SPO11 DNA topoisomerase (ATP-hydrolyzing) [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: SPO11
Gene description: DNA topoisomerase (ATP-hydrolyzing)
Primary source: SGD:S000001014
Locus tag: YHL022C
See related: AllianceGenome:SGD:S000001014
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA end binding activity and chromatin binding activity. Involved in meiotic DNA double-strand break formation and synaptonemal complex assembly. Located in condensed nuclear chromosome. Orthologous to human SPO11 (SPO11 initiator of meiotic double stranded breaks). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (62961..64157, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Chromosome-dependent aneuploid formation in Spo11-less meiosis.
Title: Chromosome-dependent aneuploid formation in Spo11-less meiosis.
Concerted cutting by Spo11 illuminates meiotic DNA break mechanics.
Title: Concerted cutting by Spo11 illuminates meiotic DNA break mechanics.
Spo11 generates gaps through concerted cuts at sites of topological stress.
Title: Spo11 generates gaps through concerted cuts at sites of topological stress.
Structural and functional characterization of the Spo11 core complex.
Title: Structural and functional characterization of the Spo11 core complex.
Dual roles of yeast Rad51 N-terminal domain in repairing DNA double-strand breaks.
Title: Dual roles of yeast Rad51 N-terminal domain in repairing DNA double-strand breaks.
A tel1Delta mutant has globally increased amounts of Spo11-oligonucleotide complexes and altered Spo11-oligonucleotide lengths, consistent with conserved roles for Tel1 in control of DSB number and processing..We further find that effects of Tel1 are distinct but partially overlapping with previously described contributions of the recombination regulator Cst9 (also known as Zip3).
Title: Numerical and spatial patterning of yeast meiotic DNA breaks by Tel1.
Data show that increases in meiotic double-strand breaks and crossover frequencies can be programmed to arbitrary genomic sites by fusion of the Spo11 protein to various DNA-recognition domains.
Title: Programming sites of meiotic crossovers using Spo11 fusion proteins.
we found a role for the meiotic bouquet in establishing the size dependence of centromere coupling, as abolishing bouquet (using the bouquet-defective spo11 ndj1 mutant) reduces it. Coupling in spo11 ndj1 rather follows telomere clustering preferences. We propose that a chromosome size preference for centromere coupling helps establish efficient homolog recognition.
Title: Multiple Pairwise Analysis of Non-homologous Centromere Coupling Reveals Preferential Chromosome Size-Dependent Interactions and a Role for Bouquet Formation in Establishing the Interaction Pattern.
We examined fine-scale DSB distributions in TF mutant strains by deep sequencing oligonucleotides that remain covalently bound to Spo11 as a byproduct of DSB formation, mapped Bas1 and Ino4 binding sites in meiotic cells
Title: High-Resolution Global Analysis of the Influences of Bas1 and Ino4 Transcription Factors on Meiotic DNA Break Distributions in Saccharomyces cerevisiae.
These findings provide an explanation for the unexpectedly low prototroph levels exhibited by spo11 hypomorphs and have important implications for genetic studies that assume an unbiased recovery of prototrophs
Title: High throughput sequencing reveals alterations in the recombination signatures with diminishing Spo11 activity.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA end binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA topoisomerase activity	IEA Inferred from Electronic Annotation more info
enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in homologous chromosome pairing at meiosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic DNA double-strand break formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic DNA double-strand break formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic DNA double-strand break processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in reproductive process	IEA Inferred from Electronic Annotation more info
involved_in sexual reproduction	IEA Inferred from Electronic Annotation more info
involved_in sporulation resulting in formation of a cellular spore	IEA Inferred from Electronic Annotation more info
involved_in synaptonemal complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in nuclear chromosome	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA topoisomerase (ATP-hydrolyzing)

NP_011841.1

EC 5.6.2.2

Meiosis-specific protein required for meiotic recombination; makes DNA double-strand breaks (DSB) during meiotic recombination, homolog pairing, and synaptonemal complex formation; role in meiotic DSB formation involves a topo-II-like transesterase mechanism; forms monotetrameric complex; covalently linked to DSBs through a 5'-phosphodiester bond between a catalytic tyrosine (Y135) and the DSB 5'- end; sequence similarity to a topo VI subunit from archaea, fission yeast rec12 and human SPO11

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

62961..64157 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179102.1 → NP_011841.1 TPA: DNA topoisomerase (ATP-hydrolyzing) [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011841.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DKP3, P23179

UniProtKB/TrEMBL

A0A8H4F9I1, A6ZSM9, G2WF44

Conserved Domains (1) summary

COG1697
Location:31 → 398

Spo11; DNA topoisomerase VI, subunit A [Replication, recombination and repair]