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GTF2F2 general transcription factor IIF subunit 2 [ Homo sapiens (human) ]

Gene ID: 2963, updated on 25-Nov-2025
Official Symbol
GTF2F2provided by HGNC
Official Full Name
general transcription factor IIF subunit 2provided by HGNC
Primary source
HGNC:HGNC:4653
See related
Ensembl:ENSG00000188342 MIM:189969; AllianceGenome:HGNC:4653
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
BTF4; RAP30; TF2F2; TFIIF
Summary
Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in positive regulation of transcription by RNA polymerase II; transcription elongation by RNA polymerase II; and transcription initiation at RNA polymerase II promoter. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription factor TFIIF complex. [provided by Alliance of Genome Resources, Jul 2025]
Expression
Ubiquitous expression in skin (RPKM 5.5), adrenal (RPKM 5.1) and 25 other tissues See more
Orthologs
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See GTF2F2 in Genome Data Viewer
Location:
13q14.12-q14.13
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2025_08 current GRCh38.p14 (GCF_000001405.40) 13 NC_000013.11 (45120510..45284893)
RS_2025_08 current T2T-CHM13v2.0 (GCF_009914755.1) 13 NC_060937.1 (44340562..44504949)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 13 NC_000013.10 (45694645..45859028)

Chromosome 13 - NC_000013.11Genomic Context describing neighboring genes Neighboring gene RN7SK pseudogene 3 Neighboring gene uncharacterized LOC101929259 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5310 Neighboring gene H3K27ac hESC enhancer GRCh37_chr13:45694275-45694918 Neighboring gene H3K27ac hESC enhancer GRCh37_chr13:45694919-45695560 Neighboring gene ReSE screen-validated silencer GRCh37_chr13:45697873-45698066 Neighboring gene RN7SK pseudogene 4 Neighboring gene RNA, U6 small nuclear 69, pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5311 Neighboring gene uncharacterized LOC124903168 Neighboring gene potassium channel tetramerization domain containing 4 Neighboring gene uncharacterized LOC105370190 Neighboring gene OCT4-NANOG-H3K4me1 hESC enhancer GRCh37_chr13:45883380-45884072 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5312 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr13:45904287-45904787 Neighboring gene small nucleolar RNA, H/ACA box 31B Neighboring gene small nucleolar RNA, H/ACA box 31 Neighboring gene tumor protein, translationally-controlled 1

Go to nucleotide: Graphics FASTA GenBank

  • Project title: Tissue-specific circular RNA induction during human fetal development
  • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
  • BioProject: PRJNA270632
  • Publication: PMID 26076956
  • Analysis date: Mon Apr 2 22:54:59 2018

Related articles in PubMed

  1. Nucleotide and amino acid sequence of RAP30. Horikoshi M, et al. Nucleic Acids Res, 1991 Oct 11. PMID 1840667, Free PMC Article
  2. Structural homology between the Rap30 DNA-binding domain and linker histone H5: implications for preinitiation complex assembly. Groft CM, et al. Proc Natl Acad Sci U S A, 1998 Aug 4. PMID 9689043, Free PMC Article
  3. A region within the RAP74 subunit of human transcription factor IIF is critical for initiation but dispensable for complex assembly. Ren D, et al. Mol Cell Biol, 1999 Nov. PMID 10523626, Free PMC Article
  4. Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II. Sopta M, et al. Nature, 1989 Oct 5. PMID 2477704
  5. Distinct domains of hTAFII100 are required for functional interaction with transcription factor TFIIF beta (RAP30) and incorporation into the TFIID complex. Dubrovskaya V, et al. EMBO J, 1996 Jul 15. PMID 8758937, Free PMC Article

See all (145) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that TFIIF has an important role in stabilizing TFIIB within the PIC and after transcription initiates.
    Title: Transcription factor TFIIF is not required for initiation by RNA polymerase II, but it is essential to stabilize transcription factor TFIIB in early elongation complexes.
  2. NMR and thermodynamic studies further elucidate the complex molecular mechanism by which TFIIF and FCP1 cooperate for RNAPII recycling.
    Title: NMR structure of a complex formed by the carboxyl-terminal domain of human RAP74 and a phosphorylated peptide from the central domain of the FCP1 phosphatase.
  3. Results pinpoint critical residues for RNA polymerase II subunit 5 binding to RAP30 and/or to HBx, and identify these residues in both mammalian cells and in an in vitro binding assay.
    Title: Mutational analysis of human RNA polymerase II subunit 5 (RPB5): the residues critical for interactions with TFIIF subunit RAP30 and hepatitis B virus X protein.
Submit: New GeneRIF Correction

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env The transcription factors and kinases c-Jun, JNK, MEK, p38 MAPK, STAT-3, JAK-1, TFII D, TFII F, eIF-4E, and RNA polymerase II are induced by HIV-1 gp120 PubMed
Tat tat HIV-1 Tat interacts with the RNA polymerase II holoenzyme and transcription preinitiation complexes, which include TFIIF, during Tat-mediated transactivation of the HIV-1 LTR PubMed
tat TFIIF synergizes with HIV-1 Tat and the cellular co-activator Tat-SF1 during Tat-mediated transactivation of the HIV-1 LTR promoter PubMed
tat HIV-1 Tat directly interacts with the RAP30 subunit of TFIIF as demonstrated by Tat-affinity column purification PubMed
tat TFIIF is required for both basal and HIV-1 Tat-activated transcription PubMed

Go to the HIV-1, Human Interaction Database

Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA polymerase II general transcription initiation factor activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
  
involved_in transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in transcription by RNA polymerase II TAS
Traceable Author Statement
more info
 PubMed 
involved_in transcription elongation by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transcription elongation by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
  
involved_in transcription elongation by RNA polymerase II TAS
Traceable Author Statement
more info
 PubMed 
involved_in transcription initiation at RNA polymerase II promoter IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in transcription initiation at RNA polymerase II promoter IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transcription initiation at RNA polymerase II promoter IEA
Inferred from Electronic Annotation
more info
  
involved_in transcription initiation at RNA polymerase II promoter ISS
Inferred from Sequence or Structural Similarity
more info
  
Component Evidence Code Pubs
part_of RNA polymerase II transcription regulator complex IEA
Inferred from Electronic Annotation
more info
  
part_of RNA polymerase II, holoenzyme IEA
Inferred from Electronic Annotation
more info
  
located_in microtubule cytoskeleton IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus HDA PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of transcription factor TFIIF complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of transcription factor TFIIF complex IEA
Inferred from Electronic Annotation
more info
  
part_of transcription factor TFIIF complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
general transcription factor IIF subunit 2
Names
ATP-dependent helicase GTF2F2
TFIIF-beta
general transcription factor IIF 30 kDa subunit
general transcription factor IIF, polypeptide 2, 30kDa
transcription initiation factor IIF subunit beta
transcription initiation factor RAP30
NP_004119.1
XP_011533354.1
XP_011533355.1
XP_016876040.1
XP_054230456.1
XP_054230457.1
XP_054230458.1

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_004128.3 → NP_004119.1  general transcription factor IIF subunit 2

    See identical proteins and their annotated locations for NP_004119.1

    Status: VALIDATED

    Source sequence(s)
    AK291545, AL138963, BC001771, CA429230, CB124466, DB252856
    Consensus CDS
    CCDS9395.1
    UniProtKB/Swiss-Prot
    A6NNS5, P13984, Q5W0H3
    Related
    ENSP00000340823.6, ENST00000340473.8
    Conserved Domains (1) summary
    cl02122
    Location:6 → 247
    TFIIF_beta; Transcription initiation factor IIF, beta subunit

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2025_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000013.11 Reference GRCh38.p14 Primary Assembly

    Range
    45120510..45284893
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011535052.4 → XP_011533354.1  general transcription factor IIF subunit 2 isoform X1

    Related
    ENSP00000553090.1, ENST00000883031.1
    Conserved Domains (1) summary
    pfam02270
    Location:13 → 262
    TFIIF_beta; Transcription initiation factor IIF, beta subunit

  2. XM_011535053.4 → XP_011533355.1  general transcription factor IIF subunit 2 isoform X3

    Conserved Domains (1) summary
    cd07980
    Location:5 → 103
    TFIIF_beta; Transcription initiation factor IIF, beta subunit

  3. XM_017020551.2 → XP_016876040.1  general transcription factor IIF subunit 2 isoform X2

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060937.1 Alternate T2T-CHM13v2.0

    Range
    44340562..44504949
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054374481.1 → XP_054230456.1  general transcription factor IIF subunit 2 isoform X1

  2. XM_054374483.1 → XP_054230458.1  general transcription factor IIF subunit 2 isoform X3

  3. XM_054374482.1 → XP_054230457.1  general transcription factor IIF subunit 2 isoform X2

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P13984.2 GenPept UniProtKB/Swiss-Prot:P13984

Gene LinkOut

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