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PRKCH protein kinase C eta [ Homo sapiens (human) ]

Gene ID: 5583, updated on 15-Apr-2019

Summary

Official Symbol
PRKCHprovided by HGNC
Official Full Name
protein kinase C etaprovided by HGNC
Primary source
HGNC:HGNC:9403
See related
Ensembl:ENSG00000027075 MIM:605437
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PKCL; PKC-L; PRKCL; nPKC-eta
Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]
Expression
Ubiquitous expression in lymph node (RPKM 18.8), spleen (RPKM 14.7) and 24 other tissues See more
Orthologs

Genomic context

See PRKCH in Genome Data Viewer
Location:
14q23.1
Exon count:
23
Annotation release Status Assembly Chr Location
109 current GRCh38.p12 (GCF_000001405.38) 14 NC_000014.9 (61187305..61550980)
105 previous assembly GRCh37.p13 (GCF_000001405.25) 14 NC_000014.8 (61788515..62017698)

Chromosome 14 - NC_000014.9Genomic Context describing neighboring genes Neighboring gene RNA, U6 small nuclear 398, pseudogene Neighboring gene uncharacterized LOC101927756 Neighboring gene solute carrier family 38 member 6 Neighboring gene tRNA methyltransferase 5 Neighboring gene transmembrane protein 30B Neighboring gene uncharacterized LOC105370525 Neighboring gene long intergenic non-protein coding RNA 1303 Neighboring gene uncharacterized LOC400221 Neighboring gene HIF1A antisense RNA 1 Neighboring gene hypoxia inducible factor 1 subunit alpha

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Phenotypes

Associated conditions

Description Tests
Ischemic stroke
MedGen: C0948008 OMIM: 601367 GeneReviews: Not available
Compare labs

NHGRI GWAS Catalog

Description
A genome-wide association study for reading and language abilities in two population cohorts.
NHGRI GWA Catalog
Genetics of rheumatoid arthritis contributes to biology and drug discovery.
NHGRI GWA Catalog
Genome-wide SNP and CNV analysis identifies common and low-frequency variants associated with severe early-onset obesity.
NHGRI GWA Catalog
Meta-analysis identifies nine new loci associated with rheumatoid arthritis in the Japanese population.
NHGRI GWA Catalog

HIV-1 interactions

Replication interactions

Interaction Pubs
Knockdown of protein kinase C, eta (PRKCH) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 activates forward trafficking and surface clustering of NMDA receptors in membrane microdomains by a PKA-dependent phosphorylation of the NR1 C-terminal Ser897, followed by a PKC-dependent phosphorylation of Ser896 PubMed
env Down modulation of the interaction between HIV-1 gp120 and CD4 by TPA is blocked by protein kinase C (PKC) inhibitors, suggesting PKC may play an important role in HIV-1 infection PubMed
env c-FLIPL inhibits Bax activation via modulating PKC expression at the transcriptional level involving AP-2 during gp120 treatment PubMed
env Pre-treatment of endothelial cells with fibroblast growth factor 2 (FGF2) protects cells from HIV-1 gp120 angiotoxicity; this protection is regulated by crosstalk among the ERK, PI3K-AKT and PKC signaling pathways PubMed
env Induction of apoptosis in cell cultures through binding of HIV-1 gp120 or gp160 to CXCR4 involves protein kinase C, basic fibroblast growth factor, caspase-3, and the pro-apoptotic molecule Bax PubMed
env HIV-1 gp120 increases the levels of calcium-dependent and -independent PKC isozymes; the most striking change is observed in PKC-zeta isozyme levels PubMed
env A specific interaction between CD4 and HIV-1 gp120 is required for phosphorylation of CD4, which could involve protein kinase C PubMed
env IL-16 induces rapid translocation of PKC from the cytosol to the membrane in CD4+ cells; PKC inhibitors completely block IL-16-induced lymphocyte migration as well as the motile response induced by HIV-1 gp120 and anti-CD4 antibody binding to CD4 PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160-induced AP-1 complex formation is dependent upon protein tyrosine phosphorylation and is abolished by inhibitors of protein kinase C, but it is unaffected by calcium channel blockers or cyclosporine A PubMed
Envelope transmembrane glycoprotein gp41 env A synthetic peptide corresponding to cytoplasmic domain residues 828-848 of HIV-1 gp41 inhibits pKC-catalysed phosphorylation of a protein substrate PubMed
env A synthetic peptide containing residues 581-597 from HIV-1 gp41 inhibits protein kinase C (pkC)-mediated phosphorylation of the CD3 gamma-chain in intact cells and directly inhibits partially purified pkC PubMed
Nef nef The N-terminus of HIV-1 Nef associates with PKC eta PubMed
Tat tat Phospholipase C/protein kinase C signaling pathway-dependent phosphorylation of p44/42 and JNK MAP kinases participates partially in IL-1beta induction by TAT PubMed
tat Protein kinase C is required for HIV-1 Tat-mediated transactivation of the viral LTR promoter, indicating protein kinase C regulates the process of HIV-1 transactivation and may play a role in the transition of HIV from latency to productive growth PubMed
tat Protein kinase C phosphorylates HIV-1 Tat on serine residue 46 PubMed
tat HIV-1 Tat activates protein kinase C, resulting in the induction of TNF-alpha, IL-6 and IL-10 expression and the secretion of MCP-1 PubMed
matrix gag Protein kinase C (PKC) phosphorylates HIV-1 Matrix on serine residue 111 resulting in a shift in localization of Matrix from the cytosol to the cellular membrane, suggesting a myristoyl-protein switch regulated by PKC phosphorylation PubMed
retropepsin gag-pol Phosphorylation of human recombinant vimentin by PKC inhibits the proteolytic processing of the vimentin head domain by HIV-1 protease PubMed
reverse transcriptase gag-pol HIV-1 RT heterodimer expressed in bacteria can be phosphorylated in vitro by several purified mammalian protein kinases including auto-activated protein kinase (PK), CKII, cytosolic protamine kinase (CPK), myelin basic protein kinase 1 (MBPK1), and PRKC PubMed

Go to the HIV-1, Human Interaction Database

Pathways from BioSystems

  • Calcium Regulation in the Cardiac Cell, organism-specific biosystem (from WikiPathways)
    Calcium Regulation in the Cardiac Cell, organism-specific biosystemCalcium is a common signaling mechanism, as once it enters the cytoplasm it exerts allosteric regulatory affects on many enzymes and proteins. Calcium can act in signal transduction after influx resu...
  • Effects of PIP2 hydrolysis, organism-specific biosystem (from REACTOME)
    Effects of PIP2 hydrolysis, organism-specific biosystemHydrolysis of phosphatidyl inositol-bisphosphate (PIP2) by phospholipase C (PLC) produces diacylglycerol (DAG) and inositol triphosphate (IP3). Both are potent second messengers. IP3 diffuses into th...
  • Endothelins, organism-specific biosystem (from Pathway Interaction Database)
    Endothelins, organism-specific biosystem
    Endothelins
  • G Protein Signaling Pathways, organism-specific biosystem (from WikiPathways)
    G Protein Signaling Pathways, organism-specific biosystemG proteins, short for guanine nucleotide-binding proteins, are a family of proteins involved in second messenger cascades. G proteins are so called because they function as "molecular switches". They...
  • G alpha (q) signalling events, organism-specific biosystem (from REACTOME)
    G alpha (q) signalling events, organism-specific biosystemThe classic signalling route for G alpha (q) is activation of phospholipase C beta thereby triggering phosphoinositide hydrolysis, calcium mobilization and protein kinase C activation. This provides ...
  • G alpha (z) signalling events, organism-specific biosystem (from REACTOME)
    G alpha (z) signalling events, organism-specific biosystemThe heterotrimeric G protein Gz, is a member of the Gi family. Unlike other Gi family members it lacks an ADP ribosylation site cysteine four residues from the carboxyl terminus and is thus pertussis...
  • GPCR downstream signaling, organism-specific biosystem (from REACTOME)
    GPCR downstream signaling, organism-specific biosystemG protein-coupled receptors (GPCRs) are classically defined as the receptor, G-protein and downstream effectors, the alpha subunit of the G-protein being the primary signaling molecule. However, it h...
  • Gastrin-CREB signalling pathway via PKC and MAPK, organism-specific biosystem (from REACTOME)
    Gastrin-CREB signalling pathway via PKC and MAPK, organism-specific biosystemGastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic fac...
  • Hemostasis, organism-specific biosystem (from REACTOME)
    Hemostasis, organism-specific biosystemHemostasis is a physiological response that culminates in the arrest of bleeding from an injured vessel. Under normal conditions the vascular endothelium supports vasodilation, inhibits platelet adhe...
  • Inflammatory mediator regulation of TRP channels, organism-specific biosystem (from KEGG)
    Inflammatory mediator regulation of TRP channels, organism-specific biosystemThe TRP channels that exhibit a unique response to temperature have been given the name thermo-TRPs. Among all thermo- TRP channels, TRPV1-4, TRPM8, and TRPA1 are expressed in subsets of nociceptive...
  • Inflammatory mediator regulation of TRP channels, conserved biosystem (from KEGG)
    Inflammatory mediator regulation of TRP channels, conserved biosystemThe TRP channels that exhibit a unique response to temperature have been given the name thermo-TRPs. Among all thermo- TRP channels, TRPV1-4, TRPM8, and TRPA1 are expressed in subsets of nociceptive...
  • Insulin Signaling, organism-specific biosystem (from WikiPathways)
    Insulin Signaling, organism-specific biosystemInsulin signaling influences energy metabolism as well as growth. The presence of insulin signals the fed state, and this signal is passed via the AKT branch, which leads to the uptake of glucose fro...
  • MAPK Signaling Pathway, organism-specific biosystem (from WikiPathways)
    MAPK Signaling Pathway, organism-specific biosystemThe mitogen-activated protein kinase (MAPK) cascade is a highly conserved module that is involved in various cellular functions, including cell proliferation, differentiation and migration. Mammals e...
  • Myometrial Relaxation and Contraction Pathways, organism-specific biosystem (from WikiPathways)
    Myometrial Relaxation and Contraction Pathways, organism-specific biosystemThis pathway illustrates signaling networks implicated in uterine muscle contraction at labor and quiescence throughout gestation (pregnancy). The muscle of the uterus, responsible for contractile ac...
  • Oncostatin M Signaling Pathway, organism-specific biosystem (from WikiPathways)
    Oncostatin M Signaling Pathway, organism-specific biosystemOncostatin M (OSM) is a member of the multifunctional cytokine interleukin 6 (IL6) - type cytokine family. It is mainly produced in cell types such as activated T lymphocytes, macrophages, monocytes,...
  • Platelet activation, signaling and aggregation, organism-specific biosystem (from REACTOME)
    Platelet activation, signaling and aggregation, organism-specific biosystemPlatelet activation begins with the initial binding of adhesive ligands and of the excitatory platelet agonists (released or generated at the sites of vascular trauma) to cognate receptors on the pla...
  • Role of Calcineurin-dependent NFAT signaling in lymphocytes, organism-specific biosystem (from Pathway Interaction Database)
    Role of Calcineurin-dependent NFAT signaling in lymphocytes, organism-specific biosystem
    Role of Calcineurin-dependent NFAT signaling in lymphocytes
  • Signal Transduction, organism-specific biosystem (from REACTOME)
    Signal Transduction, organism-specific biosystemSignal transduction is a process in which extracellular signals elicit changes in cell state and activity. Transmembrane receptors sense changes in the cellular environment by binding ligands, such a...
  • Signaling Pathways in Glioblastoma, organism-specific biosystem (from WikiPathways)
    Signaling Pathways in Glioblastoma, organism-specific biosystemThe most frequently altered genes in glioblastoma. This pathway originally accompanied the 2008 Nature publication on the comprehensive genomic characterization of human glioblastoma genes and core p...
  • Signaling by GPCR, organism-specific biosystem (from REACTOME)
    Signaling by GPCR, organism-specific biosystemG protein-coupled receptors (GPCRs; 7TM receptors; seven transmembrane domain receptors; heptahelical receptors; G protein-linked receptors [GPLR]) are the largest family of transmembrane receptors i...
  • Thromboxane A2 receptor signaling, organism-specific biosystem (from Pathway Interaction Database)
    Thromboxane A2 receptor signaling, organism-specific biosystem
    Thromboxane A2 receptor signaling
  • Vascular smooth muscle contraction, organism-specific biosystem (from KEGG)
    Vascular smooth muscle contraction, organism-specific biosystemThe vascular smooth muscle cell (VSMC) is a highly specialized cell whose principal function is contraction. On contraction, VSMCs shorten, thereby decreasing the diameter of a blood vessel to regula...
  • Vascular smooth muscle contraction, conserved biosystem (from KEGG)
    Vascular smooth muscle contraction, conserved biosystemThe vascular smooth muscle cell (VSMC) is a highly specialized cell whose principal function is contraction. On contraction, VSMCs shorten, thereby decreasing the diameter of a blood vessel to regula...
  • Wnt Signaling Pathway, organism-specific biosystem (from WikiPathways)
    Wnt Signaling Pathway, organism-specific biosystemWnt proteins are secreted morphogens that are required for basic developmental processes, such as cell-fate specification, progenitor-cell proliferation and the control of asymmetric cell division, i...
  • Wnt Signaling Pathway and Pluripotency, organism-specific biosystem (from WikiPathways)
    Wnt Signaling Pathway and Pluripotency, organism-specific biosystemThis pathway was adapted from several resources and is designed to provide a theoretical frame-work for examining Wnt signaling and interacting components in the context of embryonic stem-cell plurip...
  • miRs in Muscle Cell Differentiation, organism-specific biosystem (from WikiPathways)
    miRs in Muscle Cell Differentiation, organism-specific biosystem
    miRs in Muscle Cell Differentiation

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Homology

Clone Names

  • MGC5363, MGC26269

Gene Ontology Provided by GOA

Function Evidence Code Pubs
ATP binding IEA
Inferred from Electronic Annotation
more info
 
enzyme binding IPI
Inferred from Physical Interaction
more info
PubMed 
metal ion binding IEA
Inferred from Electronic Annotation
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
protein kinase C activity IEA
Inferred from Electronic Annotation
more info
 
protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
Component Evidence Code Pubs
cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
cytosol ISS
Inferred from Sequence or Structural Similarity
more info
 
cytosol TAS
Traceable Author Statement
more info
 
extracellular exosome HDA PubMed 
plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
protein kinase C eta type
NP_006246.2
XP_011535256.1
XP_011535257.1
XP_011535258.1
XP_011535259.1
XP_016876947.1
XP_016876948.1
XP_024305429.1
XP_024305430.1

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_011514.1 RefSeqGene

    Range
    4647..234184
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_006255.4NP_006246.2  protein kinase C eta type

    See identical proteins and their annotated locations for NP_006246.2

    Status: REVIEWED

    Source sequence(s)
    AK290183, AL138996, AW008462, BC037268, BP367689
    Consensus CDS
    CCDS9752.1
    UniProtKB/Swiss-Prot
    P24723
    Related
    ENSP00000329127.5, ENST00000332981.10
    Conserved Domains (4) summary
    cd04014
    Location:8140
    C2_PKC_epsilon; C2 domain in Protein Kinase C (PKC) epsilon
    smart00220
    Location:355614
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd05590
    Location:359681
    STKc_nPKC_eta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C eta
    pfam00130
    Location:246298
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 109

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p12 Primary Assembly

Genomic

  1. NC_000014.9 Reference GRCh38.p12 Primary Assembly

    Range
    61187305..61550980
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_024449661.1XP_024305429.1  protein kinase C eta type isoform X4

    Conserved Domains (2) summary
    cd05590
    Location:198520
    STKc_nPKC_eta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C eta
    pfam00130
    Location:85137
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  2. XM_017021458.1XP_016876947.1  protein kinase C eta type isoform X4

    UniProtKB/Swiss-Prot
    P24723
    Related
    ENSP00000450981.1, ENST00000555082.5
    Conserved Domains (2) summary
    cd05590
    Location:198520
    STKc_nPKC_eta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C eta
    pfam00130
    Location:85137
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  3. XM_011536956.1XP_011535258.1  protein kinase C eta type isoform X3

    Conserved Domains (4) summary
    cd04014
    Location:8140
    C2_PKC_epsilon; C2 domain in Protein Kinase C (PKC) epsilon
    smart00220
    Location:355477
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    pfam00130
    Location:246298
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    cl21453
    Location:359492
    PKc_like; Protein Kinases, catalytic domain
  4. XM_011536957.1XP_011535259.1  protein kinase C eta type isoform X5

    Conserved Domains (4) summary
    cd04014
    Location:8140
    C2_PKC_epsilon; C2 domain in Protein Kinase C (PKC) epsilon
    smart00220
    Location:355478
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    pfam00130
    Location:246298
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    cl21453
    Location:359478
    PKc_like; Protein Kinases, catalytic domain
  5. XM_017021459.1XP_016876948.1  protein kinase C eta type isoform X6

  6. XM_024449662.1XP_024305430.1  protein kinase C eta type isoform X4

    Conserved Domains (2) summary
    cd05590
    Location:198520
    STKc_nPKC_eta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C eta
    pfam00130
    Location:85137
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  7. XM_011536954.3XP_011535256.1  protein kinase C eta type isoform X1

    Conserved Domains (3) summary
    smart00220
    Location:276535
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd05590
    Location:280602
    STKc_nPKC_eta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C eta
    pfam00130
    Location:167219
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
  8. XM_011536955.1XP_011535257.1  protein kinase C eta type isoform X2

    Conserved Domains (3) summary
    smart00220
    Location:275534
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd05590
    Location:279601
    STKc_nPKC_eta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C eta
    pfam00130
    Location:166218
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
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