TRIM35 tripartite motif containing 35 [Homo sapiens (human)] - Gene

Summary

Official Symbol: TRIM35provided by HGNC
Official Full Name: tripartite motif containing 35provided by HGNC
Primary source: HGNC:HGNC:16285
See related: Ensembl:ENSG00000104228 MIM:617007; AllianceGenome:HGNC:16285
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: HLS5; MAIR
Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in skin (RPKM 5.7), testis (RPKM 5.1) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 8p21.2

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	8	NC_000008.11 (27284886..27311272, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	8	NC_060932.1 (27561710..27588095, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	8	NC_000008.10 (27142403..27168789, complement)

Chromosome 8 - NC_000008.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

IRF5 promotes glycolysis in the progression of hepatocellular carcinoma and is regulated by TRIM35.
Title: IRF5 promotes glycolysis in the progression of hepatocellular carcinoma and is regulated by TRIM35.
Inhibition of human adenovirus replication by TRIM35-mediated degradation of E1A.
Title: Inhibition of human adenovirus replication by TRIM35-mediated degradation of E1A.
TRIM35 ubiquitination regulates the expression of PKM2 tetramer and dimer and affects the malignant behaviour of breast cancer by regulating the Warburg effect.
Title: TRIM35 ubiquitination regulates the expression of PKM2 tetramer and dimer and affects the malignant behaviour of breast cancer by regulating the Warburg effect.
TRIM35 functions as a novel tumor suppressor in breast cancer by inducing cell apoptosis through ubiquitination of PDK1.
Title: TRIM35 functions as a novel tumor suppressor in breast cancer by inducing cell apoptosis through ubiquitination of PDK1.
Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration.
Title: Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration.
Tripartite motif containing 35 contributes to the proliferation, migration, and invasion of lung cancer cells in vitro and in vivo.
Title: Tripartite motif containing 35 contributes to the proliferation, migration, and invasion of lung cancer cells in vitro and in vivo.
Authors subsequently investigated whether miR-4417 and TRIM35 regulate HCC cell proliferation and apoptosis through PKM2 Y105 phosphorylation, and the results supported our speculation that miR-4417 targets TRIM35 and regulates the Y105 phosphorylation of PKM2 to promote hepatocarcinogenesis.
Title: miR-4417 Targets Tripartite Motif-Containing 35 (TRIM35) and Regulates Pyruvate Kinase Muscle 2 (PKM2) Phosphorylation to Promote Proliferation and Suppress Apoptosis in Hepatocellular Carcinoma Cells.
Data suggest that pyruvate kinase isoform M2 (PKM2)/tripartite motif-containing 35 (TRIM35) expression could be a biomarker for the prognosis of hepatocellular carcinoma (HCC) and target for cancer therapy.
Title: Co-expression of PKM2 and TRIM35 predicts survival and recurrence in hepatocellular carcinoma.
TRIM35 regulates the Warburg effect and tumorigenicity through interaction with PKM2 in hepatocellular carcinoma.
Title: TRIM35 Interacts with pyruvate kinase isoform M2 to suppress the Warburg effect and tumorigenicity in hepatocellular carcinoma.
gene located on chromosome 8p21, a region implicated in leukemias and solid tumors; enforced expression inhibited cell growth, clonogenicity, and tumorigenicity; conceivable HLS5 is one of the tumor suppressor genes thought to reside at the 8p21 locus
Title: HLS5, a novel RBCC (ring finger, B box, coiled-coil) family member isolated from a hemopoietic lineage switch, is a candidate tumor suppressor.

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Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	HIV-1 gp120-treated vaginal epithelial cells show downregulation of tripartite motif containing 35 (TRIM35) expression as compared to untreated control	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

A009Z35 (e-PCR)
- UniSTS:66311

RH103993 (e-PCR)
- UniSTS:98318

RH67896 (e-PCR)
- UniSTS:44830

RH46973 (e-PCR)
- UniSTS:31804

G33026 (e-PCR)
- UniSTS:117603

SGC33557 (e-PCR)
- UniSTS:82282

RH35960 (e-PCR)
- UniSTS:81779

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in innate immune response	IBA Inferred from Biological aspect of Ancestor more info
involved_in innate immune response	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in suppression of viral release by host	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase TRIM35

Names: hemopoietic lineage switch protein 5; tripartite motif-containing protein 35

NP_001291424.1

EC 2.3.2.27

NP_001349742.1

EC 2.3.2.27

NP_741983.2

EC 2.3.2.27

XP_047277558.1

EC 2.3.2.27

XP_054216142.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001304495.2 → NP_001291424.1 E3 ubiquitin-protein ligase TRIM35 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks two exons in the central coding region one of which results in a frameshift compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC124649, AK308236, BU622420

Consensus CDS

CCDS78322.1

UniProtKB/TrEMBL

E5RGB3

Related

ENSP00000428770.1, ENST00000521253.1

Conserved Domains (2) summary

cd00021
Location:99 → 137

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cl17238
Location:18 → 62

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
NM_001362813.2 → NP_001349742.1 E3 ubiquitin-protein ligase TRIM35 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an alternate exon in the central coding region, resulting in a frameshift compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC124649, AF492463, BU622420

Conserved Domains (4) summary

COG1322
Location:133 → 246

RmuC; DNA anti-recombination protein (rearrangement mutator) RmuC [Replication, recombination and repair]

cd00162
Location:21 → 60

RING_Ubox; RING-HC finger (C3HC4-type) [structural motif]

cl00034
Location:96 → 139

Bbox_SF; B-box-type zinc finger superfamily

cl17238
Location:18 → 62

RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
NM_171982.5 → NP_741983.2 E3 ubiquitin-protein ligase TRIM35 isoform 1

See identical proteins and their annotated locations for NP_741983.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AC124649, AF492463, BU622420

Consensus CDS

CCDS6056.2

UniProtKB/Swiss-Prot

Q86XQ0, Q8WVA4, Q9UPQ4

Related

ENSP00000301924.4, ENST00000305364.9

Conserved Domains (4) summary

cd12893
Location:303 → 482

SPRY_PRY_TRIM35; PRY/SPRY domain in tripartite motif-containing protein 35 (TRIM35)

cd16599
Location:18 → 62

RING-HC_TRIM35_C-IV; RING finger, HC subclass, found in tripartite motif-containing protein 35 (TRIM35) and similar proteins

pfam13863
Location:151 → 247

DUF4200; Domain of unknown function (DUF4200)

cl00034
Location:96 → 139

Bbox_SF; B-box-type zinc finger superfamily