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    RNF185 ring finger protein 185 [ Homo sapiens (human) ]

    Gene ID: 91445, updated on 3-Nov-2024

    Summary

    Official Symbol
    RNF185provided by HGNC
    Official Full Name
    ring finger protein 185provided by HGNC
    Primary source
    HGNC:HGNC:26783
    See related
    Ensembl:ENSG00000138942 MIM:620096; AllianceGenome:HGNC:26783
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Summary
    Enables ubiquitin binding activity; ubiquitin protein ligase activity; and ubiquitin-like protein conjugating enzyme binding activity. Involved in several processes, including positive regulation of ERAD pathway; protein ubiquitination; and proteolysis involved in protein catabolic process. Located in endoplasmic reticulum. [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in thyroid (RPKM 37.5), prostate (RPKM 19.7) and 25 other tissues See more
    Orthologs
    NEW
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    Genomic context

    See RNF185 in Genome Data Viewer
    Location:
    22q12.2
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 22 NC_000022.11 (31160182..31207019)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 22 NC_060946.1 (31623670..31670571)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 22 NC_000022.10 (31556168..31603005)

    Chromosome 22 - NC_000022.11Genomic Context describing neighboring genes Neighboring gene Sharpr-MPRA regulatory region 9733 Neighboring gene SNRPN pseudogene 2 Neighboring gene microRNA 3928 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr22:31579617-31580130 Neighboring gene H3K27ac hESC enhancer GRCh37_chr22:31580131-31580643 Neighboring gene origin of replication in RNF185 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18849 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13624 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31608967-31609892 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18850 Neighboring gene LIM domain kinase 2 Neighboring gene RNA, U6 small nuclear 1128, pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18851 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31643479-31644369 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31644370-31645259 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31672194-31672774 Neighboring gene protein phosphatase 1 regulatory inhibitor subunit 14B pseudogene 1

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat HIV-1 Tat downregulates the expression of ring finger protein 185 (RNF185) in human primary T cells PubMed

    Go to the HIV-1, Human Interaction Database

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • FLJ38628

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein-containing complex binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables ubiquitin binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables ubiquitin protein ligase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables ubiquitin protein ligase activity TAS
    Traceable Author Statement
    more info
     
    enables ubiquitin-like protein conjugating enzyme binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables ubiquitin-like protein conjugating enzyme binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in endoplasmic reticulum membrane NAS
    Non-traceable Author Statement
    more info
    PubMed 
    located_in endoplasmic reticulum membrane TAS
    Traceable Author Statement
    more info
     
    located_in endoplasmic reticulum quality control compartment IEA
    Inferred from Electronic Annotation
    more info
     
    located_in mitochondrial outer membrane IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    E3 ubiquitin-protein ligase RNF185
    Names
    BSK65-MONO1
    BSK65-MONO2
    BSK65-PANC1
    BSK65-PANC2
    BSK65-TEST1
    BSK65-TEST2
    BSK65-TEST3
    RING-type E3 ubiquitin transferase RNF185
    NP_001129297.1
    NP_689480.2

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001135825.2NP_001129297.1  E3 ubiquitin-protein ligase RNF185 isoform 2

      See identical proteins and their annotated locations for NP_001129297.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) is lacking two in-frame coding exons compared to transcript variant 1, resulting in a shorter isoform (2) missing a 56 aa protein segment compared to isoform 1.
      Source sequence(s)
      AA258379, DB134817, DQ296562
      Consensus CDS
      CCDS46689.1
      Related
      ENSP00000266252.7, ENST00000266252.8
      Conserved Domains (1) summary
      cl17238
      Location:3764
      RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
    2. NM_152267.4NP_689480.2  E3 ubiquitin-protein ligase RNF185 isoform 1

      See identical proteins and their annotated locations for NP_689480.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1).
      Source sequence(s)
      AA258379, BC033166, DB134817
      Consensus CDS
      CCDS13890.1
      UniProtKB/Swiss-Prot
      A8K5C1, A9X3T8, Q8N900, Q96GF1
      Related
      ENSP00000320508.5, ENST00000326132.11
      Conserved Domains (1) summary
      cd16744
      Location:3880
      RING-HC_RNF185; RING finger, HC subclass, found in RING finger protein 185 (RNF185) and similar proteins

    RNA

    1. NR_024209.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) is missing an internal coding exon and contains an additional exon compared to transcript variant 1. This results in a frame-shift and premature translation termination, rendering the transcript susceptible to nonsense mediated mRNA decay (NMD). This transcript is sufficiently abundant to represent as a RefSeq record, however, the predicted protein is not represented because it is significantly truncated.
      Source sequence(s)
      AA258379, BC033166, BX648019, DB134817
    2. NR_024210.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (5) uses an alternate donor splice site at one of the coding exons compared to transcript variant 1. This results in a frame-shift and premature translation termination, rendering the transcript susceptible to nonsense mediated mRNA decay (NMD). This transcript is sufficiently abundant to represent as a RefSeq record, however, the predicted protein is not represented because it is truncated.
      Source sequence(s)
      AA258379, BC033166, DA498404, DB134817
    3. NR_024211.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (6) is missing an internal coding exon compared to transcript variant 1. This results in a frame-shift and premature translation termination, rendering the transcript susceptible to nonsense mediated mRNA decay (NMD). This transcript is sufficiently abundant to represent as a RefSeq record, however, the predicted protein is not represented because it is significantly truncated.
      Source sequence(s)
      AA258379, BC033166, BX648019, DB134817
    4. NR_024212.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (7) is missing a coding exon (containing the translation start site) compared to transcript variant 1. This results in the use of a downstream AUG in a different frame, and a predicted protein that is severely truncated with no similarity to this gene product. However, this transcript is sufficiently abundant to represent as a RefSeq record.
      Source sequence(s)
      AA258379, BC033166, DB134817
      Related
      ENST00000471384.5

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000022.11 Reference GRCh38.p14 Primary Assembly

      Range
      31160182..31207019
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060946.1 Alternate T2T-CHM13v2.0

      Range
      31623670..31670571
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001135824.1: Suppressed sequence

      Description
      NM_001135824.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript.