKM 9.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 B2; 10 23.24 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (44144325..44240295)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (44268322..44364299)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The role of ATG5 beyond Atg8ylation and autophagy.
Title: The role of ATG5 beyond Atg8ylation and autophagy.
ATG5 Knockdown Attenuates Ischemia-Reperfusion Injury by Reducing Excessive Autophagy-Induced Ferroptosis.
Title: ATG5 Knockdown Attenuates Ischemia‒Reperfusion Injury by Reducing Excessive Autophagy-Induced Ferroptosis.
TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
Title: TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
Autophagy protein 5 controls flow-dependent endothelial functions.
Title: Autophagy protein 5 controls flow-dependent endothelial functions.
ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.
Title: ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.
Loss of ATG5 in KRT14(+) cells leads to accumulated functional impairments of salivary glands via pyroptosis.
Title: Loss of ATG5 in KRT14(+) cells leads to accumulated functional impairments of salivary glands via pyroptosis.
MIEAP and ATG5 are tumor suppressors in a mouse model of BRAF(V600E)-positive thyroid cancer.
Title: MIEAP and ATG5 are tumor suppressors in a mouse model of BRAF(V600E)-positive thyroid cancer.
The core autophagy protein ATG5 controls the polarity of the Golgi apparatus and insulin secretion of pancreatic beta cells.
Title: The core autophagy protein ATG5 controls the polarity of the Golgi apparatus and insulin secretion of pancreatic beta cells.
ATG16L2 inhibits NLRP3 inflammasome activation through promoting ATG5-12-16L1 complex assembly and autophagy.
Title: ATG16L2 inhibits NLRP3 inflammasome activation through promoting ATG5-12-16L1 complex assembly and autophagy.
LncRNA CCAT1 Upregulates ATG5 to Enhance Autophagy and Promote Gastric Cancer Development by Absorbing miR-140-3p.
Title: LncRNA CCAT1 Upregulates ATG5 to Enhance Autophagy and Promote Gastric Cancer Development by Absorbing miR-140-3p.

Submit: New GeneRIF Correction See all GeneRIFs (129)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (3) 1 citation
Endonuclease-mediated (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW319544 (e-PCR)
- UniSTS:180036

Atg5 (e-PCR), detects polymorphism
- UniSTS:526933

C88337 (e-PCR)
- UniSTS:208512

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
contributes_to Atg8-family ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables Atg8-family ligase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in aggrephagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in aggrephagy	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within antigen processing and presentation of endogenous antigen	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within antigen processing and presentation of endogenous antigen	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within autophagosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagosome assembly	ISO Inferred from Sequence Orthology more info
involved_in autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in autophagy	ISO Inferred from Sequence Orthology more info
involved_in autophagy of mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
involved_in axonal transport	IDA Inferred from Direct Assay more info	PubMed
involved_in axonal transport	IEP Inferred from Expression Pattern more info	PubMed
involved_in axonal transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within blood vessel remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of cardiac muscle cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to nitrogen starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to nitrosative stress	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chaperone-mediated autophagy	ISO Inferred from Sequence Orthology more info
acts_upstream_of_positive_effect chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within defense response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in macroautophagy	ISO Inferred from Sequence Orthology more info
involved_in mitophagy	IMP Inferred from Mutant Phenotype more info
acts_upstream_of mucus secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of autophagic cell death	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of cardiac muscle cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of defense response to virus	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of defense response to virus	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of innate immune response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of phagocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info
involved_in negative regulation of type I interferon production	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative stranded viral RNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative thymic T cell selection	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative thymic T cell selection	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within otolith development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in piecemeal microautophagy of the nucleus	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of mucus secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of stress granule assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of viral translation	ISO Inferred from Sequence Orthology more info
involved_in post-translational protein modification	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein lipidation involved in autophagosome assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein lipidation involved in autophagosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of autophagosome maturation	ISO Inferred from Sequence Orthology more info
involved_in regulation of cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of cytokine production involved in immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of reactive oxygen species metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of release of sequestered calcium ion into cytosol	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to fungus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within vasodilation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ventricular cardiac muscle cell development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Atg12-Atg5-Atg16 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Atg12-Atg5-Atg16 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Atg12-Atg5-Atg16 complex	ISO Inferred from Sequence Orthology more info
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in autophagosome	IBA Inferred from Biological aspect of Ancestor more info
located_in autophagosome	IDA Inferred from Direct Assay more info	PubMed
located_in autophagosome	ISO Inferred from Sequence Orthology more info	PubMed
located_in axon	ISO Inferred from Sequence Orthology more info
located_in axoneme	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IEP Inferred from Expression Pattern more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info	PubMed
is_active_in mitochondria-associated endoplasmic reticulum membrane contact site	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondria-associated endoplasmic reticulum membrane contact site	IDA Inferred from Direct Assay more info	PubMed
is_active_in phagophore	IBA Inferred from Biological aspect of Ancestor more info
is_active_in phagophore assembly site membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in phagophore assembly site membrane	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	NAS Non-traceable Author Statement more info	PubMed
part_of transferase complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: autophagy protein 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001314013.2 → NP_001300942.1 autophagy protein 5 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate internal exon and initiates translation at a downstream start codon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.

Source sequence(s)

AC155642

UniProtKB/Swiss-Prot

Q99J83

Conserved Domains (1) summary

pfam04106
Location:2 → 166

APG5; Autophagy protein Apg5
NM_001358596.2 → NP_001345525.1 autophagy protein 5 isoform 3

Status: REVIEWED

Source sequence(s)

AC155642

Conserved Domains (1) summary

pfam04106
Location:79 → 160

APG5; Autophagy protein Apg5
NM_001428372.1 → NP_001415301.1 autophagy protein 5 isoform 1

Status: REVIEWED

Source sequence(s)

AC155642

UniProtKB/Swiss-Prot

Q99J83
NM_001428373.1 → NP_001415302.1 autophagy protein 5 isoform 4

Status: REVIEWED

Source sequence(s)

AC155642
NM_001428374.1 → NP_001415303.1 autophagy protein 5 isoform 5

Status: REVIEWED

Source sequence(s)

AC155642
NM_001428375.1 → NP_001415304.1 autophagy protein 5 isoform 6

Status: REVIEWED

Source sequence(s)

AC155642
NM_001428376.1 → NP_001415305.1 autophagy protein 5 isoform 7

Status: REVIEWED

Source sequence(s)

AC155642
NM_001428377.1 → NP_001415306.1 autophagy protein 5 isoform 8

Status: REVIEWED

Source sequence(s)

AC155642
NM_001428378.1 → NP_001415307.1 autophagy protein 5 isoform 9

Status: REVIEWED

Source sequence(s)

AC155642
NM_053069.7 → NP_444299.1 autophagy protein 5 isoform 1

See identical proteins and their annotated locations for NP_444299.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 4 both encode the same isoform (1).

Source sequence(s)

AC155642

Consensus CDS

CCDS23824.1

UniProtKB/Swiss-Prot

Q99J83

UniProtKB/TrEMBL

A0A385JBX3

Related

ENSMUSP00000044769.5, ENSMUST00000039286.5

Conserved Domains (1) summary

pfam04106
Location:79 → 270

APG5; Autophagy protein Apg5

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

44144325..44240295

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017313783.3 → XP_017169272.1 autophagy protein 5 isoform X2

Conserved Domains (1) summary

pfam04106
Location:79 → 189

APG5; Autophagy protein Apg5
XM_011243108.4 → XP_011241410.1 autophagy protein 5 isoform X1

Conserved Domains (1) summary

pfam04106
Location:79 → 270

APG5; Autophagy protein Apg5