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    BRD4 bromodomain containing 4 [ Homo sapiens (human) ]

    Gene ID: 23476, updated on 12-Aug-2018

    Summary

    Official Symbol
    BRD4provided by HGNC
    Official Full Name
    bromodomain containing 4provided by HGNC
    Primary source
    HGNC:HGNC:13575
    See related
    Ensembl:ENSG00000141867 MIM:608749; Vega:OTTHUMG00000183252
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    CAP; MCAP; HUNK1; HUNKI
    Summary
    The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in placenta (RPKM 10.8), testis (RPKM 9.2) and 25 other tissues See more
    Orthologs

    Genomic context

    See BRD4 in Genome Data Viewer
    Location:
    19p13.12
    Exon count:
    23
    Annotation release Status Assembly Chr Location
    109 current GRCh38.p12 (GCF_000001405.38) 19 NC_000019.10 (15236836..15332543, complement)
    105 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (15348301..15443354, complement)

    Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene notch 3 Neighboring gene microRNA 6795 Neighboring gene epoxide hydrolase 3 Neighboring gene A-kinase anchoring protein 8 Neighboring gene A-kinase anchoring protein 8 like

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Jun 15 11:32:44 2016

    Bibliography

    GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    HIV-1 replication is inhibited by BRD4; siRNA-mediated knockdown allows for Tat-induced latency reversal PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat The RxL or KxL motif and the polybasic cluster of HIV-1 Tat and BRD4 exhibit similar importance for P-TEFb activation PubMed
    tat The C-terminal 34 residues (1329-1362) of BRD4 are crucial for interaction with P-TEFb. Overexpression of the BRD4 P-TEFb-interacting domain disrupts the interaction between HIV-1 Tat and P-TEFb PubMed
    tat CDK9-CycT1-AFF1 is stimulated by HIV-1 Tat and transferred as a single complex unit to BRD4 upon stress-induced disruption of AFF1-containing 7SK snRNP (HEXIM1, MEPCE, LARP7, 7SK RNA, CDK9, CycT1, and AFF1) PubMed
    tat Phosphorylation of CDK9 at position Ser175 regulates the competition between HIV-1 Tat and BRD4 for P-TEFb binding PubMed
    tat Brd4 inhibits HIV-1 Tat-human super elongation complex (components AFF4, ELL2, CycT1, and CDK9) by competing with HIV-1 Tat for binding to P-TEFb on HIV-1 promoter PubMed
    tat JQ1, a small molecule inhibitor of Brd4, directly dissociates Brd4 from HIV-1 promoter and promotes subsequently HIV-1 Tat binding to HIV-1 promoter, resulting in increased phosphorylation of RNA polymerase II and viral transcription PubMed

    Go to the HIV-1, Human Interaction Database

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    RNA polymerase II C-terminal domain binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    RNA polymerase II CTD heptapeptide repeat kinase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    chromatin binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enzyme binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    lysine-acetylated histone binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    p53 binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    cellular response to DNA damage stimulus IEA
    Inferred from Electronic Annotation
    more info
     
    chromatin organization IEA
    Inferred from Electronic Annotation
    more info
     
    positive regulation of G2/M transition of mitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of I-kappaB kinase/NF-kappaB signaling IDA
    Inferred from Direct Assay
    more info
    PubMed 
    positive regulation of histone H3-K36 trimethylation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    PubMed 
    positive regulation of transcription elongation from RNA polymerase II promoter IDA
    Inferred from Direct Assay
    more info
    PubMed 
    positive regulation of transcription elongation from RNA polymerase II promoter IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of transcription, DNA-templated IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    protein phosphorylation IEA
    Inferred from Electronic Annotation
    more info
     
    regulation of inflammatory response IDA
    Inferred from Direct Assay
    more info
    PubMed 
    regulation of phosphorylation of RNA polymerase II C-terminal domain IDA
    Inferred from Direct Assay
    more info
    PubMed 
    regulation of transcription involved in G1/S transition of mitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    transcription, DNA-templated IEA
    Inferred from Electronic Annotation
    more info
     
    viral process IEA
    Inferred from Electronic Annotation
    more info
     
    Component Evidence Code Pubs
    condensed nuclear chromosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytosol IDA
    Inferred from Direct Assay
    more info
     
    nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    bromodomain-containing protein 4
    Names
    chromosome-associated protein
    NP_001317313.1
    NP_055114.1
    NP_490597.1
    XP_011526156.1
    XP_011526158.1

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001330384.1NP_001317313.1  bromodomain-containing protein 4 isoform 3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (short) differs in the 3' UTR and coding region compared to variant long. The resulting isoform (short) is shorter and has a distinct C-terminus compared to isoform long.
      Source sequence(s)
      AC005776, BC035266
      Consensus CDS
      CCDS82307.1
      UniProtKB/Swiss-Prot
      O60885
      UniProtKB/TrEMBL
      A0A024R7H8
      Related
      ENSP00000353112.4, ENST00000360016.9
      Conserved Domains (3) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
    2. NM_014299.2NP_055114.1  bromodomain-containing protein 4 isoform short

      See identical proteins and their annotated locations for NP_055114.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (short) differs in the 3' UTR and coding region compared to variant long. The resulting isoform (short) is shorter and has a distinct C-terminus compared to isoform long.
      Source sequence(s)
      AC004798, BC035266, Y12059
      Consensus CDS
      CCDS46004.1
      UniProtKB/Swiss-Prot
      O60885
      Related
      ENSP00000360901.3, OTTHUMP00000271251, ENST00000371835.8, OTTHUMT00000465801
      Conserved Domains (3) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
    3. NM_058243.2NP_490597.1  bromodomain-containing protein 4 isoform long

      See identical proteins and their annotated locations for NP_490597.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (long) encodes the full length isoform.
      Source sequence(s)
      AF386649, BC035266
      Consensus CDS
      CCDS12328.1
      UniProtKB/Swiss-Prot
      O60885
      Related
      ENSP00000263377.1, OTTHUMP00000271250, ENST00000263377.6, OTTHUMT00000465800
      Conserved Domains (6) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
      pfam17105
      Location:13211362
      BRD4_CDT; C-terminal domain of bromodomain protein 4
      cl25742
      Location:12301271
      ERM; Ezrin/radixin/moesin family
      cl27169
      Location:809922
      ZipA; ZipA C-terminal domain. ZipA, a membrane-anchored protein, is one of at least nine essential gene products necessary for assembly of the septal ring which mediates cell division in E.coli. ZipA and FtsA directly bind FtsZ, a homolog of eukaryotic ...

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109 details...Open this link in a new tab

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p12 Primary Assembly

    Genomic

    1. NC_000019.10 Reference GRCh38.p12 Primary Assembly

      Range
      15236836..15332543 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011527854.2XP_011526156.1  bromodomain-containing protein 4 isoform X1

      See identical proteins and their annotated locations for XP_011526156.1

      UniProtKB/Swiss-Prot
      O60885
      Conserved Domains (6) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
      pfam17105
      Location:13211362
      BRD4_CDT; C-terminal domain of bromodomain protein 4
      cl25742
      Location:12301271
      ERM; Ezrin/radixin/moesin family
      cl27169
      Location:809922
      ZipA; ZipA C-terminal domain. ZipA, a membrane-anchored protein, is one of at least nine essential gene products necessary for assembly of the septal ring which mediates cell division in E.coli. ZipA and FtsA directly bind FtsZ, a homolog of eukaryotic ...
    2. XM_011527856.3XP_011526158.1  bromodomain-containing protein 4 isoform X2

      See identical proteins and their annotated locations for XP_011526158.1

      UniProtKB/Swiss-Prot
      O60885
      Conserved Domains (3) summary
      cd05497
      Location:58164
      Bromo_Brdt_I_like; Bromodomain, Brdt_like subfamily, repeat I. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      cd05498
      Location:353454
      Bromo_Brdt_II_like; Bromodomain, Brdt_like subfamily, repeat II. Human Brdt is a testis-specific member of the BET subfamily of bromodomain proteins; the first bromodomain in Brdt has been shown to be essential for male germ cell differentiation. Bromodomains are 110 amino ...
      pfam17035
      Location:609673
      BET; Bromodomain extra-terminal - transcription regulation
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