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    PKM pyruvate kinase, muscle [ Homo sapiens (human) ]

    Gene ID: 5315, updated on 23-Apr-2017
    Official Symbol
    PKMprovided by HGNC
    Official Full Name
    pyruvate kinase, muscleprovided by HGNC
    Primary source
    HGNC:HGNC:9021
    See related
    Ensembl:ENSG00000067225 MIM:179050; Vega:OTTHUMG00000172709
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
    Summary
    This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
    Orthologs
    Location:
    15q23
    Exon count:
    16
    Annotation release Status Assembly Chr Location
    108 current GRCh38.p7 (GCF_000001405.33) 15 NC_000015.10 (72199029..72231624, complement)
    105 previous assembly GRCh37.p13 (GCF_000001405.25) 15 NC_000015.9 (72491370..72523901, complement)

    Chromosome 15 - NC_000015.10Genomic Context describing neighboring genes Neighboring gene SUMO/sentrin peptidase family member, NEDD8 specific Neighboring gene ribosomal protein L21 pseudogene 115 Neighboring gene uncharacterized LOC107984715 Neighboring gene GRAM domain containing 2 Neighboring gene poly(ADP-ribose) polymerase family member 6 Neighboring gene uncharacterized LOC105370887

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Jun 15 11:32:44 2016

    GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify pyruvate kinase of muscle (PKM), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 is identified to have a physical interaction with pyruvate kinase, muscle (PKM2) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed
    Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify pyruvate kinase of muscle (PKM), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Nef nef Tandem affinity purification and mass spectrometry analysis identify pyruvate kinase of muscle (PKM), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify pyruvate kinase of muscle (PKM), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Tat tat Expression of HIV-1 Tat upregulates the abundance of pyruvate kinase, muscle (PKM) in the nucleoli of Jurkat T-cells PubMed
    Vpr vpr A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies upregulation of pyruvate kinase, muscle (PKM) expression by HIV-1 Vpr in Vpr transduced macrophages PubMed
    retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human pyruvate kinase, muscle (PKM) at amino acid residues 306-307 and 359-361 by the HIV-1 protease PubMed

    Go to the HIV-1, Human Interaction Database

    Products Interactant Other Gene Complex Source Pubs Description

    Markers

    Homology

    Clone Names

    • MGC3932

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    MHC class II protein complex binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    RNA binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cadherin binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    potassium ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    pyruvate kinase activity EXP
    Inferred from Experiment
    more info
    PubMed 
    pyruvate kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    pyruvate kinase activity TAS
    Traceable Author Statement
    more info
    PubMed 
    Process Evidence Code Pubs
    canonical glycolysis TAS
    Traceable Author Statement
    more info
     
    cellular response to insulin stimulus IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    neutrophil degranulation TAS
    Traceable Author Statement
    more info
     
    programmed cell death IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Component Evidence Code Pubs
    cilium IEA
    Inferred from Electronic Annotation
    more info
     
    cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytosol IDA
    Inferred from Direct Assay
    more info
     
    cytosol NAS
    Non-traceable Author Statement
    more info
    PubMed 
    cytosol TAS
    Traceable Author Statement
    more info
     
    extracellular exosome IDA
    Inferred from Direct Assay
    more info
    PubMed 
    extracellular matrix IDA
    Inferred from Direct Assay
    more info
    PubMed 
    extracellular region TAS
    Traceable Author Statement
    more info
     
    extracellular vesicle IDA
    Inferred from Direct Assay
    more info
    PubMed 
    ficolin-1-rich granule lumen TAS
    Traceable Author Statement
    more info
     
    mitochondrion IDA
    Inferred from Direct Assay
    more info
    PubMed 
    myelin sheath IEA
    Inferred from Electronic Annotation
    more info
     
    nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    secretory granule lumen TAS
    Traceable Author Statement
    more info
     
    vesicle IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Preferred Names
    pyruvate kinase PKM
    Names
    OIP-3
    OPA-interacting protein 3
    PK, muscle type
    cytosolic thyroid hormone-binding protein
    epididymis secretory protein Li 30
    p58
    pyruvate kinase 2/3
    pyruvate kinase isozymes M1/M2
    pyruvate kinase muscle isozyme
    thyroid hormone-binding protein 1
    thyroid hormone-binding protein, cytosolic
    tumor M2-PK
    NP_001193725.1
    NP_001193726.1
    NP_001193727.1
    NP_001193728.1
    NP_001303247.1
    NP_002645.3
    NP_872270.1
    NP_872271.1

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_052978.1 RefSeqGene

      Range
      5001..37596
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001206796.2NP_001193725.1  pyruvate kinase PKM isoform c

      See identical proteins and their annotated locations for NP_001193725.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (c).
      Source sequence(s)
      AC020779, AK294315, BC000481, BC035198, DA382029, DC326232, DC376054
      UniProtKB/Swiss-Prot
      P14618
      Conserved Domains (2) summary
      cd00288
      Location:116604
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:117601
      pyruv_kin; pyruvate kinase
    2. NM_001206797.2NP_001193726.1  pyruvate kinase PKM isoform d

      See identical proteins and their annotated locations for NP_001193726.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence, lacks an alternate in-frame segment, and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (d) is shorter at the N-terminus, lacks an alternate internal segment, and has a different internal segment compared to isoform c.
      Source sequence(s)
      AC020779, AK297951, BC000481, BC035198, DC326232, DC376054
      Consensus CDS
      CCDS73752.1
      UniProtKB/Swiss-Prot
      P14618
      UniProtKB/TrEMBL
      B4DNK4
      Related
      ENSP00000373745.4, ENST00000389093.7
      Conserved Domains (2) summary
      cd00288
      Location:42456
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:43453
      pyruv_kin; pyruvate kinase
    3. NM_001206798.2NP_001193727.1  pyruvate kinase PKM isoform e

      See identical proteins and their annotated locations for NP_001193727.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence, lacks an alternate exon, and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (e) has a shorter and distinct N-terminus, lacks an alternate internal segment, and has a different internal segment compared to isoform c.
      Source sequence(s)
      AC020779, AK300800, BC000481, BC035198, DC326232, DC376054
      Consensus CDS
      CCDS55972.1
      UniProtKB/Swiss-Prot
      P14618
      Related
      ENSP00000403365.2, ENST00000449901.6
      Conserved Domains (2) summary
      cd00288
      Location:68515
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:68512
      pyruv_kin; pyruvate kinase
    4. NM_001206799.1NP_001193728.1  pyruvate kinase PKM isoform f

      See identical proteins and their annotated locations for NP_001193728.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (7) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (f) has a shorter and distinct N-terminus compared to isoform c.
      Source sequence(s)
      AK294315, AK309781, BC000481, BC035198
      UniProtKB/Swiss-Prot
      P14618
      Conserved Domains (2) summary
      cd00288
      Location:47535
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:48532
      pyruv_kin; pyruvate kinase
    5. NM_001316318.1NP_001303247.1  pyruvate kinase PKM isoform g

      Status: REVIEWED

      Description
      Transcript Variant: This variant (8) lacks an alternate in-frame exon in the 5' end of the coding sequence compared to variant 4. The resulting isoform (g) has the same N- and C-termini but is shorter compared to isoform c.
      Source sequence(s)
      AC020779, AK294315, BC000481, BC035198, BC096823, DC326232, DC376054
      UniProtKB/Swiss-Prot
      P14618
      Conserved Domains (2) summary
      cd00288
      Location:77565
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:78562
      pyruv_kin; pyruvate kinase
    6. NM_002654.5NP_002645.3  pyruvate kinase PKM isoform a

      See identical proteins and their annotated locations for NP_002645.3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence and has an alternate in-frame coding exon compared to variant 4. The resulting isoform (a, also called M2) is shorter at the N-terminus and has a different internal segment compared to isoform c.
      Source sequence(s)
      AC020779, BC000481, BC035198, DC326232, DC376054
      Consensus CDS
      CCDS32284.1
      UniProtKB/Swiss-Prot
      P14618
      UniProtKB/TrEMBL
      V9HWB8
      Related
      ENSP00000334983.5, OTTHUMP00000249141, ENST00000335181.9, OTTHUMT00000420056
      Conserved Domains (2) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:43527
      pyruv_kin; pyruvate kinase
    7. NM_182470.3NP_872270.1  pyruvate kinase PKM isoform b

      See identical proteins and their annotated locations for NP_872270.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (b, also called M1) is shorter at the N-terminus compared to isoform c.
      Source sequence(s)
      AC020779, AK092369, BC023592, BC035198, DC326232, DC376054
      Consensus CDS
      CCDS32285.1
      UniProtKB/Swiss-Prot
      P14618
      UniProtKB/TrEMBL
      A0A024R5Z9
      Related
      ENSP00000320171.6, ENST00000319622.10
      Conserved Domains (2) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:43527
      pyruv_kin; pyruvate kinase
    8. NM_182471.3NP_872271.1  pyruvate kinase PKM isoform b

      See identical proteins and their annotated locations for NP_872271.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (b, also called M1) is shorter at the N-terminus compared to isoform c.
      Source sequence(s)
      AC020779, BC023592, BC035198, DC326232, DC376054
      Consensus CDS
      CCDS32285.1
      UniProtKB/Swiss-Prot
      P14618
      UniProtKB/TrEMBL
      A0A024R5Z9
      Conserved Domains (2) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:43527
      pyruv_kin; pyruvate kinase

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 108 details...Open this link in a new tab

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p7 Primary Assembly

    Genomic

    1. NC_000015.10 Reference GRCh38.p7 Primary Assembly

      Range
      72199029..72231624 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_005254445.4XP_005254502.1  pyruvate kinase PKM isoform X4

      See identical proteins and their annotated locations for XP_005254502.1

      UniProtKB/Swiss-Prot
      P14618
      UniProtKB/TrEMBL
      V9HWB8
      Conserved Domains (2) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:43527
      pyruv_kin; pyruvate kinase
    2. XM_017022313.1XP_016877802.1  pyruvate kinase PKM isoform X5

      UniProtKB/Swiss-Prot
      P14618
      UniProtKB/TrEMBL
      A0A024R5Z9
      Related
      ENSP00000456970.1, OTTHUMP00000249145, ENST00000568459.5, OTTHUMT00000420062
      Conserved Domains (2) summary
      cd00288
      Location:42530
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:43527
      pyruv_kin; pyruvate kinase
    3. XM_011521670.2XP_011519972.1  pyruvate kinase PKM isoform X3

      See identical proteins and their annotated locations for XP_011519972.1

      Conserved Domains (2) summary
      cd00288
      Location:77565
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:78562
      pyruv_kin; pyruvate kinase
    4. XM_006720570.1XP_006720633.1  pyruvate kinase PKM isoform X1

      Conserved Domains (2) summary
      cd00288
      Location:116604
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:117601
      pyruv_kin; pyruvate kinase
    5. XM_005254443.1XP_005254500.1  pyruvate kinase PKM isoform X2

      Conserved Domains (2) summary
      cd00288
      Location:102590
      Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...
      TIGR01064
      Location:103587
      pyruv_kin; pyruvate kinase

    RNA

    1. XR_001751314.1 RNA Sequence

    Alternate CHM1_1.1

    Genomic

    1. NC_018926.2 Alternate CHM1_1.1

      Range
      72609505..72642108 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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