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    TRZ1 tRNase Z [ Saccharomyces cerevisiae S288C ]

    Gene ID: 853954, updated on 11-Apr-2024

    Summary

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    Gene symbol
    TRZ1
    Gene description
    tRNase Z
    Primary source
    SGD:S000001787
    Locus tag
    YKR079C
    See related
    AllianceGenome:SGD:S000001787
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables 3'-tRNA processing endoribonuclease activity. Involved in mitochondrial tRNA 3'-end processing and tRNA 3'-trailer cleavage, endonucleolytic. Located in mitochondrion and nucleus. Used to study infantile hypertrophic cardiomyopathy and mitochondrial complex I deficiency. Human ortholog(s) of this gene implicated in combined oxidative phosphorylation deficiency 17. Orthologous to human ELAC2 (elaC ribonuclease Z 2). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: XI
    Exon count:
    1
    Sequence:
    Chromosome: XI; NC_001143.9 (586789..589305, complement)

    Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Msa2p Neighboring gene sorting nexin family protein Neighboring gene methylenetetrahydrofolate dehydrogenase (NAD(+)) Neighboring gene rRNA-binding ribosome biosynthesis protein RPF2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001143.9

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The crystal structure of Trz1, the long form RNase Z from yeast. Ma M, et al. Nucleic Acids Res, 2017 Jun 2. PMID 28379452, Free PMC Article
    2. Metal requirements and phosphodiesterase activity of tRNase Z enzymes. Späth B, et al. Biochemistry, 2007 Dec 25. PMID 18052196
    3. ELAC2 mutations cause a mitochondrial RNA processing defect associated with hypertrophic cardiomyopathy. Haack TB, et al. Am J Hum Genet, 2013 Aug 8. PMID 23849775, Free PMC Article
    4. Retrograde nuclear import of tRNA precursors is required for modified base biogenesis in yeast. Ohira T, et al. Proc Natl Acad Sci U S A, 2011 Jun 28. PMID 21670254, Free PMC Article
    5. Unstructured RNA is a substrate for tRNase Z. Shibata HS, et al. Biochemistry, 2006 May 2. PMID 16634630

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The 3.1 A crystal structure of the long-form Trz1 from Saccharomyces cerevisiae shows Trz1 is organized into 2 beta-lactamase domains connected by a long linker. The N-terminal domain has lost its catalytic residues, but keeps the long flexible arm important for tRNA binding, but it is the opposite in the C-terminal domain. Trz1 likely evolved from a duplication and fusion of the gene for the monomeric short form RNase Z.
      Title: The crystal structure of Trz1, the long form RNase Z from yeast.
    2. The yeast tRNA 3' endonuclease tRNase Z, Trz1, is catalyzing endonucleolytic tRNA 3' processing.
      Title: tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6.
    3. The findings reveal that 3' end processing is a limiting step for defective tRNA maturation and demonstrate that overexpression of scTRZ1 can promote defective tRNA 3' processing in vivo.
      Title: Functional conservation of tRNase ZL among Saccharomyces cerevisiae, Schizosaccharomyces pombe and humans.
    4. AthTRZ1 shows a high affinity for Zn2+ ions
      Title: Metal requirements and phosphodiesterase activity of tRNase Z enzymes.
    5. In this work, we show that the yeast homolog of ELAC2, encoded by TRZ1 (tRNase Z 1), is involved genetically in RNA processing.
      Title: Characterization of TRZ1, a yeast homolog of the human candidate prostate cancer susceptibility gene ELAC2 encoding tRNase Z.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables 3'-tRNA processing endoribonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3'-tRNA processing endoribonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3'-tRNA processing endoribonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in mitochondrial tRNA 3'-end processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitochondrial tRNA 3'-end processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in mitochondrial tRNA 3'-end processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA 3'-end processing IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tRNA 3'-end processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion HDA PubMed 
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus HDA PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    tRNase Z
    NP_013005.1
    • tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001143.9 Reference assembly

      Range
      586789..589305 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001179869.1 → NP_013005.1  TPA: tRNase Z [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013005.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VXD9, P36159
      UniProtKB/TrEMBL
      A0A8H4BY71, A7A038, C7GXD1
      Conserved Domains (3) summary
      cd07718
      Location:468 → 723
      RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold; Ribonuclease Z ELAC1, C-terminus of ELAC2, and related proteins; MBL-fold metallo-hydrolase domain
      pfam13691
      Location:6 → 68
      Lactamase_B_4; tRNase Z endonuclease
      cl00716
      Location:145 → 250
      tRNA_deacylase; D-aminoacyl-tRNA deacylase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P36159.1

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