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EEF1A1 eukaryotic translation elongation factor 1 alpha 1 [ Homo sapiens (human) ]

Gene ID: 1915, updated on 10-Dec-2024

Summary

Official Symbol
EEF1A1provided by HGNC
Official Full Name
eukaryotic translation elongation factor 1 alpha 1provided by HGNC
Primary source
HGNC:HGNC:3189
See related
Ensembl:ENSG00000156508 MIM:130590; AllianceGenome:HGNC:3189
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; EF1A1; LENG7; eEF1A-1; GRAF-1EF; EF1alpha1
Summary
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in ovary (RPKM 2994.9), thyroid (RPKM 2551.7) and 25 other tissues See more
Orthologs
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Genomic context

See EEF1A1 in Genome Data Viewer
Location:
6q13
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 6 NC_000006.12 (73515750..73521032, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 6 NC_060930.1 (74692854..74698136, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 6 NC_000006.11 (74225473..74230755, complement)

Chromosome 6 - NC_000006.12Genomic Context describing neighboring genes Neighboring gene RNA, U6 small nuclear 975, pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24747 Neighboring gene mitochondrial tRNA translation optimization 1 Neighboring gene RNA, 7SL, cytoplasmic 827, pseudogene Neighboring gene MPRA-validated peak5897 silencer Neighboring gene Sharpr-MPRA regulatory region 3585 Neighboring gene H3K27ac hESC enhancer GRCh37_chr6:74229093-74229915 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74229916-74230737 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74230738-74231560 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17336 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17337 Neighboring gene EEF1A1 antisense RNA 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24751 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24752 Neighboring gene H3K27ac hESC enhancer GRCh37_chr6:74273247-74273796 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74275447-74275996 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74285738-74286453 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr6:74289636-74290835 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:74291165-74292007 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_94401 Neighboring gene solute carrier family 17 member 5 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_94402 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_94403 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_94405 Neighboring gene ribosomal protein S27 pseudogene 15

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIV-1 interactions

Replication interactions

Interaction Pubs
HIV-1 NL4-3 replication is restricted by EEF1A1 as enhanced replication was discovered when EEF1A1 is deleted through CRISPR/Cas9 genome editing in primary CD4+ T cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef eEF1A/Nef complexes contain tRNAs and block stress-induced apoptosis in monocyte-derived macrophages through tRNA binding to cytochrome c PubMed
nef Exp-t-mediated nuclear-cytoplasmic transport of the eEF1A/Nef complexes in monocyte-derived macrophages is invloved in the inhibition of apoptosis triggered by stress PubMed
nef HIV-1 Nef binds eEF1A in cells. Amino acids 55 to 206 in Nef and amino acids 1-74 in eEF1A are sufficient for the binding PubMed
nef Yeast two hybrid assay identifies the HIV-1 Nef-interacting protein translational elongation factor 1 PubMed
nef Tandem affinity purification and mass spectrometry analysis identify eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Cellular biotinylated eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) protein is incorporated into HIV-1 Gag virus-like particles PubMed
gag Tandem affinity purification and mass spectrometry analysis identify eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
gag The interaction between the Matrix protein of HIV-1 Gag and elongation factor 1-alpha impairs translation in vitro, suggesting a role for this interaction in releasing viral RNA from polysomes, permitting the RNA to be packaged into virions PubMed
gag The basic region of the Matrix protein of HIV-1 Gag (amino acids 15-35) mediates binding to amino acids 1-74 of elongation factor 1-alpha PubMed
gag Binding of elongation factor 1-alpha to HIV-1 Gag proteins (Matrix and Nucleocapsid) appears to be mediated by RNA PubMed
gag Elongation factor 1-alpha binds to HIV-1 Matrix, as well as to HIV-1 Gag and Nucleocapsid and is incorporated into HIV-1 virion membranes PubMed
Rev rev HIV-1 Rev interacting protein, eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells PubMed
Tat tat Eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) is identified to interact with HIV-1 Tat mutant Nullbasic in HeLa cells by LC MS/MS PubMed
tat EF-1alpha stimulates the binding of RNA Polymerase II and TRP-185 to HIV-1 TAR RNA, suggesting an interaction between EF-1alpha and HIV-1 Tat PubMed
integrase gag-pol HIV-1 IN co-immunoprecipitates with eEF1A through an interaction with the viral RTCs PubMed
gag-pol Using acetylated HIV-1 IN as bait in yeast two-hybrid screening identifies translation regulatory and RNA binding proteins eIF3h, eEF1A-1, and hnRNPA2 as IN-binding partners PubMed
gag-pol Interaction of human EF1alpha with HIV-1 integrase is presumed based on a reported binding interaction between yeast TEF1alpha and integrase PubMed
matrix gag The interaction between HIV-1 Matrix and elongation factor 1-alpha impairs translation in vitro, suggesting a role for this interaction in releasing viral RNA from polysomes, permitting the RNA to be packaged into virions PubMed
gag The basic region of HIV-1 Matrix (amino acids 15-35) mediates binding to amino acids 1-74 of elongation factor 1-alpha PubMed
gag Binding of elongation factor 1-alpha to HIV-1 Gag proteins (Matrix and Nucleocapsid) appears to be mediated by RNA PubMed
gag Elongation factor 1-alpha binds to HIV-1 Matrix, as well as to HIV-1 Gag and Nucleocapsid and is incorporated into HIV-1 virion membranes PubMed
reverse transcriptase p51 subunit gag-pol HIV-1 RT interacts with EEF1A1 as shown through biolayer interferometry and co-immunoprecipitation and can be abrogated by mutating amino acid W252 in RT PubMed
gag-pol HIV-1 RT p51 subunit co-immunoprecipitates with eEF1A through an interaction with the viral RTCs PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Clone Names

  • FLJ25721, MGC16224, MGC102687, MGC131894

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables GTP binding TAS
Traceable Author Statement
more info
PubMed 
enables GTPase activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables GTPase activity IDA
Inferred from Direct Assay
more info
PubMed 
enables RNA binding HDA PubMed 
enables calmodulin binding IEA
Inferred from Electronic Annotation
more info
 
enables kinase activator activity IDA
Inferred from Direct Assay
more info
PubMed 
enables kinase binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables molecular adaptor activity IDA
Inferred from Direct Assay
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein kinase binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables tRNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables translation elongation factor activity IBA
Inferred from Biological aspect of Ancestor
more info
 
enables translation elongation factor activity IDA
Inferred from Direct Assay
more info
PubMed 
enables translation elongation factor activity TAS
Traceable Author Statement
more info
PubMed 
Component Evidence Code Pubs
located_in cortical actin cytoskeleton IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytoplasm ISS
Inferred from Sequence or Structural Similarity
more info
 
located_in cytoplasm TAS
Traceable Author Statement
more info
PubMed 
located_in cytoplasmic side of lysosomal membrane NAS
Non-traceable Author Statement
more info
PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
 
is_active_in cytosolic ribosome IDA
Inferred from Direct Assay
more info
PubMed 
part_of eukaryotic translation elongation factor 1 complex TAS
Traceable Author Statement
more info
PubMed 
located_in extracellular exosome HDA PubMed 
located_in extracellular region TAS
Traceable Author Statement
more info
 
located_in extracellular space HDA PubMed 
located_in ficolin-1-rich granule lumen TAS
Traceable Author Statement
more info
 
located_in membrane HDA PubMed 
located_in nucleolus IDA
Inferred from Direct Assay
more info
PubMed 
located_in nucleus HDA PubMed 
NOT located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
PubMed 
is_active_in ribosome IDA
Inferred from Direct Assay
more info
PubMed 
located_in ruffle membrane IDA
Inferred from Direct Assay
more info
PubMed 
located_in secretory granule lumen TAS
Traceable Author Statement
more info
 

General protein information

Preferred Names
elongation factor 1-alpha 1
Names
CTCL tumor antigen
EF-1-alpha-1
EF1a-like protein
cervical cancer suppressor 3
elongation factor 1 alpha subunit
elongation factor Tu
epididymis secretory sperm binding protein
eukaryotic elongation factor 1 A-1
eukaryotic translation elongation factor 1 alpha 1-like 14
glucocorticoid receptor AF-1 specific elongation factor
leukocyte receptor cluster (LRC) member 7
leukocyte receptor cluster member 7
prostate tumor-inducing protein 1
translation elongation factor 1 alpha 1-like 14

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001402.6NP_001393.1  elongation factor 1-alpha 1

    See identical proteins and their annotated locations for NP_001393.1

    Status: REVIEWED

    Source sequence(s)
    AJ420488, AL603910, BC066893
    Consensus CDS
    CCDS4980.1
    UniProtKB/Swiss-Prot
    P04719, P04720, P68104, Q6IQ15
    UniProtKB/TrEMBL
    A8K9C4, Q53G85, Q53GA1, Q53GE9, Q53HM9, Q53HQ7, Q53HR5, Q6IPN6, Q6IPS9, Q6IPT9
    Related
    ENSP00000339053.4, ENST00000309268.11
    Conserved Domains (1) summary
    PTZ00141
    Location:1460
    PTZ00141; elongation factor 1- alpha; Provisional

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000006.12 Reference GRCh38.p14 Primary Assembly

    Range
    73515750..73521032 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060930.1 Alternate T2T-CHM13v2.0

    Range
    74692854..74698136 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054354427.1XP_054210402.1  elongation factor 1-alpha 1 isoform X1

    UniProtKB/Swiss-Prot
    P04719, P04720, P68104, Q6IQ15
    UniProtKB/TrEMBL
    A8K9C4, Q53G85, Q53GA1, Q53GE9, Q53HM9, Q53HQ7, Q53HR5, Q6IPN6, Q6IPS9, Q6IPT9

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_001403.1: Suppressed sequence

    Description
    NM_001403.1: This RefSeq was permanently suppressed because it was based on a chimeric sequence and represented a partial CDS.