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    relE mRNA interferase toxin RelE [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947549, updated on 27-Aug-2023

    Summary

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    Gene symbol
    relE
    Gene description
    mRNA interferase toxin RelE
    Primary source
    ASAP:ABE-0005221
    Locus tag
    b1563
    See related
    ECOCYC:EG11131
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1557
    Summary
    Overproduction of RelE has been shown to increase persistence. [More information is available at EcoGene: EG11131]. RelE is the toxin of the RelE-RelB toxin-antitoxin system . [More information is available at EcoCyc: EG11131].
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    Genomic context

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    Sequence:
    NC_000913.3 (1645346..1645633, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein Rem Neighboring gene toxic protein HokD Neighboring gene antitoxin/DNA-binding transcriptional repressor RelB Neighboring gene protein YdfV

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A ribosome profiling study of mRNA cleavage by the endonuclease RelE. Hwang JY, et al. Nucleic Acids Res, 2017 Jan 9. PMID 27924019, Free PMC Article
    2. Transition State Charge Stabilization and Acid-Base Catalysis of mRNA Cleavage by the Endoribonuclease RelE. Dunican BF, et al. Biochemistry, 2015 Dec 1. PMID 26535789, Free PMC Article
    3. Bacterial toxin RelE: a highly efficient ribonuclease with exquisite substrate specificity using atypical catalytic residues. Griffin MA, et al. Biochemistry, 2013 Dec 3. PMID 24251350, Free PMC Article
    4. Relaxed cleavage specificity within the RelE toxin family. Goeders N, et al. J Bacteriol, 2013 Jun. PMID 23543711, Free PMC Article
    5. Bacterial toxin RelE mediates frequent codon-independent mRNA cleavage from the 5' end of coding regions in vivo. Hurley JM, et al. J Biol Chem, 2011 Apr 29. PMID 21324908, Free PMC Article

    See all (57) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A ribosome profiling study of mRNA cleavage by the endonuclease RelE has been reported.
      Title: A ribosome profiling study of mRNA cleavage by the endonuclease RelE.
    2. The study uses a single-turnover kinetic analysis to measure the effect of pH and phosphorothioate substitution on the rate constant for cleavage of mRNA by wild-type RelE and seven active-site mutants.
      Title: Transition State Charge Stabilization and Acid-Base Catalysis of mRNA Cleavage by the Endoribonuclease RelE.
    3. RelE may principally promote catalysis via transition-state charge stabilization and leaving-group protonation, in addition to achieving in-line substrate positioning in cooperation with the ribosome.
      Title: Bacterial toxin RelE: a highly efficient ribonuclease with exquisite substrate specificity using atypical catalytic residues.
    4. RelE-like toxins have evolved to rapidly and efficiently shut down translation in a large spectrum of bacterial species, which correlates with the observation that toxin-antitoxin systems are spreading by horizontal gene transfer.
      Title: Relaxed cleavage specificity within the RelE toxin family.
    5. Here, the authors report that the transition to dormant states is mediated by one of the major toxin-antitoxin systems, RelEB, in a cell density-dependent manner in Escherichia coli K-12 MG1655.
      Title: RelE-mediated dormancy is enhanced at high cell density in Escherichia coli.
    6. RelE occupies the A site and causes cleavage of mRNA after the second nucleotide of the codon by reorienting and activating the mRNA for 2'-OH-induced hydrolysis.
      Title: The structural basis for mRNA recognition and cleavage by the ribosome-dependent endonuclease RelE.
    7. molecular interactions important for transcriptional control of the relBE model operon were characterized.
      Title: RelB and RelE of Escherichia coli form a tight complex that represses transcription via the ribbon-helix-helix motif in RelB.
    8. This study analyses mechanistic aspects of how RelE of Escherichia coli can function both as a co-repressor and as a derepressor of relBE transcription
      Title: Messenger RNA interferase RelE controls relBE transcription by conditional cooperativity.
    9. RelE specifically cleaves mRNA in the A site of the eukaryote ribosome.
      Title: The bacterial toxin RelE induces specific mRNA cleavage in the A site of the eukaryote ribosome.
    Submit: New GeneRIF Correction

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA-binding transcription repressor activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables DNA-binding transcription repressor activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ribosome binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables toxin sequestering activity EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables transcription cis-regulatory region binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within cellular response to amino acid starvation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within mRNA catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within negative regulation of translation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of growth NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within response to antibiotic IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in single-species biofilm formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of protein-DNA complex IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    part_of protein-DNA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of toxin-antitoxin complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    mRNA interferase toxin RelE

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1645346..1645633 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416081.1 mRNA interferase toxin RelE [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416081.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P0C077
      Conserved Domains (1) summary
      COG2026
      Location:1 → 87
      RelE; mRNA-degrading endonuclease RelE, toxin component of the RelBE toxin-antitoxin system [Defense mechanisms]
    Nucleotide Protein
    Heading Accession and Version

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