THG1 tRNA guanylyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: THG1
Gene description: tRNA guanylyltransferase
Primary source: SGD:S000003256
Locus tag: YGR024C
See related: AllianceGenome:SGD:S000003256
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables tRNA guanylyltransferase activity. Involved in tRNA modification. Located in cytoplasm and nucleus. Used to study autosomal recessive cerebellar ataxia and cancer. Human ortholog(s) of this gene implicated in autosomal recessive cerebellar ataxia. Orthologous to human THG1L (tRNA-histidine guanylyltransferase 1 like). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (531883..532596, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Fidelity of base-pair recognition by a 3'-5' polymerase: mechanism of the Saccharomyces cerevisiae tRNA(His) guanylyltransferase.
Title: Fidelity of base-pair recognition by a 3'-5' polymerase: mechanism of the Saccharomyces cerevisiae tRNA(His) guanylyltransferase.
Minimal requirements for reverse polymerization and tRNA repair by tRNA(His) guanylyltransferase Thg1 have been presented.
Title: Minimal requirements for reverse polymerization and tRNA repair by tRNA(His) guanylyltransferase.
This study determined the crystal structure of GTP-bound Thg1 from Saccharomyces cerevisiae at the maximum resolution of 3.0 A.
Title: Crystal structure of tRNA(His) guanylyltransferase from Saccharomyces cerevisiae.
pyrophosphate removal versus nucleotide addition activities of yeast Thg1 with a set of tRNA(His) substrates
Title: Saccharomyces cerevisiae Thg1 uses 5'-pyrophosphate removal to control addition of nucleotides to tRNA(His.).
Study provides the first kinetic insight into the individual steps of the non-Watson-Crick addition of G- to tRNAHis, the known physiological function of Thg1 in yeast.
Title: Kinetic analysis of 3'-5' nucleotide addition catalyzed by eukaryotic tRNA(His) guanylyltransferase.
Thg1 substrate recognition is at least in part mediated by preventing recognition of incorrect substrates for nucleotide addition
Title: Identification of critical residues for G-1 addition and substrate recognition by tRNA(His) guanylyltransferase.
tRNAHis guanylyltransferase adds G-1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases
Title: tRNAHis guanylyltransferase adds G-1 to the 5' end of tRNAHis by recognition of the anticodon, one of several features unexpectedly shared with tRNA synthetases.
tRNA(His) from Thg1p-depleted cells is uncharged, although histidyl tRNA synthetase is active and the 3' end of the tRNA is intact, suggesting that G(-1) is a critical determinant for aminoacylation of tRNA(His) in vivo.
Title: Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C.
These results suggest that 3'-5' polymerization represents either an uncharacterized role for Thg1 in RNA or DNA repair or metabolism, or it may be a remnant of an earlier catalytic strategy used in nature.
Title: tRNAHis guanylyltransferase catalyzes a 3'-5' polymerization reaction that is distinct from G-1 addition.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-5' RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info
enables tRNA guanylyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables tRNA guanylyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables tRNA guanylyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in tRNA 5'-end processing	IEA Inferred from Electronic Annotation more info
involved_in tRNA modification	IDA Inferred from Direct Assay more info	PubMed
involved_in tRNA modification	IEA Inferred from Electronic Annotation more info
involved_in tRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in tRNA processing	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed

General protein information

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Preferred Names: tRNA guanylyltransferase

NP_011538.3

EC 2.7.7.79

tRNAHis guanylyltransferase; adds a guanosine residue to the 5' end of tRNAH is after transcription and RNase P cleavage; can also catalyze reverse (3'-5') polymerization with certain substrates in a template-dependent reaction; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

531883..532596 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181153.3 → NP_011538.3 TPA: tRNA guanylyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011538.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUF8, P53215, Q45U59

UniProtKB/TrEMBL

A0A6A5PV31, A6ZV13, B3LIJ5, B5VIY5, C7GK92, G2WEB5

Conserved Domains (1) summary

COG4021
Location:2 → 237

Thg1; tRNA(His) 5'-end guanylyltransferase [Translation, ribosomal structure and biogenesis]