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    DROSHA drosha ribonuclease III [ Homo sapiens (human) ]

    Gene ID: 29102, updated on 10-Feb-2018
    Official Symbol
    DROSHAprovided by HGNC
    Official Full Name
    drosha ribonuclease IIIprovided by HGNC
    Primary source
    HGNC:HGNC:17904
    See related
    Ensembl:ENSG00000113360 MIM:608828; Vega:OTTHUMG00000161976
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
    Summary
    This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]
    Expression
    Ubiquitous expression in brain (RPKM 9.5), ovary (RPKM 7.6) and 25 other tissues See more
    Orthologs
    Location:
    5p13.3
    Exon count:
    38
    Annotation release Status Assembly Chr Location
    108 current GRCh38.p7 (GCF_000001405.33) 5 NC_000005.10 (31400494..31532175, complement)
    105 previous assembly GRCh37.p13 (GCF_000001405.25) 5 NC_000005.9 (31400601..31532282, complement)

    Chromosome 5 - NC_000005.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105374709 Neighboring gene cadherin 6 Neighboring gene double homeobox 4 like 51 (pseudogene) Neighboring gene chromosome 5 open reading frame 22 Neighboring gene RNA, U6 small nuclear 363, pseudogene Neighboring gene PDZ domain containing 2 Neighboring gene RNA, U6 small nuclear 358, pseudogene

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Jun 15 11:32:44 2016

    GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

    Protein interactions

    Protein Gene Interaction Pubs
    Pr55(Gag) gag Knockdown of Drosha by shRNA leads to inhibit enrichment of HIV-1 Gag in intracellular vacuoles, but enhance viral particle release in Gag expressing cells PubMed

    Go to the HIV-1, Human Interaction Database

    • DDX1 as a regulatory component of the Drosha microprocessor, organism-specific biosystem (from WikiPathways)
      DDX1 as a regulatory component of the Drosha microprocessor, organism-specific biosystemPosttranscriptional maturation is a critical step in microRNA (miRNA) biogenesis that determines mature miRNA levels. In addition to core components (Drosha and DGCR8 [DiGeorge syndrome critical regi...
    • Direct p53 effectors, organism-specific biosystem (from Pathway Interaction Database)
      Direct p53 effectors, organism-specific biosystem
      Direct p53 effectors
    • Gene Expression, organism-specific biosystem (from REACTOME)
      Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
    • Gene Silencing by RNA, organism-specific biosystem (from REACTOME)
      Gene Silencing by RNA, organism-specific biosystemIn this module, the biology of various types of regulatory non-coding RNAs are described. Currently, biogenesis and functions of small interfering RNAs (siRNAs) and microRNAs are annotated.
    • MicroRNA (miRNA) biogenesis, organism-specific biosystem (from REACTOME)
      MicroRNA (miRNA) biogenesis, organism-specific biosystemBiogenesis of microRNAs (miRNAs) can be summarized in five steps (reviewed in Ketting 2011, Nowotny and Yang 2009, Kim et al. 2009, Chua et al. 2009, Hannon and He 2004):1. Transcription. miRNA trans...
    • Proteoglycans in cancer, organism-specific biosystem (from KEGG)
      Proteoglycans in cancer, organism-specific biosystemMany proteoglycans (PGs) in the tumor microenvironment have been shown to be key macromolecules that contribute to biology of various types of cancer including proliferation, adhesion, angiogenesis a...
    • Proteoglycans in cancer, conserved biosystem (from KEGG)
      Proteoglycans in cancer, conserved biosystemMany proteoglycans (PGs) in the tumor microenvironment have been shown to be key macromolecules that contribute to biology of various types of cancer including proliferation, adhesion, angiogenesis a...
    • RNA interference, organism-specific biosystem (from WikiPathways)
      RNA interference, organism-specific biosystemThe process of RNA-interference in eukaryotic cells. Long precursor microRNA (miRNA), called pri-miRNA, is cleaved by RNase III endonuclease (Drosha) into pieces of approximately 70 nucleotides each ...
    • Ribosome biogenesis in eukaryotes, organism-specific biosystem (from KEGG)
      Ribosome biogenesis in eukaryotes, organism-specific biosystemRibosomes are the cellular factories responsible for making proteins. In eukaryotes, ribosome biogenesis involves the production and correct assembly of four rRNAs and about 80 ribosomal proteins. It...
    • Ribosome biogenesis in eukaryotes, conserved biosystem (from KEGG)
      Ribosome biogenesis in eukaryotes, conserved biosystemRibosomes are the cellular factories responsible for making proteins. In eukaryotes, ribosome biogenesis involves the production and correct assembly of four rRNAs and about 80 ribosomal proteins. It...
    • miRNA Biogenesis, organism-specific biosystem (from WikiPathways)
      miRNA Biogenesis, organism-specific biosystemmicroRNA biogenesis + an example of a miRBase entry. As an example immature hsa-mir-146a is shown
    Products Interactant Other Gene Complex Source Pubs Description

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    DEAD/H-box RNA helicase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    R-SMAD binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    RNA binding HDA PubMed 
    SMAD binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    double-stranded RNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    lipopolysaccharide binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    primary miRNA binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    primary miRNA binding NAS
    Non-traceable Author Statement
    more info
    PubMed 
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    ribonuclease III activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Process Evidence Code Pubs
    RNA phosphodiester bond hydrolysis, endonucleolytic IEA
    Inferred from Electronic Annotation
    more info
     
    defense response to Gram-negative bacterium IDA
    Inferred from Direct Assay
    more info
    PubMed 
    defense response to Gram-positive bacterium IDA
    Inferred from Direct Assay
    more info
    PubMed 
    miRNA metabolic process TAS
    Traceable Author Statement
    more info
     
    positive regulation of gene expression IEA
    Inferred from Electronic Annotation
    more info
     
    pre-miRNA processing IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    primary miRNA processing IDA
    Inferred from Direct Assay
    more info
    PubMed 
    primary miRNA processing IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    production of siRNA involved in RNA interference IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    rRNA catabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    regulation of inflammatory response IEA
    Inferred from Electronic Annotation
    more info
     
    regulation of miRNA metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    regulation of regulatory T cell differentiation IEA
    Inferred from Electronic Annotation
    more info
     
    ribosome biogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    Component Evidence Code Pubs
    microprocessor complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    nucleolus IEA
    Inferred from Electronic Annotation
    more info
     
    nucleoplasm TAS
    Traceable Author Statement
    more info
     
    postsynaptic density IEA
    Inferred from Electronic Annotation
    more info
     
    Preferred Names
    ribonuclease 3
    Names
    RNase III
    drosha, double-stranded RNA-specific endoribonuclease
    nuclear RNase III Drosha
    p241
    putative protein p241 which interacts with transcription factor Sp1
    putative ribonuclease III
    ribonuclease type III, nuclear
    NP_001093882.1
    NP_037367.3
    XP_005248348.1
    XP_005248349.1
    XP_005248350.1
    XP_005248351.1
    XP_011512335.1
    XP_016864888.1
    XP_016864889.1
    XP_016864890.1
    XP_016864891.1

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_051574.1 RefSeqGene

      Range
      5001..136682
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001100412.1NP_001093882.1  ribonuclease 3 isoform 2

      See identical proteins and their annotated locations for NP_001093882.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1.
      Source sequence(s)
      AA917625, AC008768, BC024261, BX647724, DB065470
      Consensus CDS
      CCDS47194.1
      UniProtKB/Swiss-Prot
      Q9NRR4
      Related
      ENSP00000424161.1, OTTHUMP00000220912, ENST00000513349.5, OTTHUMT00000366562
      Conserved Domains (3) summary
      cd00593
      Location:9221042
      RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
      pfam08648
      Location:230357
      DUF1777; Protein of unknown function (DUF1777)
      cl25983
      Location:10681296
      Rnc; dsRNA-specific ribonuclease [Transcription]
    2. NM_013235.4NP_037367.3  ribonuclease 3 isoform 1

      See identical proteins and their annotated locations for NP_037367.3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AA917625, AC008768, AF189011, BC024261, DB065470
      Consensus CDS
      CCDS47195.1
      UniProtKB/Swiss-Prot
      Q9NRR4
      Related
      ENSP00000425979.2, OTTHUMP00000220911, ENST00000511367.6, OTTHUMT00000366561
      Conserved Domains (5) summary
      smart00358
      Location:12611332
      DSRM; Double-stranded RNA binding motif
      PRK00102
      Location:11051333
      rnc; ribonuclease III; Reviewed
      cd00593
      Location:11221254
      RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
      pfam08648
      Location:217310
      DUF1777; Protein of unknown function (DUF1777)
      pfam12871
      Location:219278
      PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 108

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p7 Primary Assembly

    Genomic

    1. NC_000005.10 Reference GRCh38.p7 Primary Assembly

      Range
      31400494..31532175 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017009401.1XP_016864890.1  ribonuclease 3 isoform X7

    2. XM_005248294.3XP_005248351.1  ribonuclease 3 isoform X6

      See identical proteins and their annotated locations for XP_005248351.1

      UniProtKB/Swiss-Prot
      Q9NRR4
      Conserved Domains (5) summary
      smart00358
      Location:11931264
      DSRM; Double-stranded RNA binding motif
      PRK00102
      Location:10371265
      rnc; ribonuclease III; Reviewed
      cd00593
      Location:10541186
      RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
      pfam08648
      Location:219332
      DUF1777; Protein of unknown function (DUF1777)
      pfam12871
      Location:218296
      PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term
    3. XM_017009399.1XP_016864888.1  ribonuclease 3 isoform X4

    4. XM_005248293.3XP_005248350.1  ribonuclease 3 isoform X3

      Conserved Domains (5) summary
      smart00358
      Location:12301301
      DSRM; Double-stranded RNA binding motif
      PRK00102
      Location:10741302
      rnc; ribonuclease III; Reviewed
      cd00593
      Location:10911223
      RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
      pfam08648
      Location:219286
      DUF1777; Protein of unknown function (DUF1777)
      pfam12871
      Location:219280
      PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term
    5. XM_017009400.1XP_016864889.1  ribonuclease 3 isoform X5

      Conserved Domains (3) summary
      cd00593
      Location:9141034
      RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
      pfam08648
      Location:230357
      DUF1777; Protein of unknown function (DUF1777)
      cl25983
      Location:10601288
      Rnc; dsRNA-specific ribonuclease [Transcription]
    6. XM_005248292.3XP_005248349.1  ribonuclease 3 isoform X2

      Conserved Domains (5) summary
      smart00358
      Location:12531324
      DSRM; Double-stranded RNA binding motif
      PRK00102
      Location:10971325
      rnc; ribonuclease III; Reviewed
      cd00593
      Location:11141246
      RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
      pfam08648
      Location:217310
      DUF1777; Protein of unknown function (DUF1777)
      pfam12871
      Location:219278
      PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term
    7. XM_005248291.3XP_005248348.1  ribonuclease 3 isoform X1

      See identical proteins and their annotated locations for XP_005248348.1

      UniProtKB/Swiss-Prot
      Q9NRR4
      Conserved Domains (5) summary
      smart00358
      Location:12611332
      DSRM; Double-stranded RNA binding motif
      PRK00102
      Location:11051333
      rnc; ribonuclease III; Reviewed
      cd00593
      Location:11221254
      RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
      pfam08648
      Location:217310
      DUF1777; Protein of unknown function (DUF1777)
      pfam12871
      Location:219278
      PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term
    8. XM_017009402.1XP_016864891.1  ribonuclease 3 isoform X9

    9. XM_011514033.2XP_011512335.1  ribonuclease 3 isoform X8

      Conserved Domains (3) summary
      cd00593
      Location:9591079
      RIBOc; RIBOc. Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in ...
      pfam08648
      Location:217310
      DUF1777; Protein of unknown function (DUF1777)
      pfam12871
      Location:219278
      PRP38_assoc; Pre-mRNA-splicing factor 38-associated hydrophilic C-term

    Alternate CHM1_1.1

    Genomic

    1. NC_018916.2 Alternate CHM1_1.1

      Range
      31402053..31533647 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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