CDC25 Ras family guanine nucleotide exchange factor CDC25 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CDC25
Gene description: Ras family guanine nucleotide exchange factor CDC25
Primary source: SGD:S000004301
Locus tag: YLR310C
See related: AllianceGenome:SGD:S000004301
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CTN1
Summary: Enables guanyl-nucleotide exchange factor activity. Involved in Ras protein signal transduction and traversing start control point of mitotic cell cycle. Located in several cellular components, including cytosol; endoplasmic reticulum membrane; and nucleus. Human ortholog(s) of this gene implicated in Noonan syndrome; Noonan syndrome 4; Noonan syndrome 9; and gingival fibromatosis. Orthologous to several human genes including SOS1 (SOS Ras/Rac guanine nucleotide exchange factor 1) and SOS2 (SOS Ras/Rho guanine nucleotide exchange factor 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (752224..756993, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Cdc25/Ras/cAMP-dependent protein kinase A signaling pathway regulates proline utilization in wine yeast Saccharomyces cerevisiae under a wine fermentation model.
Title: The Cdc25/Ras/cAMP-dependent protein kinase A signaling pathway regulates proline utilization in wine yeast Saccharomyces cerevisiae under a wine fermentation model.
Carried out stochastic simulations, in particular, the effect of the negative feedback loops on the activity of both Ira2 (a Ras-GAP) and Cdc25 (a Ras-GEF) proteins.
Title: Simulation of the Ras/cAMP/PKA pathway in budding yeast highlights the establishment of stable oscillatory states.
Data show that the Ras2p localization is regulated by PKA, and that PKA down-regulates Ras2p activity and the protein association between Cdc25p and Ras2-GTP, which is due to a reduced Ras2-GEF Cdc25p activity.
Title: The membrane localization of Ras2p and the association between Cdc25p and Ras2-GTP are regulated by protein kinase A (PKA) in the yeast Saccharomyces cerevisiae.
Glucose-induced Ras2 activation was investigated in mutants lacking the N-terminal domain of Cdc25 or the entire CDC25; results suggest that the signal did not sustain the glucose-induced increase of Ras2-GTP normally observed in a wild-type strain.
Title: The large N-terminal domain of Cdc25 protein of the yeast Saccharomyces cerevisiae is required for glucose-induced Ras2 activation.
Taken together, these results suggest that Cdc25 is involved in the regulation of cell size in the presence of different carbon sources.
Title: The N-terminal region of the Saccharomyces cerevisiae RasGEF Cdc25 is required for nutrient-dependent cell-size regulation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in Ras protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in Ras protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info
involved_in traversing start control point of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Ras family guanine nucleotide exchange factor CDC25

NP_013413.1

Membrane bound guanine nucleotide exchange factor; also known as a GEF or GDP-release factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; thermosensitivity of the cdc25-5 mutant is functionally complemented by human RASGRF1 or by a fragment of human SOS1 comprising the CDC25-related catalytic domain

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

752224..756993 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182198.1 → NP_013413.1 TPA: Ras family guanine nucleotide exchange factor CDC25 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013413.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYV4, P04821

UniProtKB/TrEMBL

A7A1J5, N1NZL3

Conserved Domains (3) summary

cd11883
Location:62 → 122

SH3_Sdc25; Src Homology 3 domain of Sdc25/Cdc25 guanine nucleotide exchange factors

smart00147
Location:1301 → 1543

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

smart00229
Location:1117 → 1246

RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif