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    PPP1R8 protein phosphatase 1 regulatory subunit 8 [ Homo sapiens (human) ]

    Gene ID: 5511, updated on 15-Apr-2019

    Summary

    Official Symbol
    PPP1R8provided by HGNC
    Official Full Name
    protein phosphatase 1 regulatory subunit 8provided by HGNC
    Primary source
    HGNC:HGNC:9296
    See related
    Ensembl:ENSG00000117751 MIM:602636
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
    Summary
    This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in endometrium (RPKM 13.4), lymph node (RPKM 13.2) and 25 other tissues See more
    Orthologs

    Genomic context

    See PPP1R8 in Genome Data Viewer
    Location:
    1p35.3
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    109 current GRCh38.p12 (GCF_000001405.38) 1 NC_000001.11 (27830741..27851672)
    105 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (28157252..28178183)

    Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene syntaxin 12 Neighboring gene RNA, U6 small nuclear 1245, pseudogene Neighboring gene small Cajal body-specific RNA 1 Neighboring gene thymocyte selection associated family member 2 Neighboring gene replication protein A2

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    Knockdown of protein phosphatase 1, regulatory subunit 8 (PPP1R8) by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat PP1 interacts with Tat in part through the binding of Val(36) and Phe(38) of Tat to PP1, and Tat is involved in the nuclear and subnuclear targeting of PP1 PubMed
    tat Nuclear inhibitor of PP1 (NIPP1) inhibits HIV-1 Tat-induced transcription, an effect that can be reversed by co-expression of PP1, indicating Tat function is regulated by PP1/NIPP1 PubMed

    Go to the HIV-1, Human Interaction Database

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    DNA binding IEA
    Inferred from Electronic Annotation
    more info
     
    RNA binding TAS
    Traceable Author Statement
    more info
    PubMed 
    endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
     
    mRNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    protein serine/threonine phosphatase inhibitor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    ribonuclease E activity TAS
    Traceable Author Statement
    more info
    PubMed 
    Process Evidence Code Pubs
    RNA catabolic process TAS
    Traceable Author Statement
    more info
    PubMed 
    RNA phosphodiester bond hydrolysis IEA
    Inferred from Electronic Annotation
    more info
     
    RNA splicing IEA
    Inferred from Electronic Annotation
    more info
     
    cell proliferation IEA
    Inferred from Electronic Annotation
    more info
     
    mRNA processing IEA
    Inferred from Electronic Annotation
    more info
     
    negative regulation of phosphoprotein phosphatase activity IEA
    Inferred from Electronic Annotation
    more info
     
    negative regulation of protein dephosphorylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    production of miRNAs involved in gene silencing by miRNA IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    Component Evidence Code Pubs
    cytoplasm IEA
    Inferred from Electronic Annotation
    more info
     
    nuclear speck IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    nuclear speck IDA
    Inferred from Direct Assay
    more info
    PubMed 
    nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    spliceosomal complex IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    nuclear inhibitor of protein phosphatase 1
    Names
    RNase E
    activator of RNA decay
    nuclear inhibitor of protein phosphatase-1 alpha
    nuclear inhibitor of protein phosphatase-1 beta
    nuclear subunit of PP-1
    protein phosphatase 1, regulatory (inhibitor) subunit 8

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_002713.3NP_002704.1  nuclear inhibitor of protein phosphatase 1 isoform gamma

      See identical proteins and their annotated locations for NP_002704.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) encodes the shortest isoform (gamma, also called ARD-1). It has its first exon longer at the 3' end and lacks an internal exon as compared to that of transcript variant 1. The gamma isoform uses a different translation start site and is N-terminally truncated as compared to isoform alpha encoded by variant 1. Isoform gamma contains ribonuclease activity.
      Source sequence(s)
      AF061959, DA077576, U14575
      Consensus CDS
      CCDS313.1
      UniProtKB/Swiss-Prot
      Q12972
      UniProtKB/TrEMBL
      Q6ICT4
      Related
      ENSP00000236412.7, ENST00000236412.11
    2. NM_014110.5NP_054829.2  nuclear inhibitor of protein phosphatase 1 isoform alpha

      See identical proteins and their annotated locations for NP_054829.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) is the shortest transcript but encodes the predominant and longest isoform (alpha). Isoform alpha contains protein phosphatase inhibitory actvity.
      Source sequence(s)
      AF061959, AK292077, AL109927, DA167043
      Consensus CDS
      CCDS311.1
      UniProtKB/Swiss-Prot
      Q12972
      Related
      ENSP00000311677.5, ENST00000311772.10
      Conserved Domains (2) summary
      cd00060
      Location:27119
      FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
      pfam11945
      Location:98212
      WASH_WAHD; WAHD domain of WASH complex
    3. NM_138558.2NP_612568.1  nuclear inhibitor of protein phosphatase 1 isoform beta/delta

      See identical proteins and their annotated locations for NP_612568.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) is the longest transcript and has its first exon longer at the 3' end as compared to that of transcript variant 1. The resulting isoform (beta/delta) uses a different translation start site and is N-terminally truncated as compared to isoform alpha encoded by variant 1. Isoform beta/delta contains protein phosphatase inhibitory activity.
      Source sequence(s)
      AF061959, DA693246
      Consensus CDS
      CCDS312.1
      UniProtKB/Swiss-Prot
      Q12972
      Related
      ENSP00000363042.4, ENST00000373931.8

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p12 Primary Assembly

    Genomic

    1. NC_000001.11 Reference GRCh38.p12 Primary Assembly

      Range
      27830741..27851672
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017001594.2XP_016857083.1  nuclear inhibitor of protein phosphatase 1 isoform X1

      UniProtKB/Swiss-Prot
      Q12972
      UniProtKB/TrEMBL
      Q6ICT4
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