GCN1 Gcn1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: GCN1
Gene description: Gcn1p
Primary source: SGD:S000003163
Locus tag: YGL195W
See related: AllianceGenome:SGD:S000003163
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AAS103; NDR1
Summary: Enables protein kinase regulator activity. Involved in negative regulation of TORC1 signaling; positive regulation of macroautophagy; and regulation of translational elongation. Located in cytosolic ribosome. Orthologous to human GCN1 (GCN1 activator of EIF2AK4). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (131525..139543)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A genetic approach to identify amino acids in Gcn1 required for Gcn2 activation.
Title: A genetic approach to identify amino acids in Gcn1 required for Gcn2 activation.
Frameshifting at collided ribosomes is modulated by elongation factor eEF3 and by integrated stress response regulators Gcn1 and Gcn20.
Title: Frameshifting at collided ribosomes is modulated by elongation factor eEF3 and by integrated stress response regulators Gcn1 and Gcn20.
Structure of Gcn1 bound to stalled and colliding 80S ribosomes.
Title: Structure of Gcn1 bound to stalled and colliding 80S ribosomes.
Gcn1 contacts ribosome-bound Rps10 to efficiently mediate Gcn2 activation.
Title: Gcn1 contacts the small ribosomal protein Rps10, which is required for full activation of the protein kinase Gcn2.
Quantitative trait loci analysis suggest that polymorphism in the coding region of GCN1, MDS3, ARG81 and BIO3 could account for differences in nitrogen requirement.
Title: A genetic approach of wine yeast fermentation capacity in nitrogen-starvation reveals the key role of nitrogen signaling.
Yih1 binds with different requirements to distinct populations of Gcn1 molecules, and its ability to disrupt Gcn1-Gcn2 complexes is dependent on a complete RWD and hindered by actin binding.
Title: Gcn1 and actin binding to Yih1: implications for activation of the eIF2 kinase GCN2.
IMPACT bound to the minimal yeast GCN1 segment required for interaction with yeast GCN2 and YIH1 and to native mouse GCN1.
Title: IMPACT, a protein preferentially expressed in the mouse brain, binds GCN1 and inhibits GCN2 activation.
eEF3-related activity of GCN1 influences occupancy of the ribosomal decoding site by uncharged tRNA in starved cells.
Title: Polyribosome binding by GCN1 is required for full activation of eukaryotic translation initiation factor 2{alpha} kinase GCN2 during amino acid starvation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein kinase regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase regulator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables stalled ribosome sensor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in GCN2-mediated signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to amino acid starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of translation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in regulation of translational elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rescue of stalled ribosome	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosolic ribosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytosolic ribosome	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed

General protein information

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Preferred Names: Gcn1p

NP_011320.3

Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA; suppresses frameshifting at stalled ribosomes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

131525..139543

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181060.3 → NP_011320.3 TPA: Gcn1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011320.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTV8, P33892

UniProtKB/TrEMBL

A0A8H4FBH6, G2WDR1

Conserved Domains (4) summary

COG1413
Location:1567 → 1790

HEAT; HEAT repeat [General function prediction only]

sd00044
Location:1411 → 1435

HEAT; HEAT repeat [structural motif]

pfam12074
Location:392 → 670

Gcn1_N; Domain of unknown function (DUF3554)

pfam13646
Location:1575 → 1671

HEAT_2; HEAT repeats