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    PRKCA protein kinase C alpha [ Homo sapiens (human) ]

    Gene ID: 5578, updated on 29-Nov-2020

    Summary

    Official Symbol
    PRKCAprovided by HGNC
    Official Full Name
    protein kinase C alphaprovided by HGNC
    Primary source
    HGNC:HGNC:9393
    See related
    Ensembl:ENSG00000154229 MIM:176960
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha
    Summary
    Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in brain (RPKM 12.7), adrenal (RPKM 6.5) and 22 other tissues See more
    Orthologs

    Genomic context

    See PRKCA in Genome Data Viewer
    Location:
    17q24.2
    Exon count:
    24
    Annotation release Status Assembly Chr Location
    109.20201120 current GRCh38.p13 (GCF_000001405.39) 17 NC_000017.11 (66302613..66810743)
    105 previous assembly GRCh37.p13 (GCF_000001405.25) 17 NC_000017.10 (64298760..64806862)

    Chromosome 17 - NC_000017.11Genomic Context describing neighboring genes Neighboring gene RNA, 5S ribosomal pseudogene 444 Neighboring gene CRISPRi-validated cis-regulatory element chr17.4440 Neighboring gene Sharpr-MPRA regulatory region 10613 Neighboring gene RNA, 7SL, cytoplasmic 735, pseudogene Neighboring gene Sharpr-MPRA regulatory region 12714 Neighboring gene PRKCA antisense RNA 1 Neighboring gene RNA, U6 small nuclear 928, pseudogene Neighboring gene RNA, 5S ribosomal pseudogene 445 Neighboring gene microRNA 634 Neighboring gene calcium voltage-gated channel auxiliary subunit gamma 5 Neighboring gene RNA, 5S ribosomal pseudogene 446

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    Associated conditions

    Description Tests
    A genome-wide search for quantitative trait loci affecting the cortical surface area and thickness of Heschl's gyrus.
    GeneReviews: Not available
    Genetic association study of QT interval highlights role for calcium signaling pathways in myocardial repolarization.
    GeneReviews: Not available
    Genetics of coronary artery calcification among African Americans, a meta-analysis.
    GeneReviews: Not available
    Genome-wide association study identifies new susceptibility loci for posttraumatic stress disorder.
    GeneReviews: Not available
    Genome-wide association study of body height in African Americans: the Women's Health Initiative SNP Health Association Resource (SHARe).
    GeneReviews: Not available

    HIV-1 interactions

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env HIV-1 gp120-treated vaginal epithelial cells show downregulation of protein kinase C, alpha (PRKCA) expression as compared to untreated control PubMed
    env HIV-1 gp120 activates forward trafficking and surface clustering of NMDA receptors in membrane microdomains by a PKA-dependent phosphorylation of the NR1 C-terminal Ser897, followed by a PKC-dependent phosphorylation of Ser896 PubMed
    env Down modulation of the interaction between HIV-1 gp120 and CD4 by TPA is blocked by protein kinase C (PKC) inhibitors, suggesting PKC may play an important role in HIV-1 infection PubMed
    env PKC-dependent pathway, particularly PKCalpha and PKCbeta1, requires HIV-1 gp120-mediated Rac-1 activation and membrane fusion PubMed
    env c-FLIPL inhibits Bax activation via modulating PKC expression at the transcriptional level involving AP-2 during gp120 treatment PubMed
    env Pre-treatment of endothelial cells with fibroblast growth factor 2 (FGF2) protects cells from HIV-1 gp120 angiotoxicity; this protection is regulated by crosstalk among the ERK, PI3K-AKT and PKC signaling pathways PubMed
    env Induction of apoptosis in cell cultures through binding of HIV-1 gp120 or gp160 to CXCR4 involves protein kinase C, basic fibroblast growth factor, caspase-3, and the pro-apoptotic molecule Bax PubMed
    env HIV-1 gp120 increases the levels of calcium-dependent and -independent PKC isozymes; the most striking change is observed in PKC-zeta isozyme levels PubMed
    env A specific interaction between CD4 and HIV-1 gp120 is required for phosphorylation of CD4, which could involve protein kinase C PubMed
    env IL-16 induces rapid translocation of PKC from the cytosol to the membrane in CD4+ cells; PKC inhibitors completely block IL-16-induced lymphocyte migration as well as the motile response induced by HIV-1 gp120 and anti-CD4 antibody binding to CD4 PubMed
    Envelope surface glycoprotein gp160, precursor env HIV-1 gp160-induced Ca(2+) influx reduction is antagonized by an inhibitor acting especially on PKC alpha and PKC beta I; Western blotting analyses show that the cellular distribution of PKC alpha and -beta I are significantly modified by gp160 PubMed
    env HIV-1 gp160-induced AP-1 complex formation is dependent upon protein tyrosine phosphorylation and is abolished by inhibitors of protein kinase C, but it is unaffected by calcium channel blockers or cyclosporine A PubMed
    Envelope transmembrane glycoprotein gp41 env A synthetic peptide corresponding to cytoplasmic domain residues 828-848 of HIV-1 gp41 inhibits pKC-catalysed phosphorylation of a protein substrate PubMed
    env A synthetic peptide containing residues 581-597 from HIV-1 gp41 inhibits protein kinase C (pkC)-mediated phosphorylation of the CD3 gamma-chain in intact cells and directly inhibits partially purified pkC PubMed
    Nef nef PKC activity and serine 6 in HIV-1 Nef are required for Nef-supported transcription, infectivity, and replication PubMed
    nef Treatment of cells with protein kinase C (PKC) inhibitors reduces HIV-1 Nef phosphorylation in vivo, and phosphorylation of Nef increases 8- to 12-fold after treatment with phorbol myristate acetate; these data indicate PKC phosphorylates Nef PubMed
    nef Protein kinase C phosphorylates HIV-1 Nef at threonine 15 PubMed
    Rev rev PKC has been shown to phosphorylate Rev in vitro, an effect associated with accelerated formation of an efficient RNA-binding conformation for Rev PubMed
    Tat tat The gene expression of protein kinase C alpha subunit is significantly upregulated in both clade B and clade C Tat treated SK-N-MC neuroblastoma cells PubMed
    tat HIV-1 Tat downregulates the expression of protein kinase C, alpha (PRKCA) in human primary T cells PubMed
    tat Phospholipase C/protein kinase C signaling pathway-dependent phosphorylation of p44/42 and JNK MAP kinases participates partially in IL-1beta induction by TAT PubMed
    tat HIV-1 Tat-peptide(48-60) inhibits protein kinase C alpha and protein kinase A in a concentration-dependent manner PubMed
    tat Protein kinase C phosphorylates HIV-1 Tat on serine residue 46 PubMed
    tat Protein kinase C is required for HIV-1 Tat-mediated transactivation of the viral LTR promoter, indicating protein kinase C regulates the process of HIV-1 transactivation and may play a role in the transition of HIV from latency to productive growth PubMed
    tat HIV-1 Tat activates protein kinase C, resulting in the induction of TNF-alpha, IL-6 and IL-10 expression and the secretion of MCP-1 PubMed
    matrix gag Protein kinase C (PKC) phosphorylates HIV-1 Gag and Matrix on serine residue 111 resulting in a shift in localization of Matrix from the cytosol to the cellular membrane, suggesting a myristoyl-protein switch regulated by PKC phosphorylation PubMed
    retropepsin gag-pol Phosphorylation of human recombinant vimentin by PKC inhibits the proteolytic processing of the vimentin head domain by HIV-1 protease PubMed
    reverse transcriptase gag-pol HIV-1 RT heterodimer expressed in bacteria can be phosphorylated in vitro by several purified mammalian protein kinases including auto-activated protein kinase (PK), CKII, cytosolic protamine kinase (CPK), myelin basic protein kinase 1 (MBPK1), and PRKC PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    Clone Names

    • MGC129900, MGC129901

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    calcium-dependent protein kinase C activity IEA
    Inferred from Electronic Annotation
    more info
     
    enzyme binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    histone kinase activity (H3-T6 specific) IDA
    Inferred from Direct Assay
    more info
    PubMed 
    integrin binding ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    protein kinase C activity ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    protein kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    protein serine/threonine kinase activity EXP
    Inferred from Experiment
    more info
    PubMed 
    protein serine/threonine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    protein serine/threonine kinase activity TAS
    Traceable Author Statement
    more info
     
    zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    ERBB2 signaling pathway TAS
    Traceable Author Statement
    more info
     
    angiogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    apoptotic signaling pathway TAS
    Traceable Author Statement
    more info
    PubMed 
    axon guidance TAS
    Traceable Author Statement
    more info
     
    cell adhesion IEA
    Inferred from Electronic Annotation
    more info
     
    desmosome assembly IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    histone H3-T6 phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    intracellular signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    mitotic nuclear envelope disassembly TAS
    Traceable Author Statement
    more info
     
    negative regulation of glial cell apoptotic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    peptidyl-serine phosphorylation IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    peptidyl-serine phosphorylation TAS
    Traceable Author Statement
    more info
    PubMed 
    peptidyl-threonine phosphorylation ISS
    Inferred from Sequence or Structural Similarity
    more info
    PubMed 
    platelet activation TAS
    Traceable Author Statement
    more info
     
    positive regulation of ERK1 and ERK2 cascade ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    positive regulation of angiogenesis IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of blood vessel endothelial cell migration IDA
    Inferred from Direct Assay
    more info
    PubMed 
    positive regulation of bone resorption ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    positive regulation of cardiac muscle hypertrophy ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    positive regulation of cell adhesion IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of cell migration IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of dense core granule biogenesis ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    positive regulation of endothelial cell migration IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of endothelial cell proliferation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of lipopolysaccharide-mediated signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    positive regulation of macrophage differentiation ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    positive regulation of mitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    regulation of insulin secretion TAS
    Traceable Author Statement
    more info
     
    regulation of mRNA stability TAS
    Traceable Author Statement
    more info
     
    regulation of platelet aggregation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    response to interleukin-1 IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    Component Evidence Code Pubs
    alphav-beta3 integrin-PKCalpha complex ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytosol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytosol TAS
    Traceable Author Statement
    more info
     
    endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    PubMed 
    extracellular exosome HDA PubMed 
    mitochondrial membrane IEA
    Inferred from Electronic Annotation
    more info
     
    mitochondrion HDA PubMed 
    nucleoplasm TAS
    Traceable Author Statement
    more info
     
    perinuclear region of cytoplasm ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    plasma membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    plasma membrane TAS
    Traceable Author Statement
    more info
     

    General protein information

    Preferred Names
    protein kinase C alpha type
    Names
    PKC-A
    aging-associated gene 6
    NP_002728.2
    XP_016880325.1
    XP_016880326.1
    XP_016880330.1
    XP_024306597.1
    XP_024306598.1

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_012206.1 RefSeqGene

      Range
      5001..512937
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_002737.3NP_002728.2  protein kinase C alpha type

      Status: REVIEWED

      Source sequence(s)
      AC005918, AC005988, AC006263, AC006947, AC009452
      Consensus CDS
      CCDS11664.1
      Related
      ENSP00000408695.3, ENST00000413366.8
      Conserved Domains (3) summary
      cd04026
      Location:159289
      C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma
      cd05615
      Location:328668
      STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha
      pfam00130
      Location:3789
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

    RefSeqs of Annotated Genomes: Homo sapiens Updated Annotation Release 109.20201120

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p13 Primary Assembly

    Genomic

    1. NC_000017.11 Reference GRCh38.p13 Primary Assembly

      Range
      66302613..66810743
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017024836.2XP_016880325.1  protein kinase C alpha type isoform X1

    2. XM_017024841.1XP_016880330.1  protein kinase C alpha type isoform X4

    3. XM_017024837.1XP_016880326.1  protein kinase C alpha type isoform X2

    4. XM_024450830.1XP_024306598.1  protein kinase C alpha type isoform X3

      Conserved Domains (3) summary
      cd04026
      Location:73203
      C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma
      cd05615
      Location:242582
      STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha
      pfam00130
      Location:1668
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    5. XM_024450829.1XP_024306597.1  protein kinase C alpha type isoform X3

      Conserved Domains (3) summary
      cd04026
      Location:73203
      C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma
      cd05615
      Location:242582
      STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha
      pfam00130
      Location:1668
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

    RNA

    1. XR_001752558.1 RNA Sequence

      Related
      ENST00000578063.5
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