RPN11 proteasome regulatory particle lid subunit RPN11 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: RPN11
Gene description: proteasome regulatory particle lid subunit RPN11
Primary source: SGD:S000001900
Locus tag: YFR004W
See related: AllianceGenome:SGD:S000001900
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MPR1
Summary: Enables metal-dependent deubiquitinase activity. Involved in several processes, including organelle fission; proteasome storage granule assembly; and proteolysis. Located in several cellular components, including cytosol; mitochondrion; and proteasome storage granule. Part of proteasome regulatory particle, lid subcomplex. Orthologous to human PSMD14 (proteasome 26S subunit, non-ATPase 14). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RPN11 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (153393..154313)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Report the ubiquitin-bound structure of Rpn11 from S. cerevisiae and the mechanisms for mechanochemical coupling of substrate degradation and deubiquitination.
Title: An AAA Motor-Driven Mechanical Switch in Rpn11 Controls Deubiquitination at the 26S Proteasome.
A key role for rpn11 in 26S proteasome
Title: Base-CP proteasome can serve as a platform for stepwise lid formation.
Fluorescently labeled tetra-Ub chains revealed endo-chain preference for Ubp6 acting on Lys(48) and random action for Rpn11.
Title: Disassembly of Lys11 and mixed linkage polyubiquitin conjugates provides insights into function of proteasomal deubiquitinases Rpn11 and Ubp6.
Overall, this work demonstrates that the co-opted Rpn11p, which is involved in the assembly of the functional proteasome, also functions in the proper assembly of the tombusvirus viral replicase complex.
Title: The proteasomal Rpn11 metalloprotease suppresses tombusvirus RNA recombination and promotes viral replication via facilitating assembly of the viral replicase complex.
Rpn11-Rpn8 interaction occurs via two distinct interfaces; Rpn11 lacks a conserved surface to bind the ubiquitin Ile44 patch
Title: Structure of the Rpn11-Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation.
The Rpn11 active site at the entrance to the ATPase ring pore is likely to prevent erroneous deubiquitylation of folded proteins.
Title: Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11.
Data show that the availability of Rpn11 is important for maintaining adequate levels of intact proteasomes, as its depletion caused growth and proteolytic defects in rpn3.
Title: A proteasome assembly defect in rpn3 mutants is associated with Rpn11 instability and increased sensitivity to stress.
The authors analyzed the rpn11-m1 phenotypes and found that overexpression of Cdc14 suppresses all the rpn11-m1 defects, including the mitochondrial ones.
Title: Analysis of the rpn11-m1 proteasomal mutant reveals connection between cell cycle and mitochondrial biogenesis.
It is conceivable that Rpn10/Rpn11 interaction couples proteasome assembly to substrate binding.
Title: Synthetic lethality of rpn11-1 rpn10Δ is linked to altered proteasome assembly and activity.
These results suggest that Mpr1-dependent arginine synthesis confers stress tolerance.
Title: An antioxidative mechanism mediated by the yeast N-acetyltransferase Mpr1: oxidative stress-induced arginine synthesis and its physiological role.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables cysteine-type deubiquitinase activity	IEA Inferred from Electronic Annotation more info
enables cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metal-dependent deubiquitinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metal-dependent deubiquitinase activity	IDA Inferred from Direct Assay more info	PubMed
enables metal-dependent deubiquitinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables metallopeptidase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables proteasome binding	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in mitochondrial fission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peroxisome fission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteasome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome storage granule assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein deubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein deubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein deubiquitination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein deubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome complex	IEA Inferred from Electronic Annotation more info
part_of proteasome regulatory particle, lid subcomplex	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome regulatory particle, lid subcomplex	IDA Inferred from Direct Assay more info	PubMed
located_in proteasome storage granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proteasome regulatory particle lid subunit RPN11

NP_116659.1

EC 3.4.19.12

Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

153393..154313

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179969.1 → NP_116659.1 TPA: proteasome regulatory particle lid subunit RPN11 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_116659.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTN4, P43588, Q8J0T5

UniProtKB/TrEMBL

A0A6A5PWR8, A7A242, B3LUL2, B5VI30, C7GYB0, C8Z7T7, G2WDE3, N1P4N6

Conserved Domains (1) summary

cd08069
Location:22 → 282

MPN_RPN11_CSN5; Mov34/MPN/PAD-1 family: proteasomal regulatory protein Rpn11 and signalosome complex subunit CSN5

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
3JCK_G.1
3JCO_V.1
3JCP_V.1
5MPB_V.1
5MPC_V.1
5MPD_V.1
5MPE_V.1
5WVI_V.1
5WVK_V.1
6EF3_r.1
6J2C_V.1
6J2N_V.1
6J2Q_V.1
6J2X_V.1
6J30_V.1
P43588.1