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    Asah2 N-acylsphingosine amidohydrolase 2 [ Mus musculus (house mouse) ]

    Gene ID: 54447, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Asah2provided by MGI
    Official Full Name
    N-acylsphingosine amidohydrolase 2provided by MGI
    Primary source
    MGI:MGI:1859310
    See related
    Ensembl:ENSMUSG00000024887 AllianceGenome:MGI:1859310
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Summary
    Enables N-acylsphingosine amidohydrolase activity. Involved in lipid digestion and sphingolipid metabolic process. Located in caveola; extracellular space; and mitochondrion. Is integral component of plasma membrane. Orthologous to several human genes including ASAH2B (N-acylsphingosine amidohydrolase 2B). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in small intestine adult (RPKM 19.0), large intestine adult (RPKM 15.0) and 18 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Asah2 in Genome Data Viewer
    Location:
    19 C1; 19 26.58 cM
    Exon count:
    24
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (31962046..32080540, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (31984646..32103140, complement)

    Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene protein kinase, cGMP-dependent, type I Neighboring gene RIKEN cDNA 8430431K14 gene Neighboring gene STARR-seq mESC enhancer starr_45921 Neighboring gene STARR-seq mESC enhancer starr_45922 Neighboring gene STARR-positive B cell enhancer ABC_E7645 Neighboring gene phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoribosylaminoimidazole, succinocarboxamide synthetase pseudogene Neighboring gene APOBEC1 complementation factor Neighboring gene VISTA enhancer mm1654 Neighboring gene STARR-positive B cell enhancer ABC_E5695 Neighboring gene STARR-positive B cell enhancer ABC_E4274 Neighboring gene STARR-seq mESC enhancer starr_45930 Neighboring gene STARR-seq mESC enhancer starr_45932 Neighboring gene coiled-coil domain containing 111 pseudogene Neighboring gene STARR-seq mESC enhancer starr_45936 Neighboring gene STARR-seq mESC enhancer starr_45939 Neighboring gene STARR-positive B cell enhancer ABC_E9456 Neighboring gene sphingomyelin synthase 1 Neighboring gene Sgms1 opposite strand transcript 1 Neighboring gene predicted gene, 38616

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Neutral ceramidase-dependent regulation of macrophage metabolism directs intestinal immune homeostasis and controls enteric infection. Sun R, et al. Cell Rep, 2022 Mar 29. PMID 35354041, Free PMC Article
    2. Neutral ceramidase deficiency protects against cisplatin-induced acute kidney injury. Sears SM, et al. J Lipid Res, 2022 Mar. PMID 35151662, Free PMC Article
    3. Role of neutral ceramidase in colon cancer. García-Barros M, et al. FASEB J, 2016 Dec. PMID 27609772, Free PMC Article
    4. Lactosylceramide contributes to mitochondrial dysfunction in diabetes. Novgorodov SA, et al. J Lipid Res, 2016 Apr. PMID 26900161, Free PMC Article
    5. Loss of neutral ceramidase protects cells from nutrient- and energy -deprivation-induced cell death. Sundaram K, et al. Biochem J, 2016 Mar 15. PMID 26747710, Free PMC Article

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Neutral ceramidase regulates breast cancer progression by metabolic programming of TREM2-associated macrophages.
      Title: Neutral ceramidase regulates breast cancer progression by metabolic programming of TREM2-associated macrophages.
    2. Neutral ceramidase-dependent regulation of macrophage metabolism directs intestinal immune homeostasis and controls enteric infection.
      Title: Neutral ceramidase-dependent regulation of macrophage metabolism directs intestinal immune homeostasis and controls enteric infection.
    3. Asah2 Represses the p53-Hmox1 Axis to Protect Myeloid-Derived Suppressor Cells from Ferroptosis.
      Title: Asah2 Represses the p53-Hmox1 Axis to Protect Myeloid-Derived Suppressor Cells from Ferroptosis.
    4. These results suggest that neutral ceramidase is a mediator of necroptosis and might be a novel therapeutic target for protection from ischaemic injury.
      Title: Loss of neutral ceramidase protects cells from nutrient- and energy -deprivation-induced cell death.
    5. Knocking down NCDase reduced sphingosine accumulation in mitochondria and preserved COX activity after the brain injury.
      Title: Essential roles of neutral ceramidase and sphingosine in mitochondrial dysfunction due to traumatic brain injury.
    6. Neutral ceramidase deficiency increases inflammation in a mouse model of inflammatory bowel disease.
      Title: Loss of neutral ceramidase increases inflammation in a mouse model of inflammatory bowel disease.
    7. Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.
      Title: Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.
    8. the predicted HNF-4alpha binding site identified in the Asah2 promoter is functional
      Title: Identification of a functional hepatocyte nuclear factor 4 binding site in the neutral ceramidase promoter.
    9. This enzyme is decreased in the small intestine in CFTR transgenic mice with a deletion in CFTR>
      Title: Expression of intestinal and lung alkaline sphingomyelinase and neutral ceramidase in cystic fibrosis f508del transgenic mice.
    10. Neutral ceramidase secreted by endothelial cells is released in part associated with caveolin-1.
      Title: Neutral ceramidase secreted by endothelial cells is released in part associated with caveolin-1.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (2)  1 citation
    • Endonuclease-mediated (2) 

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables N-acylsphingosine amidohydrolase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables N-acylsphingosine amidohydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables N-acylsphingosine amidohydrolase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables N-acylsphingosine amidohydrolase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables calcium ion binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ceramidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables dihydroceramidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables phytoceramidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within apoptotic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in ceramide biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ceramide biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in ceramide catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ceramide catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ceramide catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ceramide catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in lipid digestion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within lipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in long-chain fatty acid biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in response to organic substance ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within sphingolipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in sphingosine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in sphingosine biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in sphingosine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in sphingosine biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi apparatus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in caveola IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in extracellular exosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in extracellular region IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in extracellular space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in extracellular space ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    colocalizes_with membrane raft ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in mitochondrion HDA PubMed 
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in mitochondrion ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    neutral ceramidase
    Names
    N-CDase
    NCDase
    acylsphingosine deacylase 2
    neutral/alkaline ceramidase
    NP_061300.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_018830.1 → NP_061300.1  neutral ceramidase

      See identical proteins and their annotated locations for NP_061300.1

      Status: PROVISIONAL

      Source sequence(s)
      AB037181
      Consensus CDS
      CCDS29749.1
      UniProtKB/Swiss-Prot
      Q3UWP9, Q8BNP0, Q8BQN7, Q8R236, Q9JHE3
      UniProtKB/TrEMBL
      B7ZN55, B9EHG7
      Related
      ENSMUSP00000157744.2, ENSMUST00000236504.2
      Conserved Domains (3) summary
      PTZ00487
      Location:76 → 754
      PTZ00487; ceramidase; Provisional
      pfam04734
      Location:78 → 584
      Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal
      pfam17048
      Location:586 → 753
      Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000085.7 Reference GRCm39 C57BL/6J

      Range
      31962046..32080540 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9JHE3.1 GenPept UniProtKB/Swiss-Prot:Q9JHE3

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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