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yhdJ DNA adenine methyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947695, updated on 10-Oct-2019

Summary

Gene symbol
yhdJ
Gene description
DNA adenine methyltransferase
Primary source
EcoGene:EG11498
Locus tag
b3262
See related
ASAP:ABE-0010706; ECOCYC:EG11498
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ccrM; ECK3249
Summary
In vitro DNA adenine methyltransferase activity has been demonstrated for YhdJ; overexpression of YhdJ leads to protection of chromosomal DNA from NsiI digestion, but DNA from wild type cells is not protected (Broadbent (2007). [More information is available at EcoGene: EG11498]. Overexpression of YdhJ leads to methylation of genomic DNA at the NsiI recognition sequence (5'-ATGCAT-3'), and partially purified YdhJ is shown to methylate this sequence to produce N6-methyladenine in the 3' adenine position of ATGCAT in vitro . ydhJ is not an essential gene, and overexpression of ydhJ does not alter cell morphology . [More information is available at EcoCyc: EG11498].

Genomic context

Sequence:
NC_000913.3 (3411653..3412537)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene tRNA-dihydrouridine synthase B Neighboring gene DNA-binding transcriptional dual regulator Fis Neighboring gene DUF2556 domain-containing protein YhdU Neighboring gene DNA-binding transcriptional repressor EnvR

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Gene Ontology Provided by GO

Function Evidence Code Pubs
DNA binding IEA
Inferred from Electronic Annotation
more info
 
N-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
 
methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
methyltransferase activity IEA
Inferred from Electronic Annotation
more info
 
nucleic acid binding IEA
Inferred from Electronic Annotation
more info
 
site-specific DNA-methyltransferase (adenine-specific) activity IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 
site-specific DNA-methyltransferase (adenine-specific) activity IDA
Inferred from Direct Assay
more info
PubMed 
site-specific DNA-methyltransferase (adenine-specific) activity IEA
Inferred from Electronic Annotation
more info
 
transferase activity IEA
Inferred from Electronic Annotation
more info
 
Process Evidence Code Pubs
DNA methylation IEA
Inferred from Electronic Annotation
more info
 
DNA methylation on adenine IEA
Inferred from Electronic Annotation
more info
 
methylation IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
PubMed 

General protein information

Preferred Names
DNA adenine methyltransferase
NP_417728.4

NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3411653..3412537
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417728.4 DNA adenine methyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417728.4

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P28638
    Conserved Domains (1) summary
    PRK11524
    Location:7290
    PRK11524; putative methyltransferase; Provisional
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