Dapk2 death-associated protein kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dapk2provided by MGI
Official Full Name: death-associated protein kinase 2provided by MGI
Primary source: MGI:MGI:1341297
See related: Ensembl:ENSMUSG00000032380 AllianceGenome:MGI:1341297
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Predicted to enable several functions, including ATP binding activity; calmodulin binding activity; and identical protein binding activity. Involved in neutrophil migration. Predicted to be located in Golgi apparatus. Predicted to be active in cytoplasm and nucleus. Is expressed in clavicle; diencephalon lateral wall ventricular layer; mandible; maxilla; and orbito-sphenoid. Orthologous to human DAPK2 (death associated protein kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in lung adult (RPKM 10.8), placenta adult (RPKM 9.0) and 22 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 C; 9 35.75 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (66065364..66179524)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (66158226..66272242)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Thyroid hormone promotes selective autophagy via induction of DAPK2-SQSTM1 cascade, which in turn protects hepatocytes from diethylnitrosamine-induced hepatotoxicity or carcinogenesis.
Title: Thyroid hormone suppresses hepatocarcinogenesis via DAPK2 and SQSTM1-dependent selective autophagy.
This study links adipocyte expression of an autophagy-regulating kinase, lysosome-mediated clearance and fat cell lipid accumulation; it demonstrates obesity-related attenuated autophagy in adipocytes, and identifies DAPK2 dependence in this regulation.
Title: DAPK2 Downregulation Associates With Attenuated Adipocyte Autophagic Clearance in Human Obesity.
DAPK2 is strongly and specifically expressed in interstitial cells of the cortex, providing a useful marker for this important cell population
Title: Death associated protein kinase 2 is expressed in cortical interstitial cells of the mouse kidney.
The defect in chemotaxis in DAPK2-inactive granulocytes is likely a result of reduced polarization of the cells, mediated by a lack of MLC phosphorylation, resulting in radial F-actin and pseudopod formation.
Title: DAPK2 positively regulates motility of neutrophils and eosinophils in response to intermediary chemoattractants.
These results suggest that DAPK2 is one of the targets of cGK-I in apoptosis induction.
Title: cGMP-dependent protein kinase I promotes cell apoptosis through hyperactivation of death-associated protein kinase 2.
The crystal and solution structures of murine DAPK2 were determined in the presence of the autoinhibitory domain, with and without bound nucleotides in the active site. Dimers of DAPK2 had a conformation that did not permit protein substrate binding.
Title: Structure of the dimeric autoinhibited conformation of DAPK2, a pro-apoptotic protein kinase.
DAPK2 was substantially up-modulated during late erythropoiesis
Title: Attenuation of EPO-dependent erythroblast formation by death-associated protein kinase-2.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2)
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MHAa97c1.seq (e-PCR)
- UniSTS:125455

MT3700 (e-PCR)
- UniSTS:131797

D9Mit233.1 (e-PCR), detects polymorphism
- UniSTS:131798

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables calmodulin binding	ISO Inferred from Sequence Orthology more info
enables calmodulin binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in anoikis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in neutrophil migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of eosinophil chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neutrophil chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in regulation of intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of intrinsic apoptotic signaling pathway	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: death-associated protein kinase 2

Names: DAP kinase 2; DRP-1; death-associated kinase 2

NP_001391612.1

EC 2.7.11.1

NP_034149.2

EC 2.7.11.1

XP_036010490.1

EC 2.7.11.1

XP_036010491.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001404683.1 → NP_001391612.1 death-associated protein kinase 2 isoform 1

Status: VALIDATED

Source sequence(s)

AC124439

UniProtKB/TrEMBL

E9JGN0

Related

ENSMUSP00000158747.2, ENSMUST00000238682.2
NM_010019.4 → NP_034149.2 death-associated protein kinase 2 isoform 2

See identical proteins and their annotated locations for NP_034149.2

Status: VALIDATED

Source sequence(s)

AC124439

Consensus CDS

CCDS23303.1

UniProtKB/Swiss-Prot

O88861, Q8VDF3, Q9QYM4

Related

ENSMUSP00000034944.3, ENSMUST00000034944.9

Conserved Domains (1) summary

cd14196
Location:17 → 285

STKc_DAPK2; Catalytic domain of the Serine/Threonine Kinase, Death-Associated Protein Kinase 2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

66065364..66179524

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036154597.1 → XP_036010490.1 death-associated protein kinase 2 isoform X2

Conserved Domains (1) summary

cl21453
Location:1 → 130

PKc_like; Protein Kinases, catalytic domain
XM_036154598.1 → XP_036010491.1 death-associated protein kinase 2 isoform X2

Conserved Domains (1) summary

cl21453
Location:1 → 130

PKc_like; Protein Kinases, catalytic domain