Chrnb2 cholinergic receptor nicotinic beta 2 subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chrnb2provided by MGI
Official Full Name: cholinergic receptor nicotinic beta 2 subunitprovided by MGI
Primary source: MGI:MGI:87891
See related: Ensembl:ENSMUSG00000027950 AllianceGenome:MGI:87891
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Acrb2; Acrb-2; [b]2-nAchR; C030030P04Rik
Summary: Enables acetylcholine-gated cation-selective channel activity. Involved in regulation of synaptic vesicle exocytosis. Acts upstream of or within several processes, including learning or memory; nervous system development; and regulation of circadian sleep/wake cycle, sleep. Located in external side of plasma membrane. Is integral component of postsynaptic specialization membrane and integral component of presynaptic membrane. Part of acetylcholine-gated channel complex. Is active in cholinergic synapse and dopaminergic synapse. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Used to study attention deficit hyperactivity disorder; autism spectrum disorder; and autosomal dominant nocturnal frontal lobe epilepsy 3. Human ortholog(s) of this gene implicated in autosomal dominant nocturnal frontal lobe epilepsy and autosomal dominant nocturnal frontal lobe epilepsy 3. Orthologous to human CHRNB2 (cholinergic receptor nicotinic beta 2 subunit). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in whole brain E14.5 (RPKM 17.9), cerebellum adult (RPKM 14.9) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 F1; 3 39.19 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (89660755..89671939, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (89753448..89764632, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

alpha4beta2 nicotinic acetylcholine receptors intrinsically influence body weight in mice.
Title: α4β2 nicotinic acetylcholine receptors intrinsically influence body weight in mice.
Following alpha4beta2 nAChR stimulation, activated PKCbetaII translocated to the plasma membrane to induce clathrin-mediated endocytosis of D3R, resulting in downregulation and signal inhibition.
Title: α4β2 nicotinic acetylcholine receptor downregulates D(3) dopamine receptor expression through protein kinase C activation.
The majority of nicotinic receptors at the cell surface of superior cervical ganglia neurons consist of alpha3beta4alpha5 subunits.
Title: The role of the nAChR subunits α5, β2, and β4 on synaptic transmission in the mouse superior cervical ganglion.
Serine residues S336, S470 and S530 in Chrna4 protein have a brain region dependent modulatory role on surface alpha4beta2 nicotinic receptor expression.
Title: Serine residues in the α4 nicotinic acetylcholine receptor subunit regulate surface α4β2(*) receptor expression and clustering.
Acetylcholine tone fine-tunes the firing properties of Dopamine (DA) neurons of the ventral tegmental area through beta2-containing (beta2*) nicotinic Acetylcholine receptors.
Title: Manipulating midbrain dopamine neurons and reward-related behaviors with light-controllable nicotinic acetylcholine receptors.
Data suggest that receptor complex assembly from Chrna4 and Chrnb2 on neuronal cell surface is up-regulated by nicotine; combined effects of increased assembly stoichiometry and preferential trafficking likely drive up-regulation of this receptor complex on neuronal cell surface upon nicotine exposure. (Chrna4 = a4 nicotinic receptor; Chrnb2 = b2 nicotinic receptor)
Title: Organelle-specific single-molecule imaging of α4β2 nicotinic receptors reveals the effect of nicotine on receptor assembly and cell-surface trafficking.
study provides evidence that alpha6beta2 nAChRs are involved in locomotor activity as well as ethanol and saccharin consumption in adolescent animals
Title: α6β2 nicotinic acetylcholine receptors influence locomotor activity and ethanol consumption.
Results suggest that alpha4beta2( *) nAChRs can produce sustained regulation of inhibitory post-synaptic GABA currents, in fast-spiking layer V neurons of the mouse frontal area 2.
Title: α4β2(∗) nicotinic receptors stimulate GABA release onto fast-spiking cells in layer V of mouse prefrontal (Fr2) cortex.
beta2 containing nicotinic acetylcholine receptors play roles in the memory deficits associated with dentate-gyrys specific amyloid beta expression.
Title: A role for β2* nicotinic receptors in a model of local amyloid pathology induced in dentate gyrus.
our results show that the beta2V287L ADNFLE mutation increases the ACh sensitivities of both HS and LS alpha4beta2 nAChRs (but not that of alpha5alpha4beta2 nAChRs).
Title: Mutation Linked to Autosomal Dominant Nocturnal Frontal Lobe Epilepsy Reduces Low-Sensitivity α4β2, and Increases α5α4β2, Nicotinic Receptor Surface Expression.

Submit: New GeneRIF Correction See all GeneRIFs (85)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (7) 1 citation
Endonuclease-mediated (2)

General gene information

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Markers

Chrnb2 (e-PCR), detects polymorphism
- UniSTS:545914

AW822028 (e-PCR)
- UniSTS:207526

Chrnb2 (e-PCR), detects polymorphism
- UniSTS:256426

Chrnb2 (e-PCR), detects polymorphism
- UniSTS:141008

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acetylcholine binding	ISO Inferred from Sequence Orthology more info
enables acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IDA Inferred from Direct Assay more info	PubMed
enables acetylcholine-gated monoatomic cation-selective channel activity	IGI Inferred from Genetic Interaction more info	PubMed
enables acetylcholine-gated monoatomic cation-selective channel activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables acetylcholine-gated monoatomic cation-selective channel activity	ISO Inferred from Sequence Orthology more info
enables extracellular ligand-gated monoatomic ion channel activity	IEA Inferred from Electronic Annotation more info
contributes_to heterocyclic compound binding	ISO Inferred from Sequence Orthology more info
enables heterocyclic compound binding	ISO Inferred from Sequence Orthology more info
enables monoatomic ion channel activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables quaternary ammonium group binding	ISO Inferred from Sequence Orthology more info
enables transmembrane signaling receptor activity	IEA Inferred from Electronic Annotation more info
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IDA Inferred from Direct Assay more info	PubMed
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within B cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of B cell proliferation	IC Inferred by Curator more info	PubMed
acts_upstream_of B cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within acetylcholine receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in acetylcholine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of action potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within associative learning	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within behavioral response to nicotine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in behavioral response to nicotine	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within calcium ion transport	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within central nervous system neuron axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within central nervous system projection neuron axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in cognition	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within conditioned taste aversion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of dopamine secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of establishment of localization in cell	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lateral geniculate nucleus development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in learning	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane depolarization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within membrane depolarization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within memory	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in memory	ISO Inferred from Sequence Orthology more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within monoatomic ion transport	IEA Inferred from Electronic Annotation more info
involved_in monoatomic ion transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of neuronal action potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuronal action potential	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of neuronal action potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuronal action potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within optic nerve morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of B cell proliferation	IC Inferred by Curator more info	PubMed
acts_upstream_of_or_within positive regulation of B cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of dopamine secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of synaptic transmission, dopaminergic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in presynaptic modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of circadian sleep/wake cycle, REM sleep	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of circadian sleep/wake cycle, non-REM sleep	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of dopamine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of dopamine secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of membrane potential	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of synapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of synaptic transmission, dopaminergic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to acetylcholine	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to cocaine	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to ethanol	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to hypoxia	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to hypoxia	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to nicotine	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to nicotine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to nicotine	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sensory perception of pain	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within sensory perception of sound	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within smooth muscle contraction	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within social behavior	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within synaptic transmission involved in micturition	IGI Inferred from Genetic Interaction more info	PubMed
involved_in synaptic transmission, cholinergic	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within vestibulocochlear nerve development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within visual learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in visual learning	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within visual perception	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of acetylcholine-gated channel complex	IBA Inferred from Biological aspect of Ancestor more info
part_of acetylcholine-gated channel complex	IDA Inferred from Direct Assay more info	PubMed
part_of acetylcholine-gated channel complex	ISO Inferred from Sequence Orthology more info
is_active_in cholinergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in cholinergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in cholinergic synapse	ISO Inferred from Sequence Orthology more info
is_active_in dopaminergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in dopaminergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info	PubMed
located_in plasma membrane raft	ISO Inferred from Sequence Orthology more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic specialization membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic specialization membrane	ISO Inferred from Sequence Orthology more info
located_in presynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic membrane	IEP Inferred from Expression Pattern more info	PubMed
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: neuronal acetylcholine receptor subunit beta-2

Names: acetylcholine receptor beta 2 neural; cholinergic receptor, nicotinic, beta polypeptide 2 (neuronal)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009602.4 → NP_033732.2 neuronal acetylcholine receptor subunit beta-2 precursor

See identical proteins and their annotated locations for NP_033732.2

Status: VALIDATED

Source sequence(s)

AK051742, BC065103, BY123599

Consensus CDS

CCDS17515.1

UniProtKB/Swiss-Prot

Q8BGP7, Q9ERK7

UniProtKB/TrEMBL

Q9R291

Related

ENSMUSP00000029562.4, ENSMUST00000029562.5

Conserved Domains (3) summary

TIGR00860
Location:29 → 478

LIC; Cation transporter family protein

pfam02931
Location:29 → 234

Neur_chan_LBD; Neurotransmitter-gated ion-channel ligand binding domain

pfam02932
Location:241 → 477

Neur_chan_memb; Neurotransmitter-gated ion-channel transmembrane region

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

89660755..89671939 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036162861.1 → XP_036018754.1 neuronal acetylcholine receptor subunit beta-2 isoform X1

UniProtKB/TrEMBL

Q9R291

Conserved Domains (3) summary

TIGR00860
Location:2 → 418

LIC; Cation transporter family protein

cd03558
Location:95 → 109

LGIC_ECD; Cys-loop [structural motif]

cl28912
Location:1 → 174

LGIC_ECD; extracellular domain (ECD) of Cys-loop neurotransmitter-gated ion channels (also known as ligand-gated ion channel (LGIC))
XM_030252389.2 → XP_030108249.1 neuronal acetylcholine receptor subunit beta-2 isoform X1

UniProtKB/TrEMBL

Q9R291

Conserved Domains (3) summary

TIGR00860
Location:2 → 418

LIC; Cation transporter family protein

cd03558
Location:95 → 109

LGIC_ECD; Cys-loop [structural motif]

cl28912
Location:1 → 174

LGIC_ECD; extracellular domain (ECD) of Cys-loop neurotransmitter-gated ion channels (also known as ligand-gated ion channel (LGIC))