CSE4 centromeric DNA-binding histone H3-like protein CSE4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CSE4
Gene description: centromeric DNA-binding histone H3-like protein CSE4
Primary source: SGD:S000001532
Locus tag: YKL049C
See related: AllianceGenome:SGD:S000001532
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CSL2
Summary: Enables centromeric DNA binding activity. Involved in 2-micrometer plasmid partitioning; chromosome organization; and protein localization to CENP-A containing chromatin. Located in 2-micrometer circle DNA and kinetochore. Part of CENP-A containing nucleosome. Used to study cancer; male infertility; and oligospermia. Human ortholog(s) of this gene implicated in autoimmune disease. Orthologous to human CENPA (centromere protein A). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See CSE4 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (346075..346764, complement)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interaction of histone H4 with Cse4 facilitates conformational changes in Cse4 for its sumoylation and mislocalization.
Title: Interaction of histone H4 with Cse4 facilitates conformational changes in Cse4 for its sumoylation and mislocalization.
Recognition of centromere-specific histone Cse4 by the inner kinetochore Okp1-Ame1 complex.
Title: Recognition of centromere-specific histone Cse4 by the inner kinetochore Okp1-Ame1 complex.
Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability.
Title: Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability.
Cdc48Ufd1/Npl4 segregase removes mislocalized centromeric histone H3 variant CENP-A from non-centromeric chromatin.
Title: Cdc48Ufd1/Npl4 segregase removes mislocalized centromeric histone H3 variant CENP-A from non-centromeric chromatin.
Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.
Title: Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.
Reduced gene dosage of histone H4 prevents CENP-A mislocalization and chromosomal instability in Saccharomyces cerevisiae.
Title: Reduced gene dosage of histone H4 prevents CENP-A mislocalization and chromosomal instability in Saccharomyces cerevisiae.
Transcription destabilizes centromere function.
Title: Transcription destabilizes centromere function.
Cell-cycle phospho-regulation of the kinetochore.
Title: Cell-cycle phospho-regulation of the kinetochore.
Deposition of Centromeric Histone H3 Variant CENP-A/Cse4 into Chromatin Is Facilitated by Its C-Terminal Sumoylation.
Title: Deposition of Centromeric Histone H3 Variant CENP-A/Cse4 into Chromatin Is Facilitated by Its C-Terminal Sumoylation.
Study revealed that two essential Skp, Cullin, F-box (SCF) ubiquitin ligases defined by the F-box proteins Met30 and Cdc4 cooperatively regulate Cse4 (S. cerevisiae homolog of human centromere protein-A (CENP-A)) proteolysis under normal physiological conditions. Results suggest SCF-Met30/Cdc4-mediated proteolysis of Cse4 is one of the major mechanisms that prevents stable maintenance of Cse4 at non-centromeric regions.
Title: Skp, Cullin, F-box (SCF)-Met30 and SCF-Cdc4-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A for Chromosomal Stability in Budding Yeast.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables centromeric DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein heterodimerization activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of chromatin	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 2-micrometer plasmid partitioning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in kinetochore assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to CENP-A containing chromatin	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA transcription	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in 2-micrometer circle DNA	IDA Inferred from Direct Assay more info	PubMed
part_of CENP-A containing nucleosome	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in condensed chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in condensed chromosome, centromeric region	IGI Inferred from Genetic Interaction more info	PubMed
part_of nucleosome	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: centromeric DNA-binding histone H3-like protein CSE4

NP_012875.2

Centromeric histone H3-like protein; associates with promoters, accessible chromatin, and RNAPII-bound regions; Cse4p associates with centromeres, promoting kinetochore assembly and Sgo1p association with centromeric chromatin; Ipl1p-dependent phosphorylation destabilizes defective kinetochores promoting bi-orientation; phosphorylation by Cdc7p maintains Cse4p at kinetochores and promotes faithful chromosome segregation; regulated by multiple E3 ligases; complements human homolog CENPA mutations