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    mdh malate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947854, updated on 23-Nov-2023

    Summary

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    Gene symbol
    mdh
    Gene description
    malate dehydrogenase
    Primary source
    ASAP:ABE-0010613
    Locus tag
    b3236
    See related
    ECOCYC:EG10576
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3225
    Summary
    Mdh is elevated 4X during growth at 4C (enabled by Oleispira antarctica Cpn10/60 transgene). [More information is available at EcoGene: EG10576]. Malate dehydrogenase (Mdh) catalyzes the reversible oxidation of malate to generate oxaloacetate. [More information is available at EcoCyc: EG10576].
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    Genomic context

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    Sequence:
    NC_000913.3 (3383330..3384268, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene periplasmic serine endoprotease Neighboring gene serine endoprotease Neighboring gene DNA-binding transcriptional dual regulator ArgR Neighboring gene DUF1471 domain-containing stress-induced protein YhcN

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Studying the Lysine Acetylation of Malate Dehydrogenase. Venkat S, et al. J Mol Biol, 2017 May 5. PMID 28366830, Free PMC Article
    2. Structure of Escherichia coli malate dehydrogenase at 1.45 A resolution. Zaitseva J, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2009 Sep 1. PMID 19724119, Free PMC Article
    3. Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase. Yin Y, et al. Proc Natl Acad Sci U S A, 2007 Oct 30. PMID 17947381, Free PMC Article
    4. Escherichia coli malate dehydrogenase, a novel solubility enhancer for heterologous proteins synthesized in Escherichia coli. Park JS, et al. Biotechnol Lett, 2007 Oct. PMID 17549433
    5. Direct transfer of NADH from malate dehydrogenase to complex I in Escherichia coli. Amarneh B, et al. Cell Biochem Biophys, 2005. PMID 15976458

    See all (50) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. GltA, icd and mdh do not form a transcription unit, are scattered around the chromosome and are expressed from different promoters.
      Title: Interaction of enzymes of the tricarboxylic acid cycle in Bacillus subtilis and Escherichia coli: a comparative study.
    2. The acetylation of lysine residues in MDH could enhance its enzyme activity.
      Title: Studying the Lysine Acetylation of Malate Dehydrogenase.
    3. The gene encoding malate dehydrogenase (MDH) was overexpressed in a pflB ldhA double mutant, NZN111, for succinic acid production.
      Title: Production of succinate by a pflB ldhA double mutant of Escherichia coli overexpressing malate dehydrogenase.
    4. N-terminus verified by Edman degradation on complete protein
      Title: '98 Escherichia coli SWISS-2DPAGE database update.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables L-malate dehydrogenase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables L-malate dehydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables malate dehydrogenase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables malate dehydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables malate dehydrogenase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables oxidoreductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within anaerobic respiration IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in carboxylic acid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within fermentation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within glycolytic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within malate metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in malate metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within tricarboxylic acid cycle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in tricarboxylic acid cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in extrinsic component of membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    malate dehydrogenase
    NP_417703.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3383330..3384268 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417703.1 malate dehydrogenase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417703.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7LA59, A0A8S7YAY1
      Conserved Domains (1) summary
      PRK05086
      Location:1 → 312
      PRK05086; malate dehydrogenase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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