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    CD63 CD63 molecule [ Homo sapiens (human) ]

    Gene ID: 967, updated on 5-Dec-2021

    Summary

    Official Symbol
    CD63provided by HGNC
    Official Full Name
    CD63 moleculeprovided by HGNC
    Primary source
    HGNC:HGNC:1692
    See related
    Ensembl:ENSG00000135404 MIM:155740
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    MLA1; ME491; LAMP-3; OMA81H; TSPAN30
    Summary
    The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
    Expression
    Ubiquitous expression in fat (RPKM 184.8), colon (RPKM 181.1) and 25 other tissues See more
    Orthologs
    NEW
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    Try the new Transcript table

    Genomic context

    See CD63 in Genome Data Viewer
    Location:
    12q13.2
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    109.20211119 current GRCh38.p13 (GCF_000001405.39) 12 NC_000012.12 (55725323..55729673, complement)
    105.20201022 previous assembly GRCh37.p13 (GCF_000001405.25) 12 NC_000012.11 (56119107..56123457, complement)

    Chromosome 12 - NC_000012.12Genomic Context describing neighboring genes Neighboring gene BLOC1S1-RDH5 readthrough Neighboring gene biogenesis of lysosomal organelles complex 1 subunit 1 Neighboring gene retinol dehydrogenase 5 Neighboring gene CD63 antisense RNA 1 Neighboring gene growth differentiation factor 11 Neighboring gene SAP domain containing ribonucleoprotein Neighboring gene ORMDL sphingolipid biosynthesis regulator 2 Neighboring gene DnaJ heat shock protein family (Hsp40) member C14

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    HIV-1 LAI replication requires CD63 expression as knockdown with shRNA decreases viral production in HeLa cells PubMed
    siRNA knockdown of CD63 in U1/HIV-1 cells (chronically infected monocytoid cells harboring 2 integrated copies of HIV provirus per cell) decreases CA (p24) levels in supernatants but does not affect intracellular CA (p24) levels PubMed
    siRNA knockdown of CD63 decreases CCR5-tropic (ADA) and dual-tropic (89.6) pseudotyped virus (luciferase reporter HIV backbone presumed) infection of MDMs, yet does not affect MLV- and VSV-pseudotyped virus infection, as measured by luciferase activity PubMed
    siRNA knockdown of CD63 decreases HIV production from peripheral blood lymphocytes and dendritic cells as measured by supernatant CA (p24) levels PubMed
    siRNA knockdown of CD63 decreases HIV production from monocyte-derived macrophages (MDMs) infected with HIV-1 (SX strain) at a low multiplicity of infection (MOI= 0.02) PubMed
    Knockdown of CD63 by siRNA inhibits HIV-1 infection and replication in human CD4+ T cells, macrophages, and peripheral blood lymphocytes PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env CD63 enhances HIV-1 gp120/gp41-mediated entry in human macrophages PubMed
    env HIV-1 Gag, CA, and gp120 proteins co-localizes with CD63 and acetylcholinesterase (ACHE)-associated exosomes from supernatants of HIV-1 expressing cells. The 5' end of the Gag p17 open reading frame is sufficient for HIV-1 RNA exosome incorporation PubMed
    env Recruitment of Env to tetraspanin-enriched micro domains (TEMs) is required for cell-cell fusion repression by CD9 and CD63 PubMed
    env Tetraspanin proteins, such as CD9, CD63, CD81, CD82, and CD231, are incorporated on the membrane of released virions in an HIV-1 envelope protein (Env)-independent manner and have the potential to inhibit HIV-1 Env-mediated infection PubMed
    Envelope surface glycoprotein gp160, precursor env HIV-1 Env co-localizes with CD63 in HIV-1 infected CD4+ T cells PubMed
    env Recruitment of Env to tetraspanin-enriched micro domains (TEMs) is required for cell-cell fusion repression by CD9 and CD63 PubMed
    env DLG1 knockdown is associated with the redistribution and colocalization of Env toward CD63 and CD82 positive vesicle-like structures PubMed
    env In the absence of Vpu, Env accumulates extensively within clathrin-coated endosomal structures, including the viral proteins Gag and MA; the tetraspanins CD63 and CD81; the adaptor protein complex AP-3; and AIP1/ALIX, a cellular cofactor for viral budding PubMed
    env CD63 localizes to endosome compartments and is incorporated into the viral envelope of macrophage-derived HIV-1 particles PubMed
    Envelope transmembrane glycoprotein gp41 env HIV-1 gp41 co-localizes predominantly with CD63 at the virological synapse of Jurkat cells PubMed
    env CD63 enhances HIV-1 gp120/gp41-mediated entry in human macrophages PubMed
    env Recruitment of Env to tetraspanin-enriched microdomains (TEMs) is required for cell-cell fusion repression by CD9 and CD63 PubMed
    env Tetraspanin proteins, such as CD9, CD63, CD81, CD82, and CD231, are incorporated on the membrane of released virions in an HIV-1 envelope protein (Env)-independent manner and have the potential to inhibit HIV-1 Env-mediated infection PubMed
    Nef nef HIV-1 Nef induces release of CD63 (MAL-dependent exosome marker) from Jurkat T cells transfected with DNA constructs coding for Nef-GFP PubMed
    nef Both HIV-1 Nef and Vpu downregulate the cell surface expression of CD63 (TSPAN30) PubMed
    nef HIV-1 Nef targets CD4 to CD63-containing lysosomes for Nef-induced degradation of CD4, which requires the VPS4-mediated ESCRT machinery PubMed
    nef HIV-1 Nef increases the production of exosomes, which form in the late endosomes and co-localizes with the late endosomal marker CD63 in SupT cells PubMed
    nef HIV-1 Nef increases the production of exosomes, co-localizes with exosomal proteins CD63, AIP/Alix, AChE, Hsc70, LAMP2, and annexin A2 in HeLa cells PubMed
    Pr55(Gag) gag Co-localization of HIV-1 Gag virus-like particles in THP-1/CD169YF cells is reduced within CD81+ compartments, but enhanced within CD63+ or LAMP1+ compartments PubMed
    gag HIV-1 Gag proteins co-localize with CD63 and CD81 in intracellular exosomes PubMed
    gag HIV-1 Gag, CA, and gp120 proteins co-localizes with CD63 and acetylcholinesterase (ACHE)-associated exosomes from supernatants of HIV-1 expressing cells. The 5' end of the Gag p17 open reading frame is sufficient for HIV-1 RNA exosome incorporation PubMed
    gag Tetherin co-localizes with HIV-1 Gag in CD81- and CD63-enriched intracellular virus-containing compartments in macrophages, and that a separate population of tetherin is located in the TGN PubMed
    gag The budding defect of p6-deficient HIV-1 Gag is caused primarily by C-terminal exposure of p1. The p1 domain of p6-deficient Gag acts as an inhibitory budding signal by blocking the interaction of Gag with CD63 and VPS4B PubMed
    gag Env-mediated cell-cell fusion repression by CD9 and CD63 requires the presence of HIV-1 Gag PubMed
    gag DLG1 knockdown is associated with the redistribution and colocalization of Gag toward CD63 and CD82 positive vesicle-like structures PubMed
    gag HIV-1 Gag proteins co-localize with tetraspanins CD9, CD81, and CD82 in CD63-enriched micro domains PubMed
    gag A dileucine-like motif (residues 321-322) in HIV-1 Gag regulates the assembly of Gag into CD63-positive multivesicular bodies PubMed
    gag In human macrophages, HIV-1 Gag proteins co-localize with MHC II (HLA-DR), CD63, and Lamp1 in MHC II compartments PubMed
    Tat tat siRNA-mediated CD63 down regulation reduces production of the early HIV protein Tat in both macrophages and a CD4(+) cell line PubMed
    Vpu vpu Both HIV-1 Nef and Vpu downregulate the cell surface expression of CD63 (TSPAN30) PubMed
    capsid gag HIV-1 Gag colocalizes with CD63 in U1/HIV-1 cells (chronically infected monocytoid cells harboring 2 integrated copies of HIV provirus per cell) PubMed
    gag siRNA knockdown of CD63 in U1/HIV-1 cells (chronically infected monocytoid cells harboring 2 integrated copies of HIV provirus per cell) affects CA (p24) levels in supernatants (decreases) but not intracellular CA (p24) levels PubMed
    gag HIV-1 Gag, CA, and gp120 proteins co-localizes with CD63 and acetylcholinesterase (ACHE)-associated exosomes from supernatants of HIV-1 expressing cells. The 5' end of the Gag p17 open reading frame is sufficient for HIV-1 RNA exosome incorporation PubMed
    gag CD63 silencing inhibits production of the late protein CA p24 in early HIV-1 replication events in both macrophages and a CD4(+) cell line PubMed
    gag In human macrophages, HIV-1 Capsid (p24) co-localizes with MHC II (HLA-DR), CD63, and Lamp1 in MHC II compartments PubMed
    matrix gag HIV-1 Gag colocalizes with CD63 in U1/HIV-1 cells (chronically infected monocytoid cells harboring 2 integrated copies of HIV provirus per cell) PubMed
    gag In the absence of Vpu, Env accumulates extensively within clathrin-coated endosomal structures, including the viral proteins Gag and MA; the tetraspanins CD63 and CD81; the adaptor protein complex AP-3; and AIP1/ALIX, a cellular cofactor for viral budding PubMed

    Go to the HIV-1, Human Interaction Database

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in azurophil granule membrane TAS
    Traceable Author Statement
    more info
     
    located_in cell surface IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in endosome lumen IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in endosome membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in extracellular exosome HDA PubMed 
    located_in extracellular exosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in extracellular space IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in integral component of plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    located_in intracellular membrane-bounded organelle IDA
    Inferred from Direct Assay
    more info
     
    located_in intrinsic component of plasma membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in late endosome membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in lysosomal membrane HDA PubMed 
    located_in lysosomal membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in melanosome IEA
    Inferred from Electronic Annotation
    more info
     
    located_in multivesicular body membrane HDA PubMed 
    located_in multivesicular body membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in multivesicular body, internal vesicle IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
     
    located_in platelet dense granule membrane TAS
    Traceable Author Statement
    more info
     

    General protein information

    Preferred Names
    CD63 antigen
    Names
    CD63 antigen (melanoma 1 antigen)
    granulophysin
    limp1
    lysosomal-associated membrane protein 3
    lysosome integral membrane protein 1
    lysosome-associated membrane glycoprotein 3
    melanoma-associated antigen ME491
    melanoma-associated antigen MLA1
    ocular melanoma-associated antigen
    tetraspanin-30
    tspan-30

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_008347.1 RefSeqGene

      Range
      4989..8684
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001257389.2NP_001244318.1  CD63 antigen isoform A

      See identical proteins and their annotated locations for NP_001244318.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) has an alternate 5' UTR exon, compared to variant 1. Variants 1, 3, 4, 5 and 10 encode the same isoform A.
      Source sequence(s)
      AC009779, BU960599, CB128206, CD250685, CN296813
      Consensus CDS
      CCDS8890.1
      UniProtKB/Swiss-Prot
      P08962
      UniProtKB/TrEMBL
      A0A024RB05
      Related
      ENSP00000447730.1, ENST00000549117.5
      Conserved Domains (2) summary
      cd03166
      Location:105203
      CD63_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), CD63 family. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment model spans ...
      pfam00335
      Location:10227
      Tetraspannin; Tetraspanin family
    2. NM_001257390.2NP_001244319.1  CD63 antigen isoform A

      See identical proteins and their annotated locations for NP_001244319.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) has an alternate 5' UTR exon, compared to variant 1. Variants 1, 3, 4, 5 and 10 encode the same isoform A.
      Source sequence(s)
      AC009779, BG478842, BU960599
      Consensus CDS
      CCDS8890.1
      UniProtKB/Swiss-Prot
      P08962
      UniProtKB/TrEMBL
      A0A024RB05
      Related
      ENSP00000393502.3, ENST00000420846.7
      Conserved Domains (2) summary
      cd03166
      Location:105203
      CD63_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), CD63 family. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment model spans ...
      pfam00335
      Location:10227
      Tetraspannin; Tetraspanin family
    3. NM_001257391.2NP_001244320.1  CD63 antigen isoform A

      See identical proteins and their annotated locations for NP_001244320.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (5) has an alternate 5' UTR segment, compared to variant 1. Variants 1, 3, 4, 5 and 10 encode the same isoform A.
      Source sequence(s)
      AC009779, AL582859, BU960599
      Consensus CDS
      CCDS8890.1
      UniProtKB/Swiss-Prot
      P08962
      UniProtKB/TrEMBL
      A0A024RB05
      Related
      ENSP00000449337.1, ENST00000552692.5
      Conserved Domains (2) summary
      cd03166
      Location:105203
      CD63_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), CD63 family. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment model spans ...
      pfam00335
      Location:10227
      Tetraspannin; Tetraspanin family
    4. NM_001257392.1NP_001244321.1  CD63 antigen isoform C

      See identical proteins and their annotated locations for NP_001244321.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (7) has an alternate splice site in the 5' coding region, compared to variant 1. The resulting isoform (C) lacks an internal segment, compared to isoform A.
      Source sequence(s)
      AF508304, BU960599, CN484590
      Consensus CDS
      CCDS58243.1
      UniProtKB/Swiss-Prot
      P08962
      Related
      ENSP00000446807.1, ENST00000552754.5
      Conserved Domains (2) summary
      cd03166
      Location:82180
      CD63_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), CD63 family. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment model spans ...
      pfam00335
      Location:28208
      Tetraspannin; Tetraspanin family
    5. NM_001257400.2NP_001244329.1  CD63 antigen isoform D precursor

      See identical proteins and their annotated locations for NP_001244329.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (8) contains a distinct 5' UTR exon and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (D) has a shorter N-terminus, compared to isoform A. Variants 8 and 9 encode the same isoform D.
      Source sequence(s)
      AC009779, BC013017, BG766593, BU960599
      Consensus CDS
      CCDS58242.1
      UniProtKB/Swiss-Prot
      P08962
      Related
      ENSP00000447356.1, ENST00000546939.5
      Conserved Domains (1) summary
      cd03166
      Location:23121
      CD63_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), CD63 family. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment model spans ...
    6. NM_001257401.2NP_001244330.1  CD63 antigen isoform D precursor

      See identical proteins and their annotated locations for NP_001244330.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (9) contains a distinct 5' UTR exon and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (D) has a shorter N-terminus, compared to isoform A. Variants 8 and 9 encode the same isoform D.
      Source sequence(s)
      AC009779, BQ434569, BU190521, BU960599
      Consensus CDS
      CCDS58242.1
      UniProtKB/Swiss-Prot
      P08962
      Related
      ENSP00000448091.1, ENST00000550776.5
      Conserved Domains (1) summary
      cd03166
      Location:23121
      CD63_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), CD63 family. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment model spans ...
    7. NM_001267698.2NP_001254627.1  CD63 antigen isoform A

      See identical proteins and their annotated locations for NP_001254627.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (10) has an alternate 5' UTR exon, compared to variant 1. Variants 1, 3, 4, 5 and 10 encode the same isoform A.
      Source sequence(s)
      AA583189, AC009779, BC002349, BU167765
      Consensus CDS
      CCDS8890.1
      UniProtKB/Swiss-Prot
      P08962
      UniProtKB/TrEMBL
      A0A024RB05
      Related
      ENSP00000449281.1, ENST00000552164.5
      Conserved Domains (2) summary
      cd03166
      Location:105203
      CD63_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), CD63 family. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment model spans ...
      pfam00335
      Location:10227
      Tetraspannin; Tetraspanin family
    8. NM_001780.6NP_001771.1  CD63 antigen isoform A

      See identical proteins and their annotated locations for NP_001771.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (A). Variants 1, 3, 4, 5 and 10 encode the same isoform A.
      Source sequence(s)
      AC009779, BU960599, EB386351
      Consensus CDS
      CCDS8890.1
      UniProtKB/Swiss-Prot
      P08962
      UniProtKB/TrEMBL
      A0A024RB05
      Related
      ENSP00000257857.4, ENST00000257857.9
      Conserved Domains (2) summary
      cd03166
      Location:105203
      CD63_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), CD63 family. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment model spans ...
      pfam00335
      Location:10227
      Tetraspannin; Tetraspanin family

    RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20211119

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p13 Primary Assembly

    Genomic

    1. NC_000012.12 Reference GRCh38.p13 Primary Assembly

      Range
      55725323..55729673 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_024449283.1XP_024305051.1  CD63 antigen isoform X1

      Conserved Domains (2) summary
      cd03166
      Location:105203
      CD63_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), CD63 family. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment model spans ...
      pfam00335
      Location:10227
      Tetraspannin; Tetraspanin family

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001040034.1: Suppressed sequence

      Description
      NM_001040034.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
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